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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Modulace intracelulární kaspázové mašinérie s využitím explantátových kultur
 Matalová, Eva ; Fleischmannová, Jana ; Norek, Adam ; Míšek, Ivan
Organ explant cultures eliminate animal suffering during experiments but simultaneously allow investigations of intact organ, tissue and cell systems with preserved interactions corresponding to the situation in vivo. Moreover, the explants can be manipulated in several ways using modern micromanipulation techniques (e.g. electroporation, inhibition). We show possible usage of explant culture approaches in embryonal apoptosis research in three models – mouse limb digitalization, tooth germ morphogenesis, separation of middle ear ossicles. Data from specific pharmaceutical inhibitions focused on key apoptotic molecules – caspases - are demonstrated.
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Molekulární aspekty hypodoncie
 Fleischmannová, Jana ; Krejčí, P. ; Matalová, Eva ; Míšek, Ivan
Numeric dental anomalies are the most common craniofacial congenital malformations in humans. More than 20 % of human population miss one or more third molars, approximately 5 % of population display agenesis of another tooth. In this contribution molecular events underlying tooth formation (more than 200 genes have been identified so far) and defects in tooth germ formation are correlated. Both syndromic forms of hypodonia (e.g. Rieger syndrome, anhidrotic ectodermal dysplasia) and non-syndromic forms (related to pax9, msx1, axin2) were investigated.
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