National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Methodology for detection identification of fungi responsible for the cereal contamination with ochratoxin A and citrinin using PCR: Methodology for practice
Salava, Jaroslav ; Novotný, David
The aim of this methodology was to describe a protocol for the rapid and reliable detection of microscopic fungi from the genusPenicillium and Aspergillus capable of producing mycotoxins ochratoxin A and citrinin in cereals, preferably prior to their production and accumulation, using polymerase chain reaction (PCR) and their use, e.g. for the evaluation of health and hygienic safety of plant products.
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Methodology for the phenotyping resistance to Plum pox virus in peach
Salava, Jaroslav ; Polák, Jaroslav
Sharka disease, which is caused by the Plum pox virus (PPV), is the most economically important virus disease in fruit trees worldwide. Since it is not possible to completely eradicate PPV in the Czech Republic, it is necessary to find another way to control this pathogen. The most promising seems to be the breeding of new cultivars for resistance. This methodology provides a comprehensive protocol for testing PPV resistance in peach. It is based on visual assessment of disease symptoms on leaves and fruits, and determining the presence of the virus in plants using ELISA and RT-PCR techniques.
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