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National Repository of Grey Literature

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Diagnostic method for Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial wilt of tomato Kokošková, B. ; Mráz, Ivan ; Fousek, Jan The aim of this methodology was optimize detection and determination of quarantine bacterium Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato, The disease causes high ecomomic losses in main production areas on indeterminate greenhouse and fi eld tomatoes. Methodology is based on a combination of both optimized techniques utilizing different principles of action: immunochemical (IF) and molecular (PCR). The submitted diagnostic protocol alows to eliminate potential false negatives and positives and ensure the highest reliability of results. Specific and timely detection of Clavibacter michiganensis subsp. michiganensis will alow effective aplication of plant protection mesures. Detailed record

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