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Detection of selected fruit species in plant-based foodstuffs through instrumental analysis and methods of molecular biology
Ondruch, Petr ; Langová, Denisa (referee) ; Fialová, Lenka (advisor)
Food is often subject to adulteration, including fruit products containing strawberries. This work deals with the detection of strawberries in model and real fruit products, so that possible falsification can be detected. The aim of the work was the preparation of model and real fruit products, their analysis using selected instrumental and molecular-biological methods and mutual comparison of the results of these methods. Model mixtures of fruit products were prepared and commercial ones were purchased. The content of polyphenols and flavonoids was determined by UV-VIS spectrophotometry, which were characterized in more detail by HPLC. DNA was isolated from the fruit purees, suitable primers confirmed the amplifiability and formation of a specific product for strawberry, and this product was verified by electrophoretic control. In the instrumental part, certain substances were detected by extraction from model and commercial mixtures and the HPLC method, and the amount of selected substances was determined. The lowest amount of strawberry DNA that could be detected by selected primers in the PCR method was 0.7 ng /µl, in commercial mixtures this DNA could not be detected. In the HPLC method, the strawberry was detected in all samples by focusing on a particular fingerprint and the amount of pelargonidin-3-glucoside in the samples. The instrumental method can be considered more suitable in this case.
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Bioflavouring of beer using new Czech hop varieties
Ondruch, Petr ; Diviš, Pavel (referee) ; Pořízka, Jaromír (advisor)
This diploma thesis focuses on bioflavouring of beer using new czech hop varieties. Dry hopping is a phenomem, that contributes to the attractiveness and diversity of beer and is nowadays gaining in popularity. The aim of this work was to determine the effect of dry hopping on sensory profile and analytical parametrs of dry-hopped beers using czech hop varieties. Sensory evaluation and chemical analysis of these beers were taken. The chemical analysis included determination of the basic beer characteristics, total phenolic a flavonoid content, antioxidant activity and the concentration of selected organic acids and chemical elements. It was found out that dry-hopped beers compared to the reference beer presented higher bitterness, antioxidant activity and contained higher amount of flavonoids. Also an increase in pottasium, manganese, copper, calcium and malic acid levels was reported. The pH of beer samples was not affected by dry hopping. Sensory evaluation showed that dry hopping had a positive influence on the overall enjoyment of the beer, which was mainly determined by the higher aroma and more intense bitterness. By summaring all the data, it is obvious that dry hopping of beer using new czech hop varieties has a positive effect on the quality of the final beer and these hop varities are suitable for further use in the beer industry.
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Bioflavouring of beer using new Czech hop varieties
Ondruch, Petr ; Diviš, Pavel (referee) ; Pořízka, Jaromír (advisor)
This diploma thesis focuses on bioflavouring of beer using new czech hop varieties. Dry hopping is a phenomem, that contributes to the attractiveness and diversity of beer and is nowadays gaining in popularity. The aim of this work was to determine the effect of dry hopping on sensory profile and analytical parametrs of dry-hopped beers using czech hop varieties. Sensory evaluation and chemical analysis of these beers were taken. The chemical analysis included determination of the basic beer characteristics, total phenolic a flavonoid content, antioxidant activity and the concentration of selected organic acids and chemical elements. It was found out that dry-hopped beers compared to the reference beer presented higher bitterness, antioxidant activity and contained higher amount of flavonoids. Also an increase in pottasium, manganese, copper, calcium and malic acid levels was reported. The pH of beer samples was not affected by dry hopping. Sensory evaluation showed that dry hopping had a positive influence on the overall enjoyment of the beer, which was mainly determined by the higher aroma and more intense bitterness. By summaring all the data, it is obvious that dry hopping of beer using new czech hop varieties has a positive effect on the quality of the final beer and these hop varities are suitable for further use in the beer industry.
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Detection of selected fruit species in plant-based foodstuffs through instrumental analysis and methods of molecular biology
Ondruch, Petr ; Langová, Denisa (referee) ; Fialová, Lenka (advisor)
Food is often subject to adulteration, including fruit products containing strawberries. This work deals with the detection of strawberries in model and real fruit products, so that possible falsification can be detected. The aim of the work was the preparation of model and real fruit products, their analysis using selected instrumental and molecular-biological methods and mutual comparison of the results of these methods. Model mixtures of fruit products were prepared and commercial ones were purchased. The content of polyphenols and flavonoids was determined by UV-VIS spectrophotometry, which were characterized in more detail by HPLC. DNA was isolated from the fruit purees, suitable primers confirmed the amplifiability and formation of a specific product for strawberry, and this product was verified by electrophoretic control. In the instrumental part, certain substances were detected by extraction from model and commercial mixtures and the HPLC method, and the amount of selected substances was determined. The lowest amount of strawberry DNA that could be detected by selected primers in the PCR method was 0.7 ng /µl, in commercial mixtures this DNA could not be detected. In the HPLC method, the strawberry was detected in all samples by focusing on a particular fingerprint and the amount of pelargonidin-3-glucoside in the samples. The instrumental method can be considered more suitable in this case.
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