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Determination of platelet activation markers expression in the pathophysiology of thrombotic states
Řádek, Martin
Background/Aims: Thrombophilia, ie an increased predisposition to venous and arterial thrombosis, is a complex disease caused by disorders of platelets and endothelial, among others. Circulating endothelial cells (CEC) and endothelial progenitor cells (EPC) have been described as markers of endothelial damage and dysfunction, respectively as markers of its recovery in many diseases, including thrombotic complications. However, their significance in patients with known thrombophilia has not yet been investigated. Both CEC and EPC represent extremely rare cell populations found in peripheral blood. Therefore, it is essential to use exclusively standardized and sensitive methods for their identification and quantification. The aim of the study was to identify and quantify CEC and EPC in the peripheral blood of patients with congenital thrombophilia and to evaluate their importance as markers of endothelial and platelet activity in context with the risk of thrombosis occuring and recurrence. Methods: Analysis of the number of CEC and EPC in the peripheral blood of patients with thrombophilia with or without a history of thrombosis and patients with acute thrombosis was performed by multicolor flow cytometry. The CEC and EPC reference values were determined on a group of healthy controls. Patients with...
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Determination of platelet activation markers expression in the pathophysiology of thrombotic states
Řádek, Martin ; Kvasnička, Tomáš (advisor) ; Dulíček, Petr (referee) ; Říhová, Lucie (referee)
Background/Aims: Thrombophilia, ie an increased predisposition to venous and arterial thrombosis, is a complex disease caused by disorders of platelets and endothelial, among others. Circulating endothelial cells (CEC) and endothelial progenitor cells (EPC) have been described as markers of endothelial damage and dysfunction, respectively as markers of its recovery in many diseases, including thrombotic complications. However, their significance in patients with known thrombophilia has not yet been investigated. Both CEC and EPC represent extremely rare cell populations found in peripheral blood. Therefore, it is essential to use exclusively standardized and sensitive methods for their identification and quantification. The aim of the study was to identify and quantify CEC and EPC in the peripheral blood of patients with congenital thrombophilia and to evaluate their importance as markers of endothelial and platelet activity in context with the risk of thrombosis occuring and recurrence. Methods: Analysis of the number of CEC and EPC in the peripheral blood of patients with thrombophilia with or without a history of thrombosis and patients with acute thrombosis was performed by multicolor flow cytometry. The CEC and EPC reference values were determined on a group of healthy controls. Patients with...
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Isolation and characterization of photosynthetic complexes from photoheterotrophic bacteria
Řádek, Martin ; Wilhelmová, Naďa (referee) ; Kučera, Tomáš (advisor)
Aerobic anoxygenic phototrophs (AAPs) represent a relatively recently discovered unique BChl a-containing bacterial community. These organisms own a fully functional bacterial photosynthetic system. Their photosynthesis is operative only in the presence of oxygen. However, they are not able to grow autotrophically, they require a source of organic carbon for growth and metabolism. I have characterized the photosynthetic apparatus of Erythrobacter sp. NAP1, Roseobacter sp. COL2P and the strains Dor-2m, Dor-vul and Zun-kholbo. BChl a performs the function of the main light-harvesting pigment. Spheroidenone is the major carotenoid of the strains COL2P, Dor-2m, Dor-vul and Zun-kholbo. The NAP1 strain contains "non-photosynthetic" erythroxanthin sulfate, which is bound neither to the reaction center nor to the light-harvesting complex. Bacteriorubixanthinal, zeaxanthin and erythroxanthin are the main photosynthetic carotenoids of NAP1, coupled to the light- harvesting system. This system is composed of inner LH1 complex and reaction center, with no peripheral LH2 complexes. The photosynthetic unit size, in comparison with a typical anaerobic phototroph Rhodobacter sphaeroides, is limited due to the absence of LH2 antenna. The purified LH1-RC complex isolated from NAP1 is relatively thermostable,...
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