National Repository of Grey Literature 41 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Study and Evaluation of Topically Applied Ceramides onto Disrupted Skin Barrier
Svobodová, Marie ; Kováčik, Andrej (advisor) ; Vraníková, Barbora (referee)
Department of Pharmaceutical Technology Author: M S b S , Ph.D. Title of diploma thesis: Study and Evaluation of Topically Applied Ceramides onto Disrupted Skin Barrier Skin is the largest organ of the human body and serves several key functions, such as protection against external influences and regulation of body temperature. Healthy skin is able to maintain the integrity and stability of the organism due to its complex structure. The skin is composed of several layers of cells, including the stratum corneum (SC), which forms the main protective barrier. Ceramides (CER) are one of the main types of barrier lipids found in the SC. These lipid molecules, along with other lipids, create a hydrophobic matrix that protects the skin from dehydration and other external influences. The lack of CER in the skin barrier leads to the disruption of its function and the worsening of the skin condition. This is associated with many diseases, such as dermatitis, psoriasis, and others. The study of CER in the SC is important to understand the mechanisms of skin protection and to develop new therapeutic approaches for the treatment of skin diseases. Topical administration of barrier lipids, especially CER, is one possible treatment route for skin diseases. Therefore, the aim of this work was to prepare...
2-hydroxyethyl cellulose gels with novel polyamidoamine dendrimers for the skin application of Imiquimod
Prokopová, Michaela ; Paraskevopoulos, Georgios (advisor) ; Kováčik, Andrej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Supervisor: Dr. Georgios Paraskevopoulos, Ph.D. Author: Michaela Prokopová Title of the thesis: 2-hydroxyethyl cellulose gels with novel polyamidoamine dendrimers for the skin application of Imiquimod Imiquimod (IMQ) is a medicinal substance used in the therapy of skin diseases (carcinomas). IMQ's hydrophobicity does not allow its incorporation in hydrophilic gels, which are the most used gel formulations for topical application. Dendritic molecules, which are highly branched polymers, could potentially increase the solubility of IMQ in hydrophilic formulations for the preparation of more concentrated hydrophilic dosage forms. The purpose of this work was to increase the concentration of IMQ in hydrophilic formulations using dendritic molecules. First, aqueous solutions containing IMQ were prepared, in the presence or absence of different generations and concentrations of dendritic molecules. The pH of the solutions was adjusted to 5,5, which corresponds to the pH of the skin. After that, 2-hydroxyethyl cellulose was used as gelling agent to prepare hydrophilic gels as preferable formulations for topical application. Selected solutions and gel formulations, were used for permeation experiments on human...
2-hydroxyethyl cellulose gels with novel polyamidoamine dendrimers for the skin application of Imiquimod
Prokopová, Michaela ; Paraskevopoulos, Georgios (advisor) ; Kováčik, Andrej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Supervisor: Dr. Georgios Paraskevopoulos, Ph.D. Author: Michaela Prokopová Title of the thesis: 2-hydroxyethyl cellulose gels with novel polyamidoamine dendrimers for the skin application of Imiquimod Imiquimod (IMQ) is a medicinal substance used in the therapy of skin diseases (carcinomas). IMQ's hydrophobicity does not allow its incorporation in hydrophilic gels, which are the most used gel formulations for topical application. Dendritic molecules, which are highly branched polymers, could potentially increase the solubility of IMQ in hydrophilic formulations for the preparation of more concentrated hydrophilic dosage forms. The purpose of this work was to increase the concentration of IMQ in hydrophilic formulations using dendritic molecules. First, aqueous solutions containing IMQ were prepared, in the presence or absence of different generations and concentrations of dendritic molecules. The pH of the solutions was adjusted to 5,5, which corresponds to the pH of the skin. After that, 2-hydroxyethyl cellulose was used as gelling agent to prepare hydrophilic gels as preferable formulations for topical application. Selected solutions and gel formulations, were used for permeation experiments on human...
Study and Evaluation of Topically Applied Ceramides onto Disrupted Skin Barrier
Svobodová, Marie ; Kováčik, Andrej (advisor) ; Vraníková, Barbora (referee)
Department of Pharmaceutical Technology Author: M S b S , Ph.D. Title of diploma thesis: Study and Evaluation of Topically Applied Ceramides onto Disrupted Skin Barrier Skin is the largest organ of the human body and serves several key functions, such as protection against external influences and regulation of body temperature. Healthy skin is able to maintain the integrity and stability of the organism due to its complex structure. The skin is composed of several layers of cells, including the stratum corneum (SC), which forms the main protective barrier. Ceramides (CER) are one of the main types of barrier lipids found in the SC. These lipid molecules, along with other lipids, create a hydrophobic matrix that protects the skin from dehydration and other external influences. The lack of CER in the skin barrier leads to the disruption of its function and the worsening of the skin condition. This is associated with many diseases, such as dermatitis, psoriasis, and others. The study of CER in the SC is important to understand the mechanisms of skin protection and to develop new therapeutic approaches for the treatment of skin diseases. Topical administration of barrier lipids, especially CER, is one possible treatment route for skin diseases. Therefore, the aim of this work was to prepare...
Spray drying of drug suspension in lactose solution
Vlková, Klára ; Svačinová, Petra (advisor) ; Kováčik, Andrej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical Technology Consultant: PharmDr. Petra Svačinová, Ph.D. Student: Klára Vlková Title of Thesis: Spray drying of a drug suspension in lactose solution Spray drying can be used to increase the solubility and thus bioavailability of drugs that are poorly soluble in water. In the present work, particles from a suspension of meloxicam in lactose solution were prepared by spray drying. The effect of the nozzle size of 0.7 mm and 1.4 mm, the effect of drug concentration of 0.5 g/100 ml and 1 g/100 ml, and the effect of lactose concentration on the properties of the resulting particles were investigated. The lactose solutions used had concentrations of 15, 20 and 25 %. The appearance and size of the formed particles were evaluated using optical and scanning electron microscopy (SEM). The amount of drug released was investigated using dissolution and the thermal characteristics of the particles were evaluated using differential scanning calorimetry (DSC). The evaluation by SEM and DSC was then performed again 6 months after the first measurement. The resulting particles were spherical in shape with no surface irregularities. Some of them contained clusters of meloxicam particles. At the second observation after 6 months,...
Study of Topical Ceramide Application
Kulhánková, Roksolana ; Kováčik, Andrej (advisor) ; Vraníková, Barbora (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Author: Roksolana Kulhánková Supervisor: doc. PharmDr. Andrej Kováčik, Ph.D. Title of diploma thesis: Study of topically administred ceramides The human stratum corneum (SC) is the uppermost layer of the epidermis, which is made up of several layers of corneocytes. The space between the corneocytes is filled with barrier lipids, i.e., ceramides, cholesterol, and fatty acids, which are represented in an equimolar ratio. The arrangement of intercellular lipids has a significant effect on the barrier properties of the skin. Damaged skin barrier (e.g., in atopic dermatitis) is associated with a lack of lipids in the SC lipid matrix, which is reflected in increased trans-epidermal water loss and deterioration of skin quality (e.g., severe dryness, flaking, and cracks). One of the potential ways to restore the skin barrier is the topical administration of barrier lipids, especially ceramides (Cer). The goal of this work was to study the effect of topically administered Cer (very long-chain Cer AP alone or in combination with ultra-long-chain Cer EOS) on model-damaged SC and their effect in the process of restoring barrier function. Several topically administered formulations (creams) were prepared, which...
Evaluation of viscoelastic parameters of co-processed materials and their components
Balušíková, Nikola ; Svačinová, Petra (advisor) ; Kováčik, Andrej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical Technology Consultant: PharmDr. Petra Svačinová, Ph.D. Student: Nikola Balušíková Title of Thesis: Evaluation of viscoelastic parameters of co-processed materials and their components. Co-processed excipients (CPE) are defined as the combination of two or more substances, which are physically modified by an appropriate manufacturing process (e.g. wet granulation, spray-drying). These types of excipients are currently increasingly used for production of tablets by direct compression technology. Due to the availability of a wide range of initial materials for their production, there is a possibility to obtain large number of combinations with required characteristics and better properties than the simple physical mixtures. Although the composition of CPE may be very similar, the small changes in the component's characteristics can make them behave differently during compression. The thesis is focused on the evaluation of the viscoelastic properties of selected CPE and their components (Cellactose 80, Avicel CE 15, Avicel DG, Avicel HFE 102, MicroceLac 100, Arbocel A 300, Arbocel M 80, Avicel PH1 02, Guar gum, Mannitol, Di-Cafos D 160, Lactochem) using the stress relaxation test at maximal compression force of 5 kN...
The human skin barrier model containing stripped stratum corneum
Soukupová, Romana ; Pullmannová, Petra (advisor) ; Kováčik, Andrej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Author: Romana Soukupová Supervisor: Mgr. Petra Pullmanová, Ph.D. Title of diploma thesis: The human skin barrier model containing stripped stratum corneum The uppermost layer of the epidermis, the stratum corneum, acts as a protective skin barrier. It contains a lipid matrix, which is made up of ceramides, cholesterol, and free fatty acids. The composition and arrangement of these lipids are necessary for the proper function of the skin barrier. Corneocytes, which do not have nuclei, are distributed in the lipid matrix. They are surrounded by insoluble proteins with covalently bound lipids on their surface, creating the corneocyte lipid envelope. The aim of this diploma thesis was to prepare models of the human skin barrier using an isolated stratum corneum with a preserved corneocyte lipid envelope and without a preserved corneocyte lipid envelope, and then monitor and compare the lipid arrangements in these models. Obtaining the stratum corneum from the skin samples was necessary to prepare the models. Human skin lipids were extracted from isolated stratum corneum and part of this delipidated stratum corneum was saponified. Skin lipids were purificated by column chromatography, and their...
Investigation of process parameters and their influence on spray dried particles properties
Grázová, Marcela ; Svačinová, Petra (advisor) ; Kováčik, Andrej (referee)
1. ABSTRACT Charles University, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical Technology Supervisor: PhamDr. Petra Svačinová, Ph.D. Consultant: Ing. Petr Koukal, Ph.D. Student: Marcela Grázová Title of Thesis: Investigation of process parameters and their influence on spray dried particles properties. The aim of the thesis was to determine to what extent it is possible to influence the particle size of the products by setting the parameters of the dryer. This particle size largely depends on the scale of the dryer used. Therefore, the aim was to determine whether a laboratory-scale spray dryer can either produce particles of variable size or particles of such size, that is typically obtained from production-scale devices. Particles prepared of meloxicam dispersions in hydroxypropylmethylcellulose phthalate were used for evaluation. A variety of input parameters for spray drying (drying gas inlet temperature, stock solution flow, concentration, and spray nozzle diameter) was used to prepare several batches of the product. The methodology Design of Experiments was used to identify the influence of individual input parameters on the product properties (particle size, glass transition temperature, and residual solvent content). Individual batches of the products were characterized by 1 H...
Study of Topical Ceramide Application
Kulhánková, Roksolana ; Kováčik, Andrej (advisor) ; Vraníková, Barbora (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Author: Roksolana Kulhánková Supervisor: doc. PharmDr. Andrej Kováčik, Ph.D. Title of diploma thesis: Study of topically administred ceramides The human stratum corneum (SC) is the uppermost layer of the epidermis, which is made up of several layers of corneocytes. The space between the corneocytes is filled with barrier lipids, i.e., ceramides, cholesterol, and fatty acids, which are represented in an equimolar ratio. The arrangement of intercellular lipids has a significant effect on the barrier properties of the skin. Damaged skin barrier (e.g., in atopic dermatitis) is associated with a lack of lipids in the SC lipid matrix, which is reflected in increased trans-epidermal water loss and deterioration of skin quality (e.g., severe dryness, flaking, and cracks). One of the potential ways to restore the skin barrier is the topical administration of barrier lipids, especially ceramides (Cer). The goal of this work was to study the effect of topically administered Cer (very long-chain Cer AP alone or in combination with ultra-long-chain Cer EOS) on model-damaged SC and their effect in the process of restoring barrier function. Several topically administered formulations (creams) were prepared, which...

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