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Detection of air-borne inoculum of Caliciopsis pinea in a plantation of Pinus radiata in Tuscany, Italy
Bačová, Aneta
The genus Caliciopsis contains several representatives of the ascomycetous fungi which produce cankers on conifers. They comprise an interesting group with respect to morphology, physiology, and host response but have received relatively little attention in the past. The biology of this fungus is not totally elucidated. This study is focused on the description of seasonal spore dispersal of Caliciopsis pinea Peck and its relation to meteorological conditions which is needed for more precise and effective control of the disease. For this experiment the plantation of Pinus radiata in Carcheri (Lastra a Signa, Florence, Italy) was chosen. This plantation had symptoms of infection. A rotating arm spore trap and a weather station were installed here to obtain data from May to November 2016. Air samples were evaluated by qPCR with a very low detection limit. It has been found that spore traps are suitable for capturing the spore of this pathogen even with a very low incidence of inoculum in the air. Results show the presence of C. pinea inoculum throughout the entire sampling period with the highest peak in October. There is also a significant influence of precipitation on spore production. This work can help to better understand the life cycle of this pathogen. It can also contribute to enhance the control of this fungus and the protection of hosts.
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Hodnocení patogenity Hymenoscyphus fraxineus in vitro na různých živných médiích
Bačová, Aneta
This thesis deals with the pathogen Hymenoscyphus fraxineus causing the serious dieback of ash trees throughout Europe. In the first part, a summary of the state-of-art literature is presented. The main research is based on an in vitro examination of 15 strains of H. fraxineus, with the aim of finding the best medium for its growth. The five different nutrient media (MEA, AMEA, MOS, PDA, ADA) were chosen to cultivate the pathogen and examine its growth rate during six weeks. The medium ADA, composed of agar with of ash dust was designed and firstly used for this pathogen in our study. In addition, the relationship between the growth rate and the colour of mycelia was examined in three groups of strains (dark mycelium strains, light mycelium strains and strains with viruses). The fastest growth was found on the newly created ADA medium, which was obtained from ash twigs sawdust added to the classical agar medium. Dependence of the growth rate on mycelium colour has not been confirmed. This research contributes to the improvement of laboratory techniques designed to study H. fraxineus.
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Srovnání vlivu suburbanizace na regiony Tišnovska a Kuřimska a její vliv na plánování rozvoje území
Báčová, Aneta
BÁČOVÁ, A. Comparison of the impact of suburbanization on regions of Tišnov and Kuřim and its impact on spatial development planning. Master thesis. Brno: Mendel university. Faculty of Regional Development and International Studies, 2015. Thesis supervisor Mgr. Lukáš Nevěděl, Ph.D. The subject of this Master thesis is a comprehensive analysis of the administrative districts of municipalities with extended competence Tišnov and Kuřim affected by suburbanization processes and inner periphery. Using quantitative and cluster analysis this work evaluates demographic trends in the surveyed municipalities, their socioeconomic structure, the availability and capacity of public services. It also includes a prediction of the future population development.
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