|
|
Interaction of the human pathogen Bordetella pertussis with blood serum
Štipl, Daniel ; Večerek, Branislav (advisor) ; Kamanová, Jana (referee)
Bordetella pertussis is a Gram-negative strictly human pathogen and the major causative agent of whooping cough or pertussis. The incidence of this highly contagious respiratory disease in developed countries has increased in the last decades. One of the less characterized virulence factors of B. pertussis is the type three secretion system (TTSS) which is responsible for the secretion of the effector proteins into host eukaryotic cells. This diploma thesis sheds light onto factors influencing TTSS in vitro activity. Although TTSS of laboratory strain Tohama I was induced by biologically active compounds present in blood (e. g. complement proteins), TTSS of recent clinical isolate B1917 seems to be induced permanently. Furthermore, BB0302 encoding a GntR family transcription regulator in B. bronchiseptica RB50 (homologous to BP0209 of Tohama I) was studied, however, the deletion of this gene did not affect the TTSS functionality. Serum resistance is a factor that plays a key role in the pathogenesis of B pertussis. We show that Czech recent isolates (2008-2015) are significantly more resistant to serum killing in vitro than the original vaccine strains (1954-1965). This phenomenon seems to result from the adaptation of global B. pertussis population to its human host. In addition, this diploma...
|
|
|
Interaction of the human pathogen Bordetella pertussis with blood serum
Štipl, Daniel ; Večerek, Branislav (advisor) ; Kamanová, Jana (referee)
Bordetella pertussis is a Gram-negative strictly human pathogen and the major causative agent of whooping cough or pertussis. The incidence of this highly contagious respiratory disease in developed countries has increased in the last decades. One of the less characterized virulence factors of B. pertussis is the type three secretion system (TTSS) which is responsible for the secretion of the effector proteins into host eukaryotic cells. This diploma thesis sheds light onto factors influencing TTSS in vitro activity. Although TTSS of laboratory strain Tohama I was induced by biologically active compounds present in blood (e. g. complement proteins), TTSS of recent clinical isolate B1917 seems to be induced permanently. Furthermore, BB0302 encoding a GntR family transcription regulator in B. bronchiseptica RB50 (homologous to BP0209 of Tohama I) was studied, however, the deletion of this gene did not affect the TTSS functionality. Serum resistance is a factor that plays a key role in the pathogenesis of B pertussis. We show that Czech recent isolates (2008-2015) are significantly more resistant to serum killing in vitro than the original vaccine strains (1954-1965). This phenomenon seems to result from the adaptation of global B. pertussis population to its human host. In addition, this diploma...
|
|
|
The structure and role of type III secretion system and other virulence factors in pathogenesis of pertussis
Štipl, Daniel ; Večerek, Branislav (advisor) ; Pinkas, Dominik (referee)
Bordetella pertussis is a significant human pathogen which colonises a respiratory tract. The infection with B. pertussis results in serious and highly contagious disease called pertussis or whooping cough. B. pertussis produces wide range of virulence factors such as pertussis toxin, adenylate cyclase toxin, dermonecrotic toxin, tracheal cytotoxin, adhesins and type III secretion system (T3SS). The BvgAS is two-component signal transduction system that provides the complex regulation of B. pertussis virulence. The virulence factor T3SS is used by some Gram-negative bacteria to colonise the host and is responsible for pathogenesis of the infection. T3SS takes a role in virulence of mammalian pathogen B. bronchiseptica, closely related to B. pertussis. The importance of T3SS in virulence of B. pertussis remains to be investigated. Significant advance in structure, function and regulation of the most of virulence factors have been accomplished in last few decades. The causative agents of pathogenesis in that infection remain unknown. Key words: Bordetella, T3SS, gene expresssion regulation, virulence factor, pathogenesis
|
guest ::
