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The expression and function of nucleophosmin (B23) during bovine preimplantation development
Benešová, Veronika ; Kaňka, Jiří (advisor) ; Krylov, Vladimír (referee)
Identification of genes that start their expression in different time of embryonal development is crucial for proper understanding of early mammalian embryogenesis. Genes that are differently expressed during this period are supposed to be important for normal preimplantation development. One of these genes is nucleophosmin, the multifunctional protein, which functions among others as a transporting protein between cytoplasm and nucleoli. It is involved in duplication of centrosomes, formation of ribosomes and functions as a chaperoning protein. Nucleophosmin shuttles rapidly between the nucleolus and cytoplasm. Its localization is cell cycle dependent and reflects the development of the nucleoli. For studying the effect of nucleophosmin on the bovine preimplantation development we used RNA interference that causes degradation of the target mRNA after injection of nucleophosmin double-stranded RNA. The average decrease of nucleophosmin mRNA abundance is 86.3 % between nucleophosmin dsRNA injected embryos and control group. We detected no differences in protein expression and localization of nucleophosmin between the groups by immunofluorescence method. Embryos in all groups were able to develop until the blastocyst stage, although there were significant difference between uninjected control group and...
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název v anglickém jazyce není uveden
Křenová, Martina ; Pelclová, Daniela (advisor) ; Černá, Milena (referee) ; Benešová, Veronika (referee)
The aim of our study was to evaluate the severity of hepatic and kidney damage retrospectively in the years between 2000 and 2004 with a focus on their reversibility, and to analyse the prognostic factors following Amanita phalloides (Am. ph., mainly hepatotoxic agent) and ethylene glycol (EG, nephrotoxic liquid) based on the calls made to the Czech Toxicological Information Centre and analyses made by toxicological laboratories. Analysis relied on data from medical records. A variety of clinical and laboratory parameters were collected. Fisher's test, Student's t-test and the calculation of linear correlation coefficients were used for statistical analysis. Twenty-four of 206 case-patients with EG intoxication were children. Fifty of 158 adult patients who survived, developed signs of nephrotoxicity. In 19 patients renal function normalized up to one month following the intoxication. Total 21 patients were followed-up as out-patients. Renal function completely recovered in 13 patients during 6 months and in three patients during 8-24 moths following the intoxication. The serum creatinine level was only mild elevated (119 |imol/l) in other two patients. However, their follow-up finished. In three patients renal damage persisted, serum creatinine level 138-200 (imol/1, in 19-26 months after discharge from...
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Physical activity as a preventive component of the lifestyle of seniors
Vlastníková, Věra ; Fiala, Zdeněk (advisor) ; Hlúbik, Pavol (referee) ; Benešová, Veronika (referee)
This dissertation deals with physical activity of senior women and its importance in health prevention. Current knowledge in the areas of gerontology, exercise physiology and kinanthropology was summarized in the theoretical part. For experimental part of the work, questionnaires were compiled and group and individual exercise programs were created and applied on a set of senior women. The objective of the dissertation was to assess the influence of these programs on civilization diseases risk factors and on some indicators of quality of life. Our study population was formed by healthy senior women recruited from the programs of senior university education in Hradec Králové. The study population was further divided into an experimental group which completed a group exercise program (G1; 18 subjects, 63±3 yrs), an experimental group which completed an individual exercise program according to our instructions (G2; 14 subjects, 65±5 yrs) and a control group without an exercise program (G3; 19 subjects, 69±4 yrs). Exercise programs were applied for 10 months, 2 hours/week and contained remedial exercises, strengthening activities, balance and relaxation exercises and walking. The variables examined included body weight (BW), body mass index (BMI), blood pressure (BP), resting heart rate, total...
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Quality of life and Lifestle in Czech children in town and village
Hlaváčková, Eva ; Hodačová, Lenka (advisor) ; Benešová, Veronika (referee) ; Hrubá, Drahoslava (referee)
Quality of life is a multidimensional concept that has been researched in many scientific fields. It has gained an important position especially in medicine, sociology, and psychology. The aim of the study was to monitor specific indicators of quality of life (QoL) and lifestyle in a representative sample of Czech children within an international study of the World Health organization (WHO), The Health Behaviour in School Aged Children: A WHO Cross National Study (HBSC), to analyze QoL in Czech children according to age, gender, and place of residence, and to evaluate it as it relates to other important factors associated with lifestyle (formal structure of the family, the amount of time spent together in the family, adaptation to school, subjectively perceived degree of demands for achievement in the school, social relationships, alcohol and tobacco consumption). The data were obtained using a questionnaire in 86 randomly selected elementary schools and multiannual secondary schools. The study methods emanated from the WHO recommendations. The target population for the research study was a representative sample of Czech children aged 11, 13, and 15, a total of 4,782 children. We monitored subjectively perceived QoL based on a subjective evaluation of the health status, the feeling of happiness, and...
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Pharmacovigilance in the Toxicological Information Centre
Urban, Michal ; Pelclová, Daniela (advisor) ; Benešová, Veronika (referee) ; Zacharov, Sergej (referee)
1 ABSTRACT Background The annual drug overdose rates have been increasing exponentially since the 90's worldwide. Toxicological information centre (TIC) represents a valuable source of information for evaluating the trends in the drug poisonings in Czech Republic. Aim of the study The purpose was to analyze the number and trends in the calls concerning poisonings due to central nervous system (CNS) affecting drugs, identify the reasons of medication errors caused by laymen, frequency and consequences of these errors across all age groups and also to analyze the numbers, causes, symptoms and severity of the paracetamol intoxications. Methods During the reference period the data from the enquiries were extracted from the TIC electronic database, discharge reports from the hospital were studied or phone call follow-ups with the patients were carried out to be able to evaluate the outcome of the poisonings. Results In the years 1997-2002 the number of calls caused by poisoning with tricyclic antidepressants and barbiturates decreased (by 366.7 % and 340,0 %, respectively) whereas the calls due to selective serotonin reuptake inhibitors and benzodiazepines overdose increased (by 1347.4 % and 359.8 %). The 0-5 years old children are at the highest risk of experiencing medication errors or accidental poisonings...
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Vliv přípravků nahrazujících použití sudů typu barrique na parametry bílých vín
Benešová, Veronika
The literary part of this thesis deals with the production of barrique and their alternatives. The thesis is mainly focused on chips. Additionally there is describes the factores which affecting the chips in wine. More specifically, what are chips effect on the fermentation of white wine. Finaly comparing barrique barrels with chips. The experimental part was based on the experiment, when chips were added to white wine varieties Malverina. Differences beteween variants were in moment of adding (must, wine) and the degreee of tasting (medium, heavy). For evaluate the sensory analysis experiment was attended by an expert team. The tasters rated the sample to a 100-point scoreboard and also to additional table, where they rated specific properities. In the case of variant, when the chips are added to wine, was additionally evaluated certain aromas. Determination of antiradical aktivity was measured by spectrophotometric measurements. Measured values showed that in the case of addition of chips to the must antiradical aktivity was lower than the control sample, probably because of alcohol, whose presence caused leaching of higher amounts of phenolic compounds, antioxidants. The highest antiradical activity showed sample with medium toasted chips.
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Avant-garde Foreign Feedbacks in the Review Avant-garde
BENEŠOVÁ, Veronika
The magazine Avantgarda was one of the periodicals, which has participated in completing the character of czechoslovakian culture in the course of twenties till thirtieth in 20. centrury. My theses deals with researching and analysing this magazine from 1925 to 1930, including the view of the historical events, it means the cultural factors in area off art, theather, literature in the proces. The primary goal is to analyse the different appearing years of this periodical, which has resised up, to finde out the following growth and heading at all, and to a certain extent to express the editorail´s orientation as well. One part of these theses builts the sond to the literatureproduction of the magazine, which are in the last phase primarily oriented at the foreign (in german written) contribution, attempt interpretation of them related to home avant-garde output.
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