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Laboratory Diagnostics and HLA Typization of Patients with Rheumatoid Artritis.
Škoda, Marek ; Půtová, Ivana (advisor) ; Slavčev, Antonij (referee)
This work studies the relationship between DRB1 alleles and production of clinically most important autoantibodies in patients with rheumatoid arthritis (RA). A group of 81 patients with RA were diagnosed in immunological laboratory and genotyped. The prevalence of most often tested analytes were determined: antinuclear antibodies (ANA), rheumatoid factor (RF), anti-CCP antibodies and anti-MCV antibodies. HLA-DRB1 alleles were detected using PCR-SSP low resolution. The aim of this study was to determine the frequency of DRB1 alleles in Czech patients and to investigate the relationship between DRB1 alleles and production of particular antibodies. The frequency of HLA-DRB1*01 and HLA-DRB1*04 alleles was significantly increased in RA patients compared to healthy subjects. In contrast, the frequency of DRB1*15 allele was significantly reduced. Studying the relationship between DRB1 alleles and presence of antibodies showed a significantly increased frequency of DRB1*04 allele in patients with positive ACPA antibodies (anti-CCP, anti-MCV). Regarding other antibodies (ANA, RF), no relationship between their production and presence of DRB1 alleles was found. Comparison of anti-CCP and anti-MCV antibodies levels between groups of RA patients with the presence and absence of DRB1*04 alleles showed no difference....
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Laboratory Diagnostics and HLA Typization of Patients with Rheumatoid Artritis.
Škoda, Marek ; Půtová, Ivana (advisor) ; Slavčev, Antonij (referee)
This work studies the relationship between DRB1 alleles and production of clinically most important autoantibodies in patients with rheumatoid arthritis (RA). A group of 81 patients with RA were diagnosed in immunological laboratory and genotyped. The prevalence of most often tested analytes were determined: antinuclear antibodies (ANA), rheumatoid factor (RF), anti-CCP antibodies and anti-MCV antibodies. HLA-DRB1 alleles were detected using PCR-SSP low resolution. The aim of this study was to determine the frequency of DRB1 alleles in Czech patients and to investigate the relationship between DRB1 alleles and production of particular antibodies. The frequency of HLA-DRB1*01 and HLA-DRB1*04 alleles was significantly increased in RA patients compared to healthy subjects. In contrast, the frequency of DRB1*15 allele was significantly reduced. Studying the relationship between DRB1 alleles and presence of antibodies showed a significantly increased frequency of DRB1*04 allele in patients with positive ACPA antibodies (anti-CCP, anti-MCV). Regarding other antibodies (ANA, RF), no relationship between their production and presence of DRB1 alleles was found. Comparison of anti-CCP and anti-MCV antibodies levels between groups of RA patients with the presence and absence of DRB1*04 alleles showed no difference....
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Research of influence of degradation process on changes of mechanical properties of polymeric particle composites
Škoda, Marek ; Müller, Miroslav (advisor) ; Hrabě, Petr (referee)
This thesis is engaged in research of influence of degradation processes on changes of mechanical properties of polymeric particle composites. In the first part, literal research, are listed basic informations about composite materials, their history, definition, distribution and utilization. It further describes the procedures that should be followed in designing bonded joints, and summarize the advantages and disadvantages of adhesive bonding technology. Subsequently, the literature research is dealing with a corrosion and its basic division. At the end of the first part of a thesis are informations about the types of
stress on bonded joints.
In a second part of a thesis is analyzed the procedure of the experiment itself. Its objective is to monitor the process of degradation, which acts on a bonded joints with different kinds of additives depending on time. As a degradation medium was chosen solution of utility water and road salt.
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