National Repository of Grey Literature 126 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Study of the effect of zinc oxide nanoparticles on cell migration
Kutějová, Monika ; Zumberg, Inna (referee) ; Chmelíková, Larisa (advisor)
Zinc oxide nanoparticles (ZnO NPs) are widely used across various industries due to their properties, and with the development of nanomaterials, they are also finding applications in medicine. This bachelor’s thesis investigates the cytotoxic effects of these nanoparticles on cultured cell lines. The study focused on the human osteosarcoma cell line Saos-2 and the fibroblast cell line NIH-3T3. During in vitro experiments, the cells were exposed to different concentrations of ZnO NPs. The cytotoxicity of each concentration was assessed using the MTT assay, which provided information on cell viability. Based on the MTT assay results, appropriate concentrations of ZnO NPs were selected for conducting a Scratch assay, with the highest concentration corresponding to the determined inhibitory concentration for NIH-3T3. The cytotoxic effects were thus evaluated by studying cell migration. Subsequent statistical analysis determined the extent of cytotoxic effects of ZnO NPs depending on the concentration and cell type.
Study and optimization of mixotrophic growth of selected strains of autotrophic microorganisms
Miheeva, Alexandra ; Sikorová, Pavlína (referee) ; Sniegoňová, Pavlína (advisor)
This bachelor thesis investigated the production of microalgal metabolites in response to stress conditions. In recent decades, there has been a growing interest in the research and application of algae in various fields such as food, cosmetic, pharmaceutical, biotechnology and energy industries. The microalgae strains selected for this work are Desmodesmus quadricaudea (CCALA 464), Chlorella minutissima (CCALA 916), Desmodesmus subscapitus (CCALA 688), Scendesmus pleiomorphus (CCALA 460). The theoretical part of the work focuses on the description of selected genera of microorganisms, conditions of their cultivation and metabolites produced. Furthermore, stress factors and analytical methods used to identify and quantify the produced substances are discussed. In the practical part of the thesis, the cultivation procedure, biomass processing and metabolite analysis are described, together with a presentation of the results obtained. Algal strains Desmodesmus subscapitus, Scenedesmus pleiomorpfus and Chlorella minutissima showed the highest alcohol consumption in HPLC-RI analysis, making them the most suitable candidates for possible mixotrophic cultivation. Growth optimization of Chlorella minutissima was also performed and analyzed in this work. Its results are presented in the practical section.
Economical efficiency of selected crops growing in conventional and organic farming system - case study
The aim of the thesis is to compare the economic efficiency of selected crops culti-vated in organic and conventional agriculture. The analysis of economic efficiency covers the period from 2019 to 2023 and focuses on winter wheat and spring oats. The thesis consists of two main parts, with one part being a literature review. Here, economic efficiency is characterized, along with the organic and conventional ag-ricultural systems. A brief description of the history of agriculture in the Czech Republic, various crop cultivation methods, and a concise list of agricultural sub-sidies are provided. The practical part deals with crop yields, individual operations in winter wheat and spring oats cultivation. Purchase prices of cultivated crops are displayed, followed by the calculation of the economic efficiency of both cultivation methods.
Detection of pathogens in joint infections, including total endoprostheses using the BioFire method (Biomérieux)
This bachelor thesis deals with the detection of pathogens in joint infections including total joint replacements by the BioFire method (Biomérieux, France). Compared to conventional methods, the Biofire Joint Infection Panel (BioMérieux, France) offers simultaneous detection of up to 39 pathogens and antimicrobial resistance markers in about one hour of time.This method is a multiplex PCR system for rapid and comprehensive testing. It is an innovative method that, thanks to its speed and comprehensiveness, will allow clinical microbiologists and physicians to make timely decisions on appropriate antibiotic therapy. Early de-escalation of this antibiotic therapy will also be enabled through the timely and targeted deployment of the appropriate ATB. The theoretical part of the thesis discusses the major infections of joints and joint replacements, introduces the reader to the most important causative agents of these infections, and a section is also devoted to microbiological diagnosis. The practical part describes the classical multiplex PCR, the FilmArray multiplex PCR system and with it the BioFire Joint Infection Panel, which has been used in the analysis of synovial fluid in patients with suspected septic arthritis or periprosthetic infection. Data from the analysis are also evaluated in the practical part. The data are from March 2021 to June 2022 and are presented in the practical part in the form of tables and graphs. The conclusion of the paper summarizes the relevance of the method for routine diagnosis and answers the stated research questions.
Pseudomonas aeruginosa jako původce močových infekcí
ŽÁČEK, Jakub
This bachelor thesis deals with the microbiological laboratory diagnosis of urinary bacterial infections, with an emphasis on the gram-negative bacterium Pseudomonas aeruginosa. This bacterium is known for its high resistance and is often found as a causative agent of urinary infections in immunocompromised patients. The theoretical part of this bachelor thesis describes the structure of the urinary system, bacterial urinary infections as a whole, and the bacterium Pseudomonas aeruginosa itself. The methodological part, conducted in the microbiology section of the SYNLAB České Budějovice laboratory, subsequently describes the procedures for processing urine samples for bacteriological examination, including sampling, transport, and sample reception, pre-cultivation screening, and cultivation methods. Furthermore, methods of identification based on the biochemical properties of bacteria and identification using MALDI-TOF are described. Also included in the methodological part is the determination of bacterial sensitivity to antimicrobial agents using the disc diffusion method. The results obtained from the laboratory information system for the periods of November and December 2023 and January 2024 are evaluated and compared with the results of other authors. This work serves to verify the methods used and emphasizes the use of modern methods in the diagnosis of bacterial urinary infections.
Vliv CTF na utužení půdy a ekonomiku zemědělského podniku
The bachelor's thesis deals with the comparison of three methods of cultivation of Julia winter wheat. The work is focused on the cultivation method in the CTF system. The thesis explains what farming in the CTF system means and what benefits they bring to farmers. GPS systems that belong to automation in agriculture are also described. Attention is paid mainly to the cost of fuel consumption of used rigs and to excess crossings in the field.
Grape vine
Lubovská, Karolína ; Písaříková, Jana (referee) ; Korbička, Pavel (advisor)
The work Grape Vine explores the theme of the South Moravian wine landscape and the elements that are associated with it and most characteristic of it. The work highlights the practices by which this landscape is altered and cultivated for human use, in this case for the cultivation of grapes and the wine production. It focuses on the approach to the landscape and the specific ways in which different parts of it are approached through cultivation, selection and growth control in order to achieve the highest yields using the practices determined by the tradition of agriculture in this specific area.
Infection of salmonelosis, their source and diagnosis.
Valkus, Martin ; Veselá, Mária (referee) ; Vránová, Dana (advisor)
This bachelor‘s thesis deals with infection of salmonelosis, it’s source, prevention and diagnosis. Theoretical part includes general information about Salmonella species and its morphology. It describes pathogenesis, source of infection, clinical symptoms, treatment and diet recommendations. It analyzes statistics of number of infection occurrences in Czech Republic. It also deals with prevention of infection origin on schools, population foreknowledge and climatic effects. Experimental part of this thesis contains description of optimization procedure of identification Enterobacteriaceae by adjusting of pH of prepared medium MIU.
Study of 3D bacterial cellulose production using banana peels as a carbon source
Netopilík, Tibor ; Pernicová, Iva (referee) ; Kovalčík, Adriána (advisor)
This bachelor thesis deals with the study of 3D bacterial cellulose production using Komagataeibacter xylinus using banana peels as a carbon source. The theoretical part deals with the comparison of the properties of bacterial cellulose and plant cellulose, different methods of biotechnological production of bacterial cellulose and its use. The aim of the bachelor thesis was to find out whether it is possible to use banana peel as a carbon source for biotechnological production of bacterial cellulose. Banana peels are waste lignocellulosic material produced, for example, in the production of snacks or fruit or dairy beverages in the food industry. HPLC analysis showed that 1 l of hydrolyzate after enzymatic hydrolysis of 100 g of dry banana peels per 1 l of water contained 8.86 g of glucose and 10.46 g of fructose. The hydrolyzate was used as a carbon source for static and dynamic cultivation of Komagataeibacter xylinus. The yields of bacterial cellulose produced by using banana peels or glucose were compared. The use of banana peels hydrolyzate increased the yields of bacterial cellulose about 170 % in the case of static cultivation and about 220 % in the case of dynamic cultivation. Scanning electron micrographs of bacterial cellulose confirmed the morphological differences between bacterial cellulose produced statically and dynamically. Moreover, the morphology of bacterial cellulose was influenced by the kind of used carbon source.
Optimization of cultivation of carotenogenic yeasts on mixed waste substrates
Holub, Jiří ; Mikulíková, Renata (referee) ; Márová, Ivana (advisor)
The master thesis addresses the issue of cultivation of selected strains of carotenogenic yeasts on waste materials of the food industry using a laboratory bioreactor. Carotenogenic yeasts are able to produce highly valuable metabolites during cultivation, which are located predominantly in the lipid part of the cells. Particularly, they are carotenoids, ergosterol, coenzyme Q and fatty acids. The thesis is divided into two main parts, the theoretical part and the practical part. The theoretical part describes individual yeast strains, types of waste materials, produced metabolites and methods of their analysis. The experimental part deals with the processing of waste materials of the food industry, specifically animal fat, whey and spent coffee grounds into the form of substrates usable as nutrition sources for yeast cultivation. Furthermore, cultivations focused on the recovery of the monitored metabolites and their analysis by using HPLC/PDA and GC/FID assemblies were studied as well. The yeast strains Rhodotorula mucilaginosa (CCY 19-4-6), Rhodotorula kratochvilae (CCY 20-2-26), Rhodosporidium toruloides (CCY 062-002-001), Sporidiobolus pararoseus (CCY 19-9-6) a Cystofilobasidium macerans (CCY 10-1-2) were used in this work. As one of the best producing strains Sporidiobolus pararoseus (CCY 19-9-6) was found, which achieved very high productions of carotenoids, coenzyme Q and ergosterol.

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