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MALDI - TOF MS identification of filamentous fungi.
ŠNAJDROVÁ, Martina
The method of mass spectrometry with laser desorption / ionization time of flight in the presence of the matrix analyzer ( MALDI-TOF MS ) is using a technique called soft ionization. This is a fast and ever-evolving method, which has found application for the analysis of proteins, glycoproteins, nucleic acids, lipids and high molecular weight and low molecular weight substances. This method has brought inovation to the routine microbiology laboratories. MALDI-TOF mass spectrometry can be used for identification aerobic and anaerobic bacteria, yeast, mycobacteria, but also to determine the genus and species of filamentous fungi. Except identification of microorganisms, is possible to detect the mechanisms of antibiotic resistance in bacteria (e.g., - lactamases ability for the inactivation of - lactam antibiotics ). MALDI TOF MS is an easy to use method more accurate, faster and more cost efficient than commonly used biochemical tests and comparing macroscopic and microscopic appearance. Mass spectrometry MALDI TOF generate mass spectra of proteins (bacteria) and glycoproteins (fungi), which are species -specific for identification of strain. Identification of the strain by comparing the obtained mass spectra with reference spectra databases . In the period from January 2013 to February 2014 were in the practical part examined 114 isolates of filamentous fungi, 80 strains as agent of a superficial mycoses and 34 strains as agents of systemic and organ fungal infections. All 114 samples were determined by conventional methods and also were identified by MALDI TOF MS. The selected group of isolates was compared to molecular genetic methods (PCR) followed by the sequence of the amplicon. MALDI TOF mass spectrometry is a method for easily identifying bacteria. During testing of filamentous fungi is a main importance on the stage of preparation of the sample before testing. It was tried several workflows whose success is summarized in this thesis.

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4 Šnajdrová, Marie
4 Šnajdrová, Michaela
1 Šnajdrová, Miluše
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