National Repository of Grey Literature 7 records found  Search took 0.01 seconds. 
Detection and identification of aflatoxins and microcystins by means of portable liquid chromatograph for purpose of control of biological weapons prohibition
Lunerová, K. ; Šesták, Jozef
The methodology utilizes a portable liquid chromatograph for rapid detection and identification of selected aflatoxins and microcystins. It is primarily designated for SÚJCHBO, v. v. i. to control the compliance with the law 281/2002 Sb.
Detection and identification of chemical warfare agent simulants and degradation products by means of portable liquid chromatograph
Vaněk, J. ; Šesták, Jozef ; Lunerová, K. ; Tanzinger, L.
The methodology utilizes a portable liquid chromatograph for rapid detection and identification of selected chemical warfare agent simulants and degradation products. It is primarily designated for SÚJCHBO, v. v. i. to control compliance with the law 19/1997 Sb. and for research activity.
Development of divergent-flow isoelectric focusing chip for separation of biological agents
Duša, Filip ; Šalplachta, Jiří ; Horká, Marie ; Lunerová, K. ; Rosenbergová, K. ; Kubíček, O.
Isoelectric focusing (IEF) is an efficient method for separation of different bacterium species. Divergent flow format of IEF (DF-IEF) enables continual separation of large volumes of samples and fractionation of the separated analytes for later analysis. The mentioned properties enabled development of DF-IEF chip for separation of H2O2 inactivated biological agents (B-agents). The chip was designed to perform separation in vertical alignment which solved issues connected with sedimentation of bacterial cells. The separation method was optimized using colored markers of isoelectric point and four different b-agents were separated using the DF-IEF chip. The obtained fractions were subsequently analyzed with matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). The mass spectra proved that the developed DF-IEF chip is an effective tool for separation of inactivated B-agents.
Portable liquid chromatograph and analyses of hazardous substances
Šesták, Jozef ; Gogaľová, Zuzana ; Kahle, Vladislav ; Lunerová, K.
Progress in technology of microchips, batteries and LEDs in the last two decades enable development of portable instruments which can satisfy the requirements of modern liquid chromatography (LC). Application of portable LC is advantageous if simple and rapid sample treatment procedure followed by a short HPLC run can be executed in the point of sampling. Our research group have developed a concept of portable HPLC based on simple hydraulic scheme and a unique optical detector for simultaneous detection of absorbance and fluorescence of compounds eluted off microcolumn. Considering in-field analysis of hazardous substances, this instrument can be advantageous for analysis of compounds which are difficult to detect and quantify by other contemporary portable instruments utilizing different working principles due to incompatibility with their physico-chemical properties (e. g. microbial toxins).
Development of portable ultra-high performance liquid chromatograph
Šesták, Jozef ; Gogaľová, Zuzana ; Lunerová, K.
Recently we have successfully proved the concept of experimental portable liquid chromatograph which utilizes a simple hydraulic scheme and a universal optical detector capable of simultaneous absorbance and fluorescence monitoring. Here we present some latest improvements of our concept.
Separation of biological agents by divergent-flow isoelectric focusing as a tool for their rapid identification by MALDI-TOF MS
Duša, Filip ; Šalplachta, Jiří ; Horká, Marie ; Lunerová, K. ; Rosenbergová, K. ; Kubíček, O.
We presented DF-IEF chip which proved as an efficient method for separation of biological agents, and it enabled sensitive identification of inactivated microorganisms with MALDI-TOF MS compared to unseparated samples.
Portable liquid chromatograph Portable as a new tool for the determination of hazardous substances
Šesták, Jozef ; Lunerová, K. ; Vaněk, J.
The portable liquid chromatograph is a new instrument of compact dimensions, which enables the implementation of HPLC analyzes in conditions outside the laboratory. It was used for the development of a certified methodology for the detection and determination of selected mycotoxins in complex samples.\n\n

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