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Biological effects of complex extracts from yeast and algae biomass
Bočán, David ; Byrtusová, Dana (referee) ; Skoumalová, Petra (advisor)
This bachelor thesis was focused on optimalization of extractions of active compounds from microalgae and carotenogenic yeasts. Other part of this work was focused on characterization of these extracts and their tests of cytotoxicity on human keratinocytes. In theoretical part a literary research which summarizes the basic properties and cultivation conditions of used microorganisms was conducted. Then there were listed an examples of antioxidant compounds found in these microorganisms as well as their properties. Finally the term cytotoxicity and methods of its measurement were clarified. The practical part of this thesis consists of series of extractions from mentioned microorganisms. These extracts were characterized by the content determination of phenolic and flavonoid compounds and also chlorophyll and carotenoid compounds. Determination of antioxidant capacity and SPF were done too. Lastly the cytotoxicity of chosen extracts was measured using the MTT test.
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Characterization of organic substances in biochar and their release in the soil ecosystem
Bočán, David ; Doležalová Weissmannová, Helena (referee) ; Mravcová, Ludmila (advisor)
The aim of this master’s thesis was to optimize the extraction of 38 PAH and 7 PCB compounds from samples of commercially available biochar and then apply the most suitable extraction method to real samples of biochar and soil. The concentrations of mentioned compounds were determined by using gas chromatography with mass spectrometry detection. Finally, from these concentrations in individual samples, a rate of release of these compounds from biochar to soil was determined. The theoretical part was focused on summarizing the process of biochar production and its properties. The next part deals with compounds that can be present in biochar, their physicochemical properties and effects on organisms and the environment. Also, a most common ways of extracting and determinating these compounds are mentioned. The final part focuses on soil and its characteristics and the changes it undergoes when biochar is applied to it. In the practical part an extensive optimization of extractions using a PSE extractor and thermal desorption extraction was conducted. After determining optimal conditions for both types of extraction methods, these methods were applied to real samples of biochar and soil. Qualitative analysis was carried out using GCxGC-TOF mass spectrometer and quantitative analysis by MRM method on GC-MS2 mass spectrometer.
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Characterization of organic substances in biochar and their release in the soil ecosystem
Bočán, David ; Doležalová Weissmannová, Helena (referee) ; Mravcová, Ludmila (advisor)
The aim of this master’s thesis was to optimize the extraction of 38 PAH and 7 PCB compounds from samples of commercially available biochar and then apply the most suitable extraction method to real samples of biochar and soil. The concentrations of mentioned compounds were determined by using gas chromatography with mass spectrometry detection. Finally, from these concentrations in individual samples, a rate of release of these compounds from biochar to soil was determined. The theoretical part was focused on summarizing the process of biochar production and its properties. The next part deals with compounds that can be present in biochar, their physicochemical properties and effects on organisms and the environment. Also, a most common ways of extracting and determinating these compounds are mentioned. The final part focuses on soil and its characteristics and the changes it undergoes when biochar is applied to it. In the practical part an extensive optimization of extractions using a PSE extractor and thermal desorption extraction was conducted. After determining optimal conditions for both types of extraction methods, these methods were applied to real samples of biochar and soil. Qualitative analysis was carried out using GCxGC-TOF mass spectrometer and quantitative analysis by MRM method on GC-MS2 mass spectrometer.
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Biological effects of complex extracts from yeast and algae biomass
Bočán, David ; Byrtusová, Dana (referee) ; Skoumalová, Petra (advisor)
This bachelor thesis was focused on optimalization of extractions of active compounds from microalgae and carotenogenic yeasts. Other part of this work was focused on characterization of these extracts and their tests of cytotoxicity on human keratinocytes. In theoretical part a literary research which summarizes the basic properties and cultivation conditions of used microorganisms was conducted. Then there were listed an examples of antioxidant compounds found in these microorganisms as well as their properties. Finally the term cytotoxicity and methods of its measurement were clarified. The practical part of this thesis consists of series of extractions from mentioned microorganisms. These extracts were characterized by the content determination of phenolic and flavonoid compounds and also chlorophyll and carotenoid compounds. Determination of antioxidant capacity and SPF were done too. Lastly the cytotoxicity of chosen extracts was measured using the MTT test.
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Knowledge of risks in health care by Faculty of Health and Social Sciences students
BOČAN, David
The diploma thesis deals with the identification of risks which threaten students in the fields of Radiology Assistant, General Nurse and Paramedic. The aim of the thesis is to map the knowledge of risks during providing health care in students of selected fields at the Faculty of Health and Social Studies at the University of South Bohemia. Four hypotheses were set in the thesis. Hypothesis no. 1 saying that there are statistically significant differences in risk knowledge between students of the first and last year of individual fields of health care. Hypothesis no. 2 that there are statistically significant differences in stress perception as a risk-enhancing factor between paramedics and general nurses. Hypothesis no. 3 states that there is a statistically significant difference in the knowledge of the risk of ionizing radiation between radiological assistants and general nurses and hypothesis no. 4 that paramedics will call their future occupation more risky than general nurses. To achieve the objectives and verify the hypotheses, a questionnaire was prepared, a questionnaire survey was conducted and everything was evaluated using graphic and statistical methods. The questionnaire contained 23 questions and the research sample consisted of 105 respondents from the studied fields. The results show that hypothesis no. 1 was confirmed in the fields of Radiology Assistant and Paramedic and rejected by the General Nurse. Hypothesis no. 2 was also confirmed. Hypothesis no. 3 was rejected and hypothesis no. 4 was also rejected. The contribution of the thesis is a complex picture of the knowledge of risks during providing health care in the studied fields. The results can serve the faculty to further more effective teaching.
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Dietary supplements in sport nutrition
BOČAN, David
Thesis deals with dietary supplements and their use by amateurs and professionals.The theoretical part is in the 1st part devoted to supplements and their legislation,forms and division.To the 2nd part was selected substances used by athletes and further discussed in terms of their function and effects on the organism.Within the general information was drafted in the 3rd part risks related to the use of dietary supplements due to wrong dosage or concentration and the issue of doping. Research was conducted through a questionnaire survey among professionals and amateurs.Questionnaire was created on the server google.cz and distributed in electronically and printed form.Respondents were south in several ways.There were used personal contacts and sending questionnaires to sport clubs.Research group contained 166 respondents-83 amateurs and 83 professionals.For evaluation were used basic statistical tests-pivot tables and chi-square test.The processing of results was made in Microsoft Excel 2013. Besides aims to map the use of supplements by athletes were expressed two hypotheses. The 1st hypothesis: There are statistically significant differences in knowledge of supplements between amateur and professional athletes.The 2nd hypothesis:There are statistically significant differences in the use of supplements between amateur and professional athletes. The 1st hypothesis was rejected.There were no statistically significant differences in knowledge of supplements between amateurs and professionals.The 2nd hypothesis was dealt separately for four seasonsfor the period of preparation before exercise/after exercise and for the period of the season, again before exercise/after exercise.In periods of preparation (before and after exercise) and after exercise in the season, there were statistically significant differences in the use of supplements between amateurs and professionals. In these cases 2nd hypothesis was confirmed.In the period of seasonbefore exercise was hypothesis rejected. In this period was no statistically significant differences in the use of supplements between amateurs and professionals. In the next question they were asked about learning about supplements.Most of the information were received from teammate/colleague. In the next part were surveyed effects of various factors for the purchase of supplements on a scale of 0 points (least)-10 points (most influence). Price is a factor that rather affects athletes but more the group of amateurs.Quality has a great influence on both groups. The country of origin is a factor which has by amateurs the largest representation at the point 0. Professionals find it like rather affecting. Brand name is a factor in both groups rather affecting. The package (label) doesn't rather affects athletes,more on amateurs. Recommendations known and previous experience are important for both groups of athletes.The influence of habit is not clear, almost all points are represented by the same ratio of votes. Discount and advertising are factors that affect athletes rather not.Influence expert forums and reviews on amateurs is not clear, professionals voted the most mean value. The most common reasons for using supplements are for both groups regeneration, energy source,adding vitamins and joint nutrition.Amateurs monthly invest in the purchase of dietary supplements less (500 CZK),professionals 500 or 1000 CZK. The most preferred forms of supplements are tablets,powders,capsules and liquids,including beverages.Sticks and gels are used more by professionals. Most of respondents use supplements by recommendation exactly or rather. The most common place of purchase supplements are for professionals specialized stores and for both groups also the Internet. Thesis could serve as an information resource for athletes to clarify that dietary supplements should take depending on what they want to achieve.Also it could be used as study material for courses dealing with nutrition and sport.
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