National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Optofluidic techniques for directed evolution of enzymes
Pilát, Zdeněk ; Ježek, Jan ; Samek, Ota ; Zemánek, Pavel ; Buryška, T. ; Damborský, J. ; Prokop, Z.
Enzymes are highly versatile and ubiquitous biological catalysts. They can greatly accelerate\nlarge variety of reactions, while ensuring appropriate catalytic activity and high selectivity.\nThese properties make enzymes attractive biocatalysts for a wide range of industrial and\nbiomedical applications. Over the last two decades, directed evolution of enzymes has\ntransformed the field of protein engineering.
Fluorescence and surface enhanced Raman spectroscopy in microfluidics for monitoring of enzymatic reactions
Pilát, Zdeněk ; Šmatlo, Filip ; Ježek, Jan ; Krátký, Stanislav ; Zemánek, Pavel
We have implemented two different systems for detecting the concentration of molecules in microfluidic systems. The first method uses optical fibers and detects the intensity of fluorescence, while the second method is using surface enhanced Raman spectroscopy (SERS).
Experimental fluorescence device for dielectrophoretic sorting of droplets in microfluidic chips
Ježek, Jan ; Pilát, Zdeněk ; Šmatlo, Filip ; Zemánek, Pavel
At present, many chemical and biological disciplines use different forms of spectroscopy for their observations. One of the most common methods is fluorescence spectroscopy. During the last seven years, microfluidic techniques began developing rapidly, using channels in which two immiscible liquids create droplets with diameters from units to tens to hundreds of micrometers. These droplets serve as liquid microcontainers containing the analysed sample and the necessary reagents. Using special microfluidic techniques, it is possible to fuse droplets with different contents (controlled triggering of chemical reactions), to change the concentration of reactants in the droplet with high speed (concentration gradients), or sort the droplets by content (creation of new cell strains).\n\n

Interested in being notified about new results for this query?
Subscribe to the RSS feed.