|
|
The effect of oxygen on the triplet state lifetime of carotenoids in photosynthetic complexes
Vahalová, Petra ; Pšenčík, Jakub (advisor) ; Bína, David (referee)
The aim of this study was to find a suitable method of preparation of anaerobic conditions in or- der to determine intrinsic lifetimes of carotenoid triplet states in photosynthetic light-harvesting complexes. We have compared the following three methods: (1) blowing an inert gas (nitrogen) above the surface of a liquid sample, (2) removing oxygen by means of chemical reactions catalyzed by glucose oxidase and catalase and (3) use of sodium dithionite. Each method was first tested on meso-tetra (4-sulfonatophenyl) porphine and then applied on the main light-harvesting complex of Amphidinium carterae. The lifetimes of the triplet states of carotenoids in the light-harvesting complex were determined from kinetics measured directly by a photomultiplier tube and from transient absorption spectra measured at different delays after the excitation pulse by an intensi- fied CCD camera. The effects of the used substances on the sample stability were evaluated by measuring steady-state absorption spectra and pH. Blowing the sample with nitrogen was time- consuming and less efficient in attaining anaerobic conditions compared to the other two methods. In contrast, anaerobic conditions were obtained in a relatively short time after the application of so- dium dithionite; however, it caused acidification of the sample ralated...
|
|
|
The effect of oxygen on the triplet state lifetime of carotenoids in photosynthetic complexes
Vahalová, Petra ; Pšenčík, Jakub (advisor) ; Bína, David (referee)
The aim of this study was to find a suitable method of preparation of anaerobic conditions in or- der to determine intrinsic lifetimes of carotenoid triplet states in photosynthetic light-harvesting complexes. We have compared the following three methods: (1) blowing an inert gas (nitrogen) above the surface of a liquid sample, (2) removing oxygen by means of chemical reactions catalyzed by glucose oxidase and catalase and (3) use of sodium dithionite. Each method was first tested on meso-tetra (4-sulfonatophenyl) porphine and then applied on the main light-harvesting complex of Amphidinium carterae. The lifetimes of the triplet states of carotenoids in the light-harvesting complex were determined from kinetics measured directly by a photomultiplier tube and from transient absorption spectra measured at different delays after the excitation pulse by an intensi- fied CCD camera. The effects of the used substances on the sample stability were evaluated by measuring steady-state absorption spectra and pH. Blowing the sample with nitrogen was time- consuming and less efficient in attaining anaerobic conditions compared to the other two methods. In contrast, anaerobic conditions were obtained in a relatively short time after the application of so- dium dithionite; however, it caused acidification of the sample ralated...
|
|
| |
|
| |
guest ::
RSS feed.