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Role of two lysine amino acid residues in transmembrane regions in agonist recognition of the human P2Y12 receptor
Ćwierz, Jan ; Pávek, Petr (advisor) ; Šimůnek, Tomáš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Jan Ćwierz Supervisors: Prof. Dr. med. Ivar von Kügelgen Doc. PharmDr. Petr Pávek, Ph.D. Title of diploma thesis: Role of two lysine amino acid residues in transmembrane regions in agonist recognition of the human P2Y12 receptor Human P2Y12 receptor expressed on platelets plays a key role in their physiological function. It importantly participates in maintenance of normal haemostasis and is often targeted by antiplatelet medication, such as clopidogrel. Replacement of amino acid residues responsible for ADP binding, which is a naturally occurring negatively charged agonist, can lead to impaired ligand-receptor interaction. Aim of this work was to study the role of two lysine amino acid residues in the position 173 and 174 of the P2Y12 receptor protein and subsequent changes in 2-methylthio-ADP binding. Two positively charged lysine amino acid residues were substituted with neutral residue alanine. Receptor expression in Chinese hamster ovary was analyzed by immunofluorescence staining and laser scanning confocal microscopy. Subsequently, the wild-type receptor and three mutant variants (K173A, K174A and K173A/K174A) were examined by means of 2-methylthio-ADP-induced responses in...
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Role of two lysine amino acid residues in transmembrane regions in agonist recognition of the human P2Y12 receptor
Ćwierz, Jan ; Pávek, Petr (advisor) ; Šimůnek, Tomáš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Jan Ćwierz Supervisors: Prof. Dr. med. Ivar von Kügelgen Doc. PharmDr. Petr Pávek, Ph.D. Title of diploma thesis: Role of two lysine amino acid residues in transmembrane regions in agonist recognition of the human P2Y12 receptor Human P2Y12 receptor expressed on platelets plays a key role in their physiological function. It importantly participates in maintenance of normal haemostasis and is often targeted by antiplatelet medication, such as clopidogrel. Replacement of amino acid residues responsible for ADP binding, which is a naturally occurring negatively charged agonist, can lead to impaired ligand-receptor interaction. Aim of this work was to study the role of two lysine amino acid residues in the position 173 and 174 of the P2Y12 receptor protein and subsequent changes in 2-methylthio-ADP binding. Two positively charged lysine amino acid residues were substituted with neutral residue alanine. Receptor expression in Chinese hamster ovary was analyzed by immunofluorescence staining and laser scanning confocal microscopy. Subsequently, the wild-type receptor and three mutant variants (K173A, K174A and K173A/K174A) were examined by means of 2-methylthio-ADP-induced responses in...
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Application of the Balanced Scorecard in the company ELLA-CS
Cwierz, Jan ; Neumaierová, Inka (advisor) ; Beneš, Oldřich (referee)
Balanced Scorecard belongs to the most prominent managerial tools for performance measurement and strategy management. Aim of this thesis is to describe BSC with emphasis to small and medium enterprises using literature review. Additional supporting tools for strategy formulation are included as well. BSC is then proposed for implementation in privately owned small enterprise in the sector of medical devices. Based on the thorough analysis of the company and its environmental factors operational and strategical performance gaps are identified. Such gaps are then addressed by means of BSC including financial, customer, internal processes and learning and growth perspective. Despite some limitations inherent to SMEs in general introduction of BSC is feasible and could be of value to all company stakeholders.
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