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Dynamic saturation optical microscopy using photoswitchable proteins
Kolářová, Marie ; Benda, Aleš (advisor) ; Obšil, Tomáš (referee)
Fluorescence microscopy is an essential technique for live cell imaging. One of its drawbacks is a rather low diffraction limited spatial resolution, which is described by Abbe diffraction law. Therefore, in the last decade a lot of new methods improving spatial resolution were developed. One of them is dynamic saturation optical microscopy (DSOM) that is based on spatial monitoring of reversible transition kinetics between bright and dark states of fluorophores. The dark state is possible to obtain for example by using reversibly photoswitchable fluorescent proteins such as Dronpa and its variants. These proteins undergo reversible transition from fluorescent to nonfluorescent state after irradiation by blue and ultraviolet light. In my work I focus on employing the kinetics of controllable photoswitching of Dronpa in improving the overall image quality, including the spatial resolution. The experiments were performed on yeasts expressing selected proteins labelled with Dronpa. Firstly, photoswitching behaviour of Dronpa was confirmed. Secondly, experimental conditions were optimized by studying dependence of switching rate on laser intensities and on excitation wavelength and by studying protein photostability. Experiments were performed on different timescales and for various proteins. Using the optimal...
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The conditions of entering into marriage in comparison of the canonic law with the family law in the Czech Rebublic
Kotlář, Václav ; Kolářová, Marie (advisor) ; Hrdina, Antonín (referee)
Anglická anotace This thesis deals with the marriage law. Its aim is to study an approach to the institute of marriage from the point of view of the canon law in Roman Catholic Church in contrast to the family law in the Czeh Republic in focus on the conditions which are important for entering into marriage. In the first instance the thesis concerns with the specification of marriage in both branches of law, then the aims and effects of marriage, the form of entering into marriage, the suitable subjects for marriage including the subjects that have the right to marry somebody and also the issue of validity of marriage. Key word: marriage, validity, canon law, marriage law, conditions
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Freedom in the view of law theology and philosophy
Kolářová, Marie ; Hrdina, Antonín (referee) ; Plavec, Karel (referee)
Resume FREEDOM IN THE VIEW OF LAW THEOLOGY AND PHILOSOPHY The study is focused in the first part on the conception of freedom and divergence between freedom as general term and freedom of choice. Some authors reflect freedom, but they do not specify your conception of freedom. Man's freedom according to Gaudium et spes (17) means: "Authentic freedom is an exceptional sign of the divine image within man." There is the crucial problem: "authentic freedom" - what it means. The study compares conception of freedom by these outstanding theologians: Anselm of Canterbury, S. Augustin, Bernard of Clairvaux, Origenes and Karl Rahner. Accuracy of expressing is necessary for right understanding each other. Transcendental freedom according K. Rahner implies man's freedom as the foundation of human being. Everybody has transcendental freedom, man's dignity is not possible without freedom, otherwise would be man only an instrument destined to certain existence. Freedom of choice arises from transcendental freedom, but it does not imply man's arbitrariness, because everybody has own dignity and freedom, which belongs one person is the limit for each other person. Transcendental freedom is equal by each person contrary to freedom of choice, which is impacted of man's personality and also external circumstances. The study...
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The Altruistic Phenomenom of Living Kidney Donation
Bobek, Vít ; Tollarová, Blanka (advisor) ; Kolářová, Marie (referee)
The Altruistic Phenomenon of Living Kidney Donation This thesis considers experiences related to the altruistic phenomenon of living kidney donation. The aim is to describe and explain the motivations, attitudes and actions of those who have decided to donate one of their kidneys to a person with end-stage renal disease. The thesis is divided into two main sections: a theoretical part and research work carried out by the author. The theoretical part provides a short history of living kidney donation and explains the clinical basis for living donor kidney transplants. In addition, this section briefly introduces the transplant centre of the Institute for Clinical and Experimental Medicine, including its strategy and interests. The theoretical part also outlines the risks to the donor of unilateral removal of a kidney, the ethical issues of living kidney donation, psycho-social aspects, and the legal conditions of procuring a kidney from a living donor. The research component states the basic research question, giving rise to specific questions and interview questions. It introduces the research method chosen - the phenomenological approach - and describes the sample of respondents and their selection. An outline of the process of collecting and analysing the research data is also included in this...
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The Altruistic Phenomenom of Living Kidney Donation
Bobek, Vít ; Tollarová, Blanka (advisor) ; Kolářová, Marie (referee)
The Altruistic Phenomenon of Living Kidney Donation This thesis considers experiences related to the altruistic phenomenon of living kidney donation. The aim is to describe and explain the motivations, attitudes and actions of those who have decided to donate one of their kidneys to a person with end-stage renal disease. The thesis is divided into two main sections: a theoretical part and research work carried out by the author. The theoretical part provides a short history of living kidney donation and explains the clinical basis for living donor kidney transplants. In addition, this section briefly introduces the transplant centre of the Institute for Clinical and Experimental Medicine, including its strategy and interests. The theoretical part also outlines the risks to the donor of unilateral removal of a kidney, the ethical issues of living kidney donation, psycho-social aspects, and the legal conditions of procuring a kidney from a living donor. The research component states the basic research question, giving rise to specific questions and interview questions. It introduces the research method chosen - the phenomenological approach - and describes the sample of respondents and their selection. An outline of the process of collecting and analysing the research data is also included in this...
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Dynamic saturation optical microscopy using photoswitchable proteins
Kolářová, Marie ; Benda, Aleš (advisor) ; Obšil, Tomáš (referee)
Fluorescence microscopy is an essential technique for live cell imaging. One of its drawbacks is a rather low diffraction limited spatial resolution, which is described by Abbe diffraction law. Therefore, in the last decade a lot of new methods improving spatial resolution were developed. One of them is dynamic saturation optical microscopy (DSOM) that is based on spatial monitoring of reversible transition kinetics between bright and dark states of fluorophores. The dark state is possible to obtain for example by using reversibly photoswitchable fluorescent proteins such as Dronpa and its variants. These proteins undergo reversible transition from fluorescent to nonfluorescent state after irradiation by blue and ultraviolet light. In my work I focus on employing the kinetics of controllable photoswitching of Dronpa in improving the overall image quality, including the spatial resolution. The experiments were performed on yeasts expressing selected proteins labelled with Dronpa. Firstly, photoswitching behaviour of Dronpa was confirmed. Secondly, experimental conditions were optimized by studying dependence of switching rate on laser intensities and on excitation wavelength and by studying protein photostability. Experiments were performed on different timescales and for various proteins. Using the optimal...
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Christianity freedom - concept and problems of acknowledgement in canon law
Kolářová, Marie ; Hrdina, Antonín (advisor) ; Kašný, Jiří (referee) ; Šprunk, Karel (referee)
Christianity freedom - concept and problems of acknowledgement in canon law Key words: Theory and Philosophy of Law, Concept of Law, Freedom of a Christian, Morality, Rights of Catholics First part: The concept of freedom that is within philosophy and law theory understood in different meanings - distinction among transcendental freedom, freedom of choice and freedom in Christ. Second part: The concept of law; the relationship between law and values. The nature of canon law; is canon law a juridical or a theological science? School of thought in the contemporary debate about the nature of canon law. Law is one aspect of a complex social reality. I Third part: The nature of the Church; the visible and the invisible church. The renewed theological vision of the nature of the Church. The place of law in the Church. Two distinct ecclesiologies: one of the Church as sacrament and communion, the other of a juridicalIy organized perfect society. Fourth part: The close relationship of law norms and moral norms in the juristic regulation of the Church. The distinction between to conduct according moral and law norms. Framework of morality. Moral norms encourages persons to be virtuous. Fifth part: The fundamental human rights are .inseparably interconnected in the very person. The fundamental human rights...
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Freedom in the view of law theology and philosophy
Kolářová, Marie ; Hrdina, Antonín (referee) ; Plavec, Karel (referee)
Resume FREEDOM IN THE VIEW OF LAW THEOLOGY AND PHILOSOPHY The study is focused in the first part on the conception of freedom and divergence between freedom as general term and freedom of choice. Some authors reflect freedom, but they do not specify your conception of freedom. Man's freedom according to Gaudium et spes (17) means: "Authentic freedom is an exceptional sign of the divine image within man." There is the crucial problem: "authentic freedom" - what it means. The study compares conception of freedom by these outstanding theologians: Anselm of Canterbury, S. Augustin, Bernard of Clairvaux, Origenes and Karl Rahner. Accuracy of expressing is necessary for right understanding each other. Transcendental freedom according K. Rahner implies man's freedom as the foundation of human being. Everybody has transcendental freedom, man's dignity is not possible without freedom, otherwise would be man only an instrument destined to certain existence. Freedom of choice arises from transcendental freedom, but it does not imply man's arbitrariness, because everybody has own dignity and freedom, which belongs one person is the limit for each other person. Transcendental freedom is equal by each person contrary to freedom of choice, which is impacted of man's personality and also external circumstances. The study...
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The Process of Education of Patients in the Clinic of Nephrology IKEM after the Implementation of Quality Management System ISO 9001:2000
Kolářová, Marie ; Vrzáček, Petr (advisor) ; Pokorná, Eva (referee)
Final thesis is divided into two parts- theoretical and practical ones. It deals with the transplantology, first of all, with a process of education of patients after kidney transplantations. The theoretical part focuses on introduction a clinic and a general introduction about problems transplantations. The first chapters describe a historical summary of development of this discipline and as well as its contemporary position. Next it focuses on juridical and ethical aspects of transplantations. Finally, the theoretical part devotes the education in general. The entire theoretical part is dedicated to the clarification of complexity of this discipline and to the importance of reaching the best cooperation between patients, physicians and nursing staff by way of educational activity both before and after transplantation. The practical part describes a process of patients'preparations for transplantation and therapeutic possibilities after a kidney failure. Next chapters deal with a preparation and a creation of an educational process in the clinic of nephrology in the Institute of the Experimental and Clinical Medicine and its implementation to the Quality Management System ISO 9001:2000. The conclusion of the practical part presents opportunities how to improve the process of education. Through a...
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