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Interaction of myricetin with human gut microflora
Hucková, Pavlína ; Hodek, Petr (advisor) ; Killer, Jiří (referee)
The intestinal microbiome contributes in immune system function. It contains a large number of microorganisms that interact with each other and thus affect the host. Currently, attention is being directed towards investigating the influence of the intestinal microbiome on hosts, but also on foreign substances. Foreign substances may influence its composition and subsequent microbial metabolism. Crohn's disease patients have been found to have lower bacterial representation of beneficial bacteria. Therefore it is appropriate to examine the intestinal microbiome of these patients and so understand in greater detail the influence of bacteria on the course of the disease progression and on the medication used. The RP-HPLC method were analysed the faecal samples collected (B, C, D), which were incubated at 0, 3 and 6 hours. The incubation took place the addition of myricetin in the McDougall buffer and BHI medium. The analysis was found that myricetin degradation takes place in faecal samples during incubation, regardless of the medium used. In the faecal sample B, degradation of myricetin occurs faster in the BHI medium than in the McDougall's buffer. In faecal samples C and D, degradation is similar in both media. From these results, it is impossible to judge which medium is more suitable for...
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Role of gut microflora in metabolism of drugs and carcinogens
Hucková, Pavlína ; Hodek, Petr (advisor) ; Kubíčková, Božena (referee)
We can consider intestinal microflora as another large organ of human body, that isn't sufficiently explored yet. It is very complex system with many functions for host organism. It contains a large number of bacteria, viruses and archea. Intestinal microflora has an important function on food decomposition and metabolism of foreign substances. Foreign substances has a positive or negative effect on intestinal bacteria. That's why the research focuses on metabolism of these substances and their effect on digestive tract disease. Research also focus at intestinal bacteria diversity which may be important in treating patients. Fecal samples (A, B, C) was incubated at time 0, 3 and 6 hours in two mediums (McDougall buffer and BHI medium). Incubated samples was analyzed by RP-HPLC method. Focus was on degradation of myricetin and his potential transformation into dihydromyricetin. Research proved, that degradation myricetin in 6 hours was at every fecal sample (A, B, C). It can not be evaluated what medium is best for the bacteria because in first fecal sample (A) there was a faster degradation myricetin in McDougallov's buffer then in BHI medium. Another sample (B) on the others side reacted faster in BHI medium then in McDougallov's buffer. The last sample (C) occured practically same degradation for...
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