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Noncovalent Interactions of Biologically Active Compounds
Osifová, Zuzana ; Dračínský, Martin (advisor) ; Lyčka, Antonín (referee) ; Sýkora, Jan (referee)
This thesis deals with the employment of nuclear magnetic resonance (NMR) spectroscopy in the studies of non-covalent interactions of biologically active compounds. The introduction serves as a brief overview of the literature, pointing out the milestones in determining and studying the most common non-covalent interactions - hydrogen bonding, π-π stacking and halogen bonding - by NMR spectroscopy. This part also includes the basics of this method to ensure that the results presented and the principles used will also be understandable for readers outside the field. Similarly, the employment and limitations of theoretical calculations are shortly discussed. The thesis aimed to determine the role of non-covalent interactions in biologically active compounds, including hydrogen bonding of modified nucleobases, interactions influencing ketoenol-diketo tautomerism of pharmacologically active curcuminoids, and the role of hydrogen bonding and amino acid sequence in the adoption of secondary structure in short peptides. Methylation and oxidation are nucleobases' most common natural modifications, causing changes in their hydrogen bonding pattern and preferences. We found that N-methylation of adenine residue stabilises its hydrogen-bonded base pairs with thymine and that these base pairs tend to adopt...
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Interactions of prosthetic material with proteins
Osifová, Zuzana ; Hudeček, Jiří (advisor) ; Martínek, Václav (referee)
This bachelor thesis deals with the problem of ocular prostheses. This problem is in thesis widely researched. Emphasis is placed on protein adsorption onto synthetic surface. Experimental part of the thesis focuses on lysozyme adsorption onto polymethylmethacrylate surface. Protein adsorption was lower than the specified limit of detection. According to experimental data was this amount lower than 5,37 μg/cm2 . In the thesis was conducted survey on secondary education students' awareness of ocular prosthesis. The results show slightly greater awareness of science-oriented students.
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Intermolecular Hydrogen Bonds Studied by NMR Spectroscopy
Osifová, Zuzana
N-methylation of nucleic acids is one of the most important epigenetic mechanisms in organisms and viruses and it is observed in connection with physiological or pathological processes in human body. For example, the N- methylation of adenine to give N6 -methyladenine is associated with development of obesity or Alzheimer disease. These systems can be successfully studied by low- temperature NMR spectroscopy because the bond between a purine ring and its substituent has a significant double-bond character and, due to restricted rotation around this bond, two sets of signals are observed in low-temperature NMR spectra. This diploma thesis is focused on research of N-methylated nucleobases' rotamer equilibria and the free-energy changes associated with the hydrogen-bonded base pair formation via simple and straightforward method based on NMR spectroscopy monitoring. We proved that the rotamer equilibria of N-methylated adenine derivatives are dependent on temperature, solvent and bonding partner concentration. We also obtained the geometry of formed intermolecular complexes of N-methylated adenine derivatives with thymine and the free-energy changes associated with their formations via newly developed method based on chemical shift changes dependent on bonding partner concentration. All obtained data...
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Intermolecular Hydrogen Bonds Studied by NMR Spectroscopy
Osifová, Zuzana ; Dračínský, Martin (advisor) ; Blahut, Jan (referee)
N-methylation of nucleic acids is one of the most important epigenetic mechanisms in organisms and viruses and it is observed in connection with physiological or pathological processes in human body. For example, the N- methylation of adenine to give N6 -methyladenine is associated with development of obesity or Alzheimer disease. These systems can be successfully studied by low- temperature NMR spectroscopy because the bond between a purine ring and its substituent has a significant double-bond character and, due to restricted rotation around this bond, two sets of signals are observed in low-temperature NMR spectra. This diploma thesis is focused on research of N-methylated nucleobases' rotamer equilibria and the free-energy changes associated with the hydrogen-bonded base pair formation via simple and straightforward method based on NMR spectroscopy monitoring. We proved that the rotamer equilibria of N-methylated adenine derivatives are dependent on temperature, solvent and bonding partner concentration. We also obtained the geometry of formed intermolecular complexes of N-methylated adenine derivatives with thymine and the free-energy changes associated with their formations via newly developed method based on chemical shift changes dependent on bonding partner concentration. All obtained data...
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Interactions of prosthetic material with proteins
Osifová, Zuzana ; Hudeček, Jiří (advisor) ; Martínek, Václav (referee)
This bachelor thesis deals with the problem of ocular prostheses. This problem is in thesis widely researched. Emphasis is placed on protein adsorption onto synthetic surface. Experimental part of the thesis focuses on lysozyme adsorption onto polymethylmethacrylate surface. Protein adsorption was lower than the specified limit of detection. According to experimental data was this amount lower than 5,37 μg/cm2 . In the thesis was conducted survey on secondary education students' awareness of ocular prosthesis. The results show slightly greater awareness of science-oriented students.
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