National Repository of Grey Literature 95 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
In vitro-cultures of medicinal plants - V
Sedlářová, Marcela ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
The elicitation of the therapeutically important cultures in vitro is a metod used for increasing the production of secondary metabolites. The effectiveness of elicitation depends on a complex interaction between the elicitor and the plant cell. The elicitation is influenced by many factors: the elicitors specificity, their concentration, the treatment interval, the growth phase of cell culture, the composotion of nutritional medium and the light. A wide array of external stimuli are capable of triggering changes in the plant cell which leads to a cascade of reactions, ultimately resulting in the formation and accumulation of secondary metabolites which helps the plant to overcome the stress factors. This work the influence of UV radiation on the flavonoids production in the callus cultures Genista tinctoria in vitro investigated. The highest production of flavonids was obtained at genistin under the exposure of callus culture to UV radiation of 254 nm wave lenght for the time 300 s with taking of sample after 48 hours. This callus culture grew on SH medium. The content of genistin was 3,03 %. Keywords: elicitation, elicitor, secondary metabolites, UV radiation, Genista tinctoria
Alkaloid isolation from Fontinalis antipyretica Hedw.
Malá, Marie ; Martin, Jan (advisor) ; Kašparová, Marie (referee)
Marie Malá, Isolation of alkaloids from Fontinalis antipyretica, thesis, Charles University in Prague, Faculty Of Pharmacy in Hradec Králové, 2012, 55 pages. The thesis Isolation of alkaloids from Fontinalis antipyretica, deals with quantification and isolation of substances of alkaloid character from above mentioned moss. The plant material collected in different localities in Czech Republic was extracted with methanol. The extract was subesquently used for isolation of alkaloids by HPLC method. The obtained amount of β-Carboline alkaloid was 2,4mg. The results of HPLC analysis (retention time, spectrum) of this sample were compared to harmalol standard and found the same. Finally the H NMR analysis proved, that the substance obtained by this experiment is not harmalol, but someother β-Carboline alkaloid.
The Influence on Production of Secondary Metabolits in Tissue Culture Hypericum perforatum (L.)
Machačková, Jana ; Martin, Jan (advisor) ; Tůmová, Lenka (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Farmacognosy Candidate Mgr. Jana Machačková Consultant PharmDr. Jan Martin, Ph.D. Title of Thesis The influence on production of secondary metabolits in tissue culture Hypericum perforatum (L.) Using of precursors is one of the biotechnological methods, where precursors are added to the culture medium. Callus and suspensions cultures of Hypericum perforatum L. were used. Fenylalanin and cinnamoyl acid in concentrations 20,0 g/l, 2,0 g/l and 0,2 g/l, resp. 25,0 g/l, 2,5 g/l and 0,25 g/l were used as precursors. The effect of precursors on the production of flavonoids was evaluated after 4, 24 and 168 hours. Cultures were cultivated on Murashige and Skoog medium with addition 2 mg/ml growthing hormon BAP. HPLC method for analysis was used. Generally, the highest increase of flavonoids was made by precursor fenylalanin in concentration B in callus and suspensions cultures. The top in production of quercitrin was detected in suspension cultures after application fenylalanin in concentration C after 168 hours cultivation (from 0,16 % to 0,22 %). Hyperosid was the most increased by fenylalanin in concentration C after 4 hours cultivation (from 0,24 % to 0,35 %). Cinnamoyl acid as precursor is not suitable for increase...
Interaction of selected anthocyanidins with farnesoid X receptor
Jeřábková, Jana ; Pávek, Petr (advisor) ; Martin, Jan (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Jana Jeřábková Supervisor: Doc. PharmDr. Petr Pávek, Ph.D. Title of diploma thesis: Interaction of selected anthocyanidins with farnesoid X receptor Human farnesoid X receptor (FXR) is a member of nuclear receptor superfamily that act as ligand-activated transcription factors. FXR binds to specific regulatory DNA regions and induces expression of many target genes. These regulated genes are involved in bile acid metabolism and transport, maintaining blood lipids, liporoteins and glucose homeostasis and also contribute to maintain intestinal bacterial balance, hepatoprotection and liver regeneration. The interest of recent studies is to test the range of FXR ligands for treatment and prevention of many diseases such as cholestais, cholesterol gallstone disease, steato-hepatitis, dyslipidemia, atherosclerosis, type 2 diabetes mellitus, metabolic syndrome, liver cancer and other forms of cancer such as breast cancer. In this experimental diploma thesis we are focused on testing of potencial ligands of human farnesoid X receptor from the group of natural plant pigments anthocyanidins (cyanidin, delphinidin, malvidin, pelargonidin, peonidin and petunidin) using the human hepatoma cell line...
Preparation of strategy for studying interaction of human constitutive androstane receptor with steroid receptor coactivator-1
Šachová, Ivana ; Martin, Jan (advisor) ; Mladěnka, Přemysl (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Ivana Šachová Supervisors: PharmDr. Jan Martin, PhD., Dipl. Ing. Mgr. Ferdinand Molnár, PhD. Title of diploma thesis: Preparation of strategy for studying interaction of human constitutive androstane receptor with steroid receptor coactivator-1 The aim of this study was to express wild type and mutated synthetic human SRC1 central domains in E. coli BL21 DE3 cells. Expressed recombinant proteins were purified afterwards and used for investigation of SRC1-CAR protein interaction using pull down experiments. The constitutive androstane receptor (CAR), a nuclear receptor (NR), is a transcriptional regulator, which influences the expression of various proteins, such as enzymes of biotransformation and transporters important for metabolism of both endogenous and exogenous compounds. CAR can be fully active only in presence of coactivators, such as steroid receptor coactivator-1 (SRC1). The most important fragment of SRC1 for binding to NRs is the central domain, made of three α-helical Leu-X-X-Leu-Leu (LXXLL) L1, L2, L3 motifs, in where L is a leucine and X represents any amino acid. hSRC1, as well as hCAR, can be successfully produced by expression of cDNA of interest in E. coli strains. In case of SRC1,...
Hypericum perforatum in vitro- abiotická elicitace
Majerová, Jitka ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Farmacognosy Candidate Mgr. Jitka Majerová Consultant Doc. PharmDr. Lenka Tůmová, CSc. Title of Thesis Hypericum perforatum in vitro- abiotická elicitace The effect of cerium chloride at 3 different levels of concentration (4,0571x 10-3 mol/l, 4,0571x 10-4 mol/l, 4,0571x 10-5mol/l) on production of hyperoside in cell and suspension culture of Hypericum perforatum L. was studied. The sample were taken after elicitation in fixed time period 0, 6, 12, 24, 48, 72 and 168 hours after elicitation. The colntrol sample (without elicitor treatment) ware taken after 24 and 168 hours. The sample ware analysed by HPLC method. Cerium chloride influenced hyperoside production in suspension and callus cultures. The highes content of hyperosid (0,27 mg/g DW) ware reached in suspension culture after cerium chloride treatment at concentration of c2 (4,0571x 10-4 mol/l) after 48 hours. Callus and suspension cultures did not release any hyperoside into nutrient medium.
The Effect of Elicitation on Production of Secondary Substances in Cultures in vitro
Špandelová, Veronika ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
The effect of elicitation on production of secondary substances in cultures in vitro Plant secondary metabolites are important sources for pharmaceutical, chemical and food industry. Elicitation is one of the possibilities how to increase the production of secondary subtances in cultures in vitro. The effect of abiotic elicitor 2- (3-brombenzylsulfanyl)pyridine-4-karbothioamide with antituberculotic activity on the isoflavonoids production Genista tinctoria callus and suspension culture was tested in this study. The elicitor was applied in three concentrations. Elicitation was run in this time intervals: 6, 12, 24, 48, 72 and 168 hours. Cultures were cultivated on nutrient medium, that described Shenk and Hildebrant with the addition of 0,5 mg/l of 2,4- dichlorfenoxyacetic acid and 0,1 mg/l of kinetin. The content of isoflavonoids was determined by HPLC. The highest content of isoflavonoids (0,03 %) in callus culture was found after the application of elicitor's solution in concentration of c3 = 2,96 x 10-5 mol/l after 24 hours of treatment. The maximum production of daidzein (0,02 %) in callus culture was detected after 48 hours of elicitor treatment in concentartion of c1 = 2,96 x 10-3 mol/l and after 24 hours of elicitor treatment in concentration of c3 = 2,96 x 10-5 mol/l. Suspension culture...
Possibilities of affecting of secondary metabolites production in culture Silybum marianum in vitro
Kunzová, Lucie ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
1 ABSTRAKT Mgr. Lucie Kunzová Possibilities of affecting of secondary metabolites production in culture Silybum marianum in vitro Elicitation is the method making use defensive mechanism of plants to increasing poduction of secondary metabolites in plants and cultures in vitro. The effect of 6, 12, 24, 48, 72 and 168 hours influence by three concentrations of the abiotic elicitor (3-jodo-4-methylfenyl)amide 5-methylpyrazine-2-karboxylic acid on the flavonolignans production in Silybum marianum callus and suspension culture was monitored in this study. The in vitro culture was cultivated on Murashige-Skoog medium with the addition of 10 mg/l of α-naphtylacetic acid as a growth regulator. The content of flavonolignans was determined by HPLC. The maximum content of flavonolignans (0,03 %) in callus culture was demostrated after 24 hours of elicitation (c1 = 2,83 · 10-3 mol/l). The maximum content of flavonolignans (0,04 %) in suspension culture was demonstrated after 48 hours of elicitation (c3 = 2,83 · 10-5 mol/l).
In vitro cultures of medicinal of plants - VIII
Špandelová, Veronika ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
In vitro cultures of medicinal of plants - VIII Method of elicitation is one of the possibilities how to increase production of secondary metabolites in cultures in vitro. The effect of abiotic elicitor (3-jodo-4-methylfenyl)amide 5- terc-butyl-6-chloropyrazine-2-karboxylic acid in three concentrations on the flavonolignans and flavonoid taxifolin production in Silybum marianum callus and suspension culture was tested in this study. Elicitation was run in this time intervals: 6, 12, 24, 48, 72 and 168 hours. Cultivation proceeded on Murashige - Skoog medium with the addition of 10 mg/l of α- naphtylacetic acid. The content of flavonolignans and flavonoid taxifolin was determined by HPLC. The maximum content of flavonolignans (0,05%) in callus culture was found after 168 hours of elicitor treatment in concentration of c2 = 2,33 x 10-4 mol/l. The maximum production of silychristin (0,04%) was found after 6 hours of affecting elicitor c1 = 2,33 x 10-3 mol/l. There wasn't statistical significant increasing of flavonolignans and taxifolin production in suspension culture. The maximum production of silybin A (0,01%) in medium of suspension culture was found after 24 hours of elicitor treatment in concentration of c1 = 2,33 x 10-3 mol/l.
Silybum marianum in vitro-abiotic elicitation.
Adamová, Eva ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
Elicitation is the method to increase the production of secondary metabolites in plant tissue. Elicitation by electric field is one of the method of abiotic elicitation to stimulate plants to produce higher amount of secondary metabolites. In vitro cultures of Silybum marianum were cultivated on Murashige-Skoog medium. Suspension cultures were exposed to electric field (50 mA, voltage 5, 7, 8, 10, 15, 20, 24 V) during 10, 30 and 60 minutes. The content of silymarin complex components was measured by (HPLC). The maximal content of taxifolin (0.04 %), silychristin (0.28 %), silydianin (0.06 %) in suspension culture was detected after elicitation by electric field 8V (10 minutes, 6 hours). The maximal content of taxifolin, silychristin, silydianin in suspension culture was detected after elicitation by electric field 8V (60 minutes, 6 hours) and 10V (60 minutes, 24 hours). The significant increase of silymarin complex components were not detectable after the other values of voltage .

National Repository of Grey Literature : 95 records found   1 - 10nextend  jump to record:
Interested in being notified about new results for this query?
Subscribe to the RSS feed.