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Medical cannabis at the pharmacy
Černý, Ondřej ; Kostřiba, Jan (advisor) ; Smejkalová, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Social and Clinical Pharmacy Candidate: Mgr. Ondřej Černý Supervisor: PharmDr. Jan Kostřiba, Ph.D. Title of thesis: Medical cannabis at the pharmacy Key words: cannabinoids, individual preparation, medical cannabis in the Czech Republic This rigorous thesis is focused on the medical cannabis (MC). The aim of the thesis was first to familiarise the reader with the description of the substances contained in the herbal cannabis and its effects on the human body. Furthermore, state the current position of MC in the Czech Republic. The aim of the experimental part was to present aspects of the implementation of MC treatment at the pharmacy based on the interpretation of legislation. Furthermore, to statistically evaluate the development of this treatment within the hospital pharmacy in České Budějovice. Records of MC dispensed from the register book of addictive substances from 2019 to 2020 were used as a basis for this statistical analysis. The aim was also to evaluate the development of MC treatment throughout the Czech Republic. The data used in this thesis were published on the websites of the State Agency for Medical Cannabis (SAKL) and the State Institute for Drug Control (SÚKL) from 2015 to 2020. The results of the thesis...
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Development of algorithmic thinking in upper secondary education using programmable robotic systems
Černý, Ondřej ; Jeřábek, Tomáš (advisor) ; Štípek, Jiří (referee)
Obsah Použité zkratky ...................................................................................................................... 9 Úvod .................................................................................................................................... 10 Výzkumný problém a cíle práce...................................................................................... 11 Využité metody práce...................................................................................................... 12 1 Teoretická východiska................................................................................................. 13 1.1 Informatické myšlení ............................................................................................ 14 1.2 Algoritmické myšlení ........................................................................................... 18 1.2.1 Algoritmus..................................................................................................... 19 1.2.2 Vlastnosti algoritmů ...................................................................................... 20 1.2.3 Způsoby zápisu algoritmů ............................................................................. 22 1.2.4 Základní algoritmické konstrukce...
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Heterogeneity of expression of virulence factors of Salmonella.
Mathéová, Paulína ; Černý, Ondřej (advisor) ; Lichá, Irena (referee)
The emergence of phenotypically heterogeneous individuals within an isogenic bacterial population is considered to be an important adaptation to the host environment. It allows survival of some bacterial subpopulations under diverse stress conditions caused by the host immune system, the emergence of a "division of labor" and cooperation between individual bacteria. Bacteria of the genus Salmonella are important pathogens in humans and livestock. Many of the key virulence factors of Salmonella are heterogeneously expressed. The phenotypic diversity of individual bacteria allows certain individuals to escape the host's immune system and ensure that the gene pool is preserved to future generations. In case when change in conditions causes complete extinction of part of the Salmonella population from the environment, the remaining individuals are able to restore the size of the population and phenotypic diversity, after overcoming unfavourable conditions. This work summarises the knowledge about heterogeneity of expression of virulence factors of Salmonella and the characteristics of individual subpopulations in different environmental conditions. Keywords: Salmonella, heterogeneity, virulence factors, bet-hedging, division-of-labor, bacterial subpopulations.
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Regulation of virulence factors of Staphylococcus aureus
Šaňková, Michaela ; Lichá, Irena (advisor) ; Černý, Ondřej (referee)
Staphylococcus aureus is a gram-positive pathogenic bacterium that regulates virulence factors production in response to changing environmental conditions. S. aureus cells evolved a complex regulatory network, including a number of regulatory proteins, transcriptional factors and two- component systems. One of the most important S. aureus regulatory systems is the Agr system (Accessory gene regulator) that perceives its own population density by sensing a "quorum-sensing" signal in a form of autoinducing peptid (AIP). Agr system encodes a global regulatory RNAIII that regulates the expression of target virulence factors, which includes surface proteins as well as extracellular toxins and enzymes. The family of global protein regulators SarA and transcriptional sigma factor B also play a significant role in the regulation of S. aureus virulence. The production of virulence factors is also regulated in response to specific signals from extracellular environment by two- component-systems, which includes the regulator of exoprotein production SaeRS, the regulator of autolysis ArlRS and the regulator of respiratory response SrrAB. Key words: Staphylococcus aureus, virulence factors, Agr, quorum-sensing, RNAIII, SarA, SigB
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Signaling effects of adenylate cyclase toxin action on phagocytes
Černý, Ondřej
The adenylate cyclase toxin (CyaA) plays a key role in the virulence of Bordetella pertussis. CyaA penetrates CR3-expressing phagocytes and catalyzes the uncontrolled conversion of cytosolic ATP to the key second messenger molecule cAMP. This paralyzes the capacity of neutrophils and macrophages to kill bacteria by oxidative burst and opsonophagocytic mechanisms. Here we show that CyaA suppresses the production of bactericidal reactive oxygen and nitrogen species in neutrophils and macrophages, respectively. The inhibition of reactive oxygen species (ROS) production is most-likely achieved by the combined PKA-dependent inhibition of PLC and Epac-dependent dysregulation of NADPH oxidase assembly. Activation of PKA or Epac interfered with fMLP-induced ROS production and the inhibition of PKA partially reversed the CyaA-mediated inhibition of ROS production. CyaA/cAMP signaling then inhibited DAG formation, while the PIP3 formation was not influenced. These results suggest that cAMP produced by CyaA influences the composition of target membranes. We further show here that cAMP signaling through the PKA pathway activates the tyrosine phosphatase SHP-1 and suppresses the production of reactive nitrogen species (RNS) in macrophages. Selective activation of PKA interfered with LPS- induced iNOS expression...
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Film Festival Finále Plzeň
Černý, Ondřej ; Klimeš, Ivan (advisor) ; Bláhová, Jindřiška (referee)
This thesis is devoted to the film festival Finále Plzeň which showcases to its visitors every spring a complex summary of Czech cinema. Even though the festival's main objective are new inland movies and TV shows, which are presented and awarded within special competitive sections, in its accompanying programme there are countless noncompeting archive and foreign films. This is due to the festival's dramaturgy that has been completely overhauled and helped with the festival's integration into European film festival net. The main focus of this paper is the current reimagination of this festival under the new management that organizes this event since the year 2016 and aims towards a complete renaissance of this traditional film event.
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Assessing the effectiveness of invested public resources to ensure the quality and availability of public cultural needs
Dostál, Petr ; Černý, Ondřej ; Daněk, Petr ; Drobný, Tomáš ; Gregorini, Jindřich ; Richter, Vít ; Gregoriniová, Jindřiška ; Lázňovská, Lenka ; Přílepková, Michaela ; Vávrová, Barbora ; Vítová, Kateřina
Metodika je v prvním plánu určena správním orgánům v resortu kultury, které v rámci státní kulturní politiky zodpovídají za podporu rozvoje jednotlivých kulturních aktivit a organizací. Jde jednak o orgány státní správy a útvary krajských a městských samospráv, které jsou pověřeny praktickou realizací kulturní politiky, zpracovávají strategické rozvojové dokumenty a doporučení pro orgány místních samospráv na úrovni obcí (proto je jednou z významných částí dokumentu kapitola Zřizovatelé – poskytovatelé veřejné podpory). Metodika má dále sloužit potřebám klíčových veřejnoprávních a soukromých institucí činných v sektoru kultury – jak významných neziskových organizací, tak i soukromých subjektů – aukčních síní, galerií, divadelních souborů i hudebních těles. V druhém plánu má metodika sloužit vědeckým a výzkumným subjektům veřejnoprávního i soukromého charakteru, jak subjektům úzce zaměřeným na výzkum kultury tak i organizacím zaměřeným na řešení širších ekonomických souvislostí a dopadů kultury. Jde jak o výzkum kreativních odvětví, tak i o specifická vědecká zadání v jednotlivých ekonomických odvětvích (cestovní ruch, export kulturní produkce, mezinárodní kulturní spolupráce apod.).
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Comparing approaches to improving processes in selected banks
Voříšková, Kristýna ; Svobodová, Hana (advisor) ; Černý, Ondřej (referee)
Diploma thesis focuses on individual approaches to increase process efficiency in Czech banks. Target banks are Komerční banka, Česká spořitelna and Raiffeisenbank. Trend of bank services commoditization is forcing banks to reduce products prices and increase quality. The goal of this thesis is to compare approaches of forementioned banks and evaluate them based on pre-set criteria. After the analysis suggestions will be made. The first part diploma thesis explains process management terminology and presents specific theoretical approaches to process enhancing. The second part compares specific projects in three banks and suggests specific improvements. Diploma thesis also confirms the philosophy of continuous process improvement is valid for tertiary sector as well.
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Stability indicating HPLC method for evaluation of fluoxetine
Černý, Ondřej ; Kastner, Petr (advisor) ; Pilařová, Pavla (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Candidate: Ondřej Černý Supervisor: PharmDr. Petr Kastner, Ph.D. Title of thesis: Stability inducing HPLC method for evaluation of fluoxetine The purpose of this thesis was to develop and validate the stability - indicating HPLC method for evaluating Fluoxetine-hydrochloride. Fluoxetine-hydrochloride is a drug from the SSRI group used to treat depression of various etiologies. The basic method was adopted from the Czech Pharmacopoeia 2009. The given Pharmacopoeia method is not sufficient, as the peak of the degradation product Fluoxetine-hydrochloride interfered in the so - called dead retention time of the used column. Its quantitative determination was impossible for this reason. Development of the method consisted in adjustment of the mobile phase using gradient elution. The mobile phase was composed of a mixture of methanol, tetrahydrofuran and triethylamine solution, and it was divided into two containers. The container A consisted of the mixture (MeOH : THF) : TEA solution - 70: 30 (v/v) and the container B consisted of the mixture (MeOH : THF) : TEA solution - 5: 95 (v/v). The mixture (MeOH : THF) was prepared in the ratio of 8 : 30 (v/v). The chromatographic conditions...
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