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AHL genes in plant development
Škrabálková, Eliška ; Širl, Marek (advisor) ; Ortmannová, Jitka (referee)
Plant development is a complex process where different factors come into play. The expression of certain genes, which determine basic plant structure and its signaling pathways, is also of great importance. The AHL gene family, which in case of Arabidopsis thaliana includes 29 members, is one of those determinators that have an impact on plant development on several levels. As far as structure is concerned, the AHL proteins are typically composed of two parts - the DNA-bonding AT-hook motif and PPC domain, which defines nuclear localisation and eventually enables oligomerisation. In terms of phylogenetics, the AHL proteins are divided into clades A and B on the basis of number and type of these domains. When it comes to affecting plant development, these nuclear proteins are capable of bonding with the corresponding DNA, and in cooperation with other factors influencing gene expression. In the plant body the AHL proteins are involved in root and floral organogenesis and also cooperate with a number of signaling pathways of phytohormones, such as auxins, gibberellins, brassinosteroids or senescence hormones. Moreover, they take part in photomorphogenesis or control systematic immune responses of the plant. Keywords: AHL, AT-hook, PPC domain, nuclear protein, Arabidopsis thaliana
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Functional characterisation of AHL28 and its role in root system development
Škrabálková, Eliška ; Širl, Marek (advisor) ; Sabol, Peter (referee)
The root system architecture development is influenced by the interaction of numerous factors, one of which is the specific gene expression contributing to root development. The recently discovered AHL gene family, which, due to its characteristics act as transcriptional modulators, demonstrably comprises members taking part in regulation of root system development. Hereby we report the AHL28 gene that participates in this process. Its regulatory role corresponds with the expression pattern, regarding both the promotor activity and presence of nuclear localised protein. The expression can be observed in the cortex cells of transition zone of both primary and lateral roots. The phenothypic analysis of plants with modulated level of AHL28 transcription demonstrates its role in the primary root growth and differentiation, the apical meristem size and the number of lateral roots. Moreover, the gene also plays a role in vascular tissue patterning. Key words: AHL, nuclear protein, root system, apical meristem, lateral roots, xylem, Arabidopsis thaliana
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AHL genes in plant development
Škrabálková, Eliška ; Širl, Marek (advisor) ; Ortmannová, Jitka (referee)
Plant development is a complex process where different factors come into play. The expression of certain genes, which determine basic plant structure and its signaling pathways, is also of great importance. The AHL gene family, which in case of Arabidopsis thaliana includes 29 members, is one of those determinators that have an impact on plant development on several levels. As far as structure is concerned, the AHL proteins are typically composed of two parts - the DNA-bonding AT-hook motif and PPC domain, which defines nuclear localisation and eventually enables oligomerisation. In terms of phylogenetics, the AHL proteins are divided into clades A and B on the basis of number and type of these domains. When it comes to affecting plant development, these nuclear proteins are capable of bonding with the corresponding DNA, and in cooperation with other factors influencing gene expression. In the plant body the AHL proteins are involved in root and floral organogenesis and also cooperate with a number of signaling pathways of phytohormones, such as auxins, gibberellins, brassinosteroids or senescence hormones. Moreover, they take part in photomorphogenesis or control systematic immune responses of the plant. Keywords: AHL, AT-hook, PPC domain, nuclear protein, Arabidopsis thaliana
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