National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Targeting of Viral Nanoparticles to CD44 via Hyaluronic Acid
Hustedová, Anna ; Španielová, Hana (advisor) ; Hubálek Kalbáčová, Marie (referee)
Hyaluronic acid (HA) is widely studied as a targeting moiety to CD44 overexpressing cancer cells. Various types of nanoparticles (NPs) were modified by HA. Virus-like particles (VLPs) derived from mouse polyomavirus are an interesting class of NPs that can be modified by various targeting agents to increase their potential as gene or drug delivery vehicles for e.g. theragnostic purposes. HA has not been tested as a targeting moiety on VLPs, hence this was the focus of the current study. HA (~14 kDa) was attached to the VLPs via a bispecific Bodipy-derived fluorescent probe. To test the targeting potential of HA on comparable non-viral NPs, nanodiamonds were prepared in a similar manner. NPs functionalized with HA, together with Bodipy-labeled control variants, were tested on interactions with MDA-MB-231 cells overexpressing CD44. The NP-cell interaction via CD44 was assessed by a competitive cell-binding assay, where non-labeled HA competed for HA-binding sites at CD44 with the NPs. CD44 specific cell interactions were detected in studies with HA functionalized nanodiamonds, whereas VLP-HA* associated with cells in a less specific manner. Control VLPs with polyethylene glycol (PEG) did not interact with the cells. Results indicate that the HA targeting strategy for the VLPs requires optimization to...
Diagnostics of Tick-borne Encephalitis: Present State and Future
Hustedová, Anna ; Španielová, Hana (advisor) ; Saláková, Martina (referee)
Tick-borne encephalitis is one of the most severe tick-borne neuroinfections in Europe and Asia. This thesis is focused on its source, Tick-borne encephalitis virus (TBEV). Diagnostics of TBEV is usually based on detection of specific anti-TBEV antibodies in patient's serum by using enzyme-linked immunosorbent assay. However antibodies that develop during the infection often cross-react with other flavivirus specific antibodies. As an antigen for ELISA tests the formalin inactivated virus is usually used, its production is restricted to laboratories with biosafety level 3. This thesis is seeking for possibilities of production of specific antigen for serological diagnostic of TBEV using heterologous expression systems. As the new antigens, parts of glycoprotein E and subviral particles were used. Production of antigen in the formo of subviral particles seems to be a good alternative to inactivated virus.

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