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Histopathologic and molecular diagnostics of lung and pleural neoplasms
Čapková, Linda ; Kodet, Roman (advisor) ; Matěj, Radoslav (referee) ; Skřičková, Jana (referee)
The aim of the presented thesis is to introduce modern molecular methods that may contribute to deepening our knowledge of pulmonary carcinogenesis. Furthermore, we would like to point out the significance of immunohistochemistry in differential diagnostics of the most common primary pulmonary and pleural neoplasms. Lung carcinoma of all histological subtypes is a result of stepwise accumulation of genetic and epigenetic changes, including allelic losses (LOH), chromosomal instability and imbalance, oncogene and tumor suppressor gene mutations, epigenetic silencing by promotor hypermethylation or aberrant gene expression guidinig cell proliferation. In our study, certain genetic changes were detected in squamous cell carcinomas (SCC) of the lung, their precancerous lesions as well as in normal bronchial mucosa in a selected group of heavy smokers with limited ventilation. We investigated the relation between molecular changes of LOH type in chromosomal regions harbouring mismatch repair genes and cell cycle regulators and human telomerase reverse transcriptase (hTERT) mRNA expression. We also evaluated the relation between these molecular changes and histologic pattern in bronchial mucosa in this patient group. hTERT mRNA expression analysis in correlation with LOH in bronchial mucosa of heavy...
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