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Methods of detection of viruses from cereals and grasses using SYBR Green I RT-qPCR
Jarošová, Jana ; Dráb, Tomáš ; Beoni, Eva ; Kumar, Jiban
The family Poaceae is one of the most important groups of plants in the terms of agricultural production. The family Poaceae also hosts many pathogens including >100 known virus species. The distribution of the viruses varies according to hosts, geographical and other conditions. In this methodology we provide assays for detection of nine cereal and grass viruses (Brome mosaic virus-BMV, Barley yellow dwarf virus-BYDV, Lolium latent virus-LoLV, Oat necrotic mottle virus-ONMV, Ryegrass mosaic virus-Rg- MV, Soil-borne cereal mosaic virus-SBCMV, Spartina mottle virus-SpMV, Wheat dwarf virus-WDV a Wheat streak mosaic virus-WSMV) by SYBR Green I RT-qPCR.
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Methodics of molecular detection of Barley yellow dwarf virus in vectors by RT-PCR
Jarošová, Jana ; Jaňourová, Blanka ; Kumar, Jiban
Barley yellow dwarf is one of the most important viral diseases of cereals in the world. It can cause significant yield losses in most cereal species, including wheat, barley, rice, corn, oat and rye. In this methodology we provide assays for reverse transcription - polymerase chain reaction (RT-PCR) for sensitive detection of barley yellow dwarf virus directly in the vectors, in aphids. This way it is possible to evaluate infectivity of vectors and from that conclude subsequent measures. The methodology is meant to serve the employees of State Phytosanitary Administration.
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