National Repository of Grey Literature 729 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Mammalian proteins carrying zinc finger arrays
Vobruba, Adam ; Svoboda, Petr (advisor) ; Mašek, Jan (referee)
A zinc finger is a small peptide motif stabilised by a single zinc ion, best known for its capability to specifically bind a 3-nucleotide sequence of DNA, depending on the exact amino acids present in the DNA-binding positions. Zinc fingers are unique in their ability to freely link together and form longer tandem arrays, which can bind DNA targets of any length and sequence determined by the combination of individual fingers. These arrays can easily mutate and be rebuilt to change binding specificity, allowing great flexibility and helping zinc fingers to their widespread presence in numerous endogenous proteins of various functions. This property of zinc finger arrays also made them a suitable tool for the creation of custom DNA- binding domains for genetic engineering. This thesis provides an overview of the discovery, structure and function of these domains and then reviews and discusses selected naturally occurring mammalian zinc finger proteins and their properties, showcasing diverse uses zinc finger arrays have been adapted for throughout evolution. The history and future of zinc fingers in artificial proteins created for gene therapy and research are discussed as well. Keywords: zinc finger, ZnF, KRAB, KZFP, CTCF, PRDM9, ZFN, mammals
Manufacturing of the electric motor cover
Kolář, Zdeněk ; Svoboda, Petr (referee) ; Císařová, Michaela (advisor)
The bachelor 's thesis focuses on the design of the production of an electric motoro cover made prom low-carbon steel 11 305 with a thicnkess of 1,5 mm. For an annual production series of 100 000 pieces per year, the technology of deep drawing without wall thinning has been selected. In the practical part, it was found that the cover body can be produced in a single drawing operation. The blank diameter, increased by the trimming allowance, is 212 mm, which will be produced by cutting from coils with a width of 222 mm and a outer diameter of 1200 mm. Total fore of 249,18 kN includes the drawing component, holder and ejector. Production is carried out on the CUPJ 63 DEU hydraulic press from HŠV Polička, equipped with a lower hydraulic device. Deep drawing die is designed for the machine, which enables the production of the cover body in one drawing operation. Design is supplemented with a detailed assembly procedure. Lubricant MORLAUB KFP 61 V is used for optimal process condition.
Design of a single-purpose clamping device for mass production
Okénka, Jan ; Michalec, Michal (referee) ; Svoboda, Petr (advisor)
Bachelor thesis deals with the problem of clamping of a complex cast part on a four-axis CNC machine in serial production. The aim was to design a clamping jig to reduce the total number of clamping operations required. A total of 4 concepts were presented and the best design was selected according to the criteria. The proposed solution is a hydraulic centering vise, equipped with a center piece to locate two workpieces, and two rotating jaws that adjust by the workpiece. A series of 250,000 pieces per year can be fulfilled with this fixture. The jig can be easily converted to another type of production by changing the jaws and the middle part. The necessary clamping force for secure clamping was also calculated and it was verified by finite element analysis that no permanent deformation of the workpiece would occur during clamping. The work also includes a review of commercially available jigs, aluminium alloys for die casting and selected CNC milling machines.
Design of Rubik's Cube Solver
Palán, Petr ; Šperka, Petr (referee) ; Svoboda, Petr (advisor)
The Rubik’s Cube is one of the most famous puzzles in the world, it has evolved into countless variations and in its solving is competed all over the world. However, the record time for its solution is no longer held by a human, but by a robot. This bachelor thesis deals with the structural design and subsequent implementation of the mechanical part of the solver for the Rubik's Cube. A research of existing solvers is carried out and then individual conceptual designs are proposed. The chosen variant is then elaborated in the structural design. It individually controls 5 walls of the cube and thus ensures the possibility of its solving without the need for further positioning and at the same time there is space for comfortable insertion of the cube. The design is then realised by 3D printing. Thereafter an electronic part is connected to the created structure and a simple program written to test the structure is executed using a microcomputer. The result of the testing is that the design is functional but there is room for improvement.
Design of a fixture for machining a car air conditioning component
Šetek, Tomáš ; Jeniš, Filip (referee) ; Svoboda, Petr (advisor)
The bachelor thesis deals with the design of the fixture. Clamping jigs enable fast and high quality production of components in the ever increasing demand of machined blanks on CNC machining centres. The aim of this thesis was to design a milling jig for an automotive compressor component with respect to minimum manufacturing cost, simplicity of design and reduction in machine time. The output of the work is the selected design prepared in the form of drawings. The work includes a review of design principles in jig design, commercially available fixtures, CNC milling machines and rotary or tilting tables that serve as additional axes of CNC milling machines.
Possibilities of Forming Limits Determination
Gímeš, Martin ; Svoboda, Petr (referee) ; Řiháček, Jan (advisor)
The bachelor thesis describes selected technological tests for evaluating formability. The thesis is divided into two main parts, which deals with the evaluation of sheet and bulk formability of materials. Based on a literature review, the thesis presents the factors influencing the formability and material plasticity. Furthermore, it discusses the principles of selected tests. Subsequently, it addresses the conditions and procedures set by standards and the necessity to adhere to them. Possible test specimens and their modifications are considered. The number of repeated tests, and possibly the evaluation of suitability or unsuitability of deformed samples for formability assessment, are discussed. Methods of evaluation for individual tests are outlined. The thesis presents experimental results for each test with descriptions of the test specimens and materials used, obtained from practical sources.
Optimization of infill selection with an emphasis on maximum dynamic resistance
Konvalina, Michal ; Svoboda, Petr (referee) ; Jopek, Miroslav (advisor)
The thesis deals with the study of the effect of different wall thicknesses of 3D printed infill patterns at constant cross-sectional area on their final properties under static and dynamic loading. Based on literature study, two experimental methods are chosen. The quasi-static test and the Taylor anvil test. For the experiment, square and hexagonal grid specimens are printed using FDM technology from PLA material. Quasi-static test is used to measure the static properties such as maximum compressive strength, yield stress, modulus of elasticity and to derive material model of Johnson-Cook. The dynamic impact test TAT is used to evaluate different types of patterns at high impact velocities. In ANSYS, the specimens that resisted the highest impact velocities in TAT are simulated and strain rate sensitivity parameter is optimized for the specific velocity. The highest dynamic resistance was achieved by a square infill pattern with wall thickness of 0,4 mm at an impact velocity of 37 ms-1.
Modification of a multi-recess hydrostatic bearing pad geometry
Šimara, Vít ; Robenek, Dětřich (referee) ; Svoboda, Petr (advisor)
In the design of a hydrostatic bearing, basic analytical expressions assuming parallelism of sliding surfaces and simple geometry can be employed. In this study, the properties of a specific pocket shape were investigated even under conditions of tilt, which can arise from assembly errors or asymmetric loading. Based on experimental data, a Computational Fluid Dynamics (CFD) model of hydrostatic lubrication has been developed and validated. A constant flow through all recesses was considered and conditions of no movement of the sliding surface were assumed. The model was subsequently modified to a variant with discharge grooves separating individual recesses and a variant with variable recess position relative to the centre of the pocket. The results demonstrated that the pocket's ability to carry asymmetric loading increases mainly with increasing distance of the recess from the centre, followed by their size. However, this ability comes at the cost of higher performance losses in hydrostatic lubrication. The results of this work can be used for the design of pockets with different methods of pocket support or alignment.
Authorization and building permit
Charvátová, Veronika ; Franková, Martina (advisor) ; Svoboda, Petr (referee)
The diploma thesis entitled Authorization and building permit deals with two basic approaches, authorization and permission to build. The aim of this work is to analyze the legal regulation of permits and building permits, with regard to the recodification of construction law. Given that this is a very current topic, it is therefore possible to bring new insight into this area as a result of the development of legislation. The work is divided into four separated logically connected parts. First, basic concepts relevant to the topic of the work are introduced. These are mainly the concepts of land and building, which are expressed in the context of various legal regulations. These are mostly delineations according to the Civil Code, the Cadastral Act and the Construction Act. The second part deals with the legal definition of the private right to build. In this part, private law titles are defined in particular and there is an effort to assess suitability for the implementation of the plan. The question of ownership is also addressed here, as a title entitling to construction. The acquisition of ownership rights and it is possible limitations are mentioned here. The issue of building permits is followed by another part, which is already under public law and deals with the issue of building permits....
Voluntary instruments for environmental protection
Kolářová, Karolína ; Fabšíková, Tereza (advisor) ; Svoboda, Petr (referee)
Voluntary instruments for environmental protection Abstract This diploma thesis focuses on voluntary instruments for environmental protection. The main aim is to provide a comprehensive overview of the most significant voluntary instruments, characterize them, demonstrate their practical applications, and analyse their legal regulation at the national, European, or international level. To adequately introduce the topic, the first part of this thesis is dedicated to environmental protection instruments in general. The second part delves into a group of voluntary instruments, providing a detailed description and historical context of their emergence within environmental policy. Subsequent sections of this thesis focus on selected voluntary instruments, categorized according to their assessed legal and practical relevance, for closer examination. The third part initially addresses voluntary environmental agreements (also known as agreements on protective conditions), which represent a relatively well-known legal concept in the national context and are regulated to varying extents by Law No. 114/1992 Coll., on Nature and Landscape Protection, as amended. The fourth part subsequently analyses two formalized environmental management systems, specifically EMS implemented according to the international standard ISO...

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