National Repository of Grey Literature 82 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Effect of Type 2 diabetes on the mouse reproductive parametres
Stiborová, Martina ; Pěknicová, Jana (advisor) ; Nagyová, Eva (referee)
Infertility is defined as an inability to conceive a child within one year of regular sexual intercourse. It affects up to 15 % of couples worldwide (WHO, 2010). The male factor contributes to the total infertility with more than 50 %. Fertility of a man is influenced by several factors such as genetic background, environment and various diseases such as diabetes mellitus (DM). Diabetes mellitus is a serious health problem that affects 451 million people worldwide (18-99 years) and the number of people with this disease still increases (Cho a spol., 2018). In addition, parenthood is postponed to middle age when the fertility decreases and metabolic diseases such as type 2 diabetes mellitus (DM2) appear. The aim of this thesis was to investigate the effect of type 2 diabetes on reproductive parameters of mouse inbred line C57BL/6J compared to the control group and the possible effect of paternal diabetes on the first filial generation. In the evaluation of the effect of DM2 on reproductive parameters, we used innovative methods to study internal state of sperm and testes. Results of our work showed that DM2 influenced the weight of body, prostate and liver. The weight of testes, epididymis and liver was reduced in the offspring. Furthermore, sperm morphology and intraacrosomal protein status were...
Protein synthesis and protein degradation in mammalian oocyte development
Šušor, Andrej ; Motlík, Jan (advisor) ; Pěknicová, Jana (referee) ; Petr, Jaroslav (referee)
Concr,usroNs We anďysed eucaryoticinitiation t,anslation factorsthat are responsiblefor protein qmthesisin mammalianoocýes. As well we examinedthe affinity.of this factorsto.'mCap, Sepharoseduring in útro conďtion...Inour'laboratorywas optimisedthe protocol for generation of poreine parttrenotesthat serve us as a model for anďysis of. cellular processos in .rygotes with biochemical approaches.. : , , - Antibod}r micÍoanay analysis was used to investigate the: regulation of signalling pathways primarily during meiotic maturationof pig oocytesand subsequentlyin comparisonto other species(bovine,frog andseastar). on the base of proteomeanďysis of maturationof oocýes was chosenUCH-LI molebuleandis studiedin detail in mammalian oocyte. Abnost thorougily was analysed function of UCH-LI in porcine and bovine oocyte using specific inhibitors and overexpression. The mechanism that is involved UCH-LI in antipolyspermydefencein mammalian oocyte was unveiled in our laboratory.
Recombinant Fragments of Antibodies
Král, Vlastimil ; Sedláček, Juraj (advisor) ; Pěknicová, Jana (referee) ; Hašek, Jindřich (referee)
6. CoNct usloN The aim of the thesis was to establish, in the national conditions, technology of preparation of antibody reconrbinant fragrnents and to verífo the cornplete procedure using several model monoclonal antibodies with a potential diagnostic and therapeutic use. For tfuee antibodies' mAb TU-20, M75 and MEM97, recombinant |Ťagments in various formats (scFv fragments monovalent and bivalent, i.e. diabody, and intrabody for intracellular expression) were constructed and for their heterologous expression, vectors allowing expression in E. coli (as cytoplasmic inclusions, periplasmic inclusions and in soluble form) and in Drosophila 32 cells (expression of glycosylated forms of scFv fragments into the medium) were used. In case of proteins expressed in irrsoluble form, especially scFv F|1.2.32, renaturation procedures to obtain active scFv fragments were developed and optimized. The efnect of the length of the linker -(GlyrSer).- (where x is I to 4) connecting the variable domains of the light and heavy chain on the formation of different multimeric forms of scFv rvas studied. For obtaining solell monomeric scFv fragment, the length of 20 amino acid residues tumsd out optimal. Fragnrents rvith a linker l5 residues long. formed a mixture of monomers, dimers and trimers. the proportion of rvhich rvas...
The Role of Energetic Metabolism in the t-haplotype Transmission ratio distortion
Marvanová, Hana ; Paulasová, Petra (advisor) ; Pěknicová, Jana (referee)
When two alleles carried by a heterozygote are transmitted unequally to the zygote at the time of fertilization, transmission ratio distortion occurs. The best studied example of this phenomenon in mammals is t-haplotype in mice. The mouse t-haplotype is a selfish variant region on chromosome 17, in nature transmitted as a unit. Male mice homozygous for t haplotype are sterile, but heterozygotes transmit the t haplotype up to 99% of their progeny. This is believed to be caused by motility differences between sperm carrying the t haplotype and wild-type sperm from the same heterozygous male. The concrete mechanism of the postulated sperm competition in favour of t haplotype carrying sperm was so far not fully illuminated. During this project, we worked with the hypothesis that the differences in sperm motility putatively responsible for transmission ratio distortion are triggered, at least in part, by metabolic causes. Our results from ATP and mitochondrial membrane potential (MMP) comparison indeed suggest that there are metabolic dissimilarities in sperm from the different genotypes of t (t/t, t/+, +/+). Specifically, our data show that there is significantly less ATP in t/t sperm when compared to the other two genotypes. Likewise, sperm from t/t mice also seem to have lower MMP, suggesting that...

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