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Vliv kultivačních podmínek na kryoprezervaci půdních řas a sinic
Lukešová, Alena
Fundamental principles of both conservative and vitrification cryopreservation methods are presented, and factors affecting cryopreservation of algae and cyanobacteria are summarized. These factors include factors connected with the cryopreservation process itself as well as factors connected with pre- and post-cryopreservation manipulation with samples. Attention is paid to cultivation conditions, mainly to the temperature in the context of application of cold acclimation before cryopreservation. Both literature data and our experience indicate that the response of soil algae and cyanobacteria to particular factors is species or strain specific and optimization of condition can enhance post-cryopreservation viability of cryopreservation recalcitrant strain.

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