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Exprese transgenu bphC u ječmene
Sedlářová, Kateřina
Polychlorinated biphenyls are toxic organic compounds, characterized by a low degradability in the environment and accumulation in the food chain. This is the reason, why decontamination of soils polluted with such substances is very important issue for today's society. Decontamination of areas infected by PCBs is very complicated due to its physical and chemical characteristics. Appropriate decontamination method is phytoremediation, which is used for removing polychlorinated biphenyls by natural processes of plants. Using the transformation of plants can increase the efficiency of phytoremediation. The thesis has been investigated in expression of remediation gene bphC/His at T2 generation of spring barley (Hordeum vulgare L.), variety Golden Promise at the DNA, RNA and protein levels. Expression of the transgene was detected using PCR, real-time PCR and Western blot. To obtain dihaploid homozygous plants of spring barley, variety Golden Promise containing a phytoremediation gene bphC/His was used anther cultures technique. In the thesis were choosed plants from the T2 generation, showing the presence of the inserted gene to test the ability of transgenic plants to accumulate polychlorinated biphenyls.
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Multiplikace česneku kuchyňského (Allium sativum L.) v podmínkách in vitro
Palíšek, Ondřej
This thesis is focused on study of Allium sativum multiplication and the composition of cultivation media. The best elaborated method is the multiplication of isolated apical meristem. Many different kinds of media have been tried, the results implies that each variety of garlic has different requirements for nutrients and growth regulators. In this work we can find descriptions of successful multiplication methods of and descriptions of used media, including the response of garlic to them. In vitro plant micropropagation represents a special way of propagation, when in controlled sterile conditions there are disinfected parts of plants (explants) cultivated on a nutrient medium to obtain a whole new plant or sanitation of plant material from pathogens. This method of propagation is used for study of plants and more often for commercial plant growing.
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Construction and evaluation of in vitro set of holes for chondrogenic differentiation of MSC cells
Kříž, Marek ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
Bachelor thesis concentrates on the cultivation of mesenchymal cells in vitro, damage to the articular cartilage and its replacement with the chondrogenic cells. The next section is devoted to each type of temperature control for heating and maintaining the temperature inside a closed system, the type of detector temperature and heating using the Peltier element. Assembly heater box, measuring the temperature profile inside the box with Labview program, control with Arduino platform, survival and differentiation of cells.
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Construction and evaluation of in vitro set of holes for chondrogenic differentiation of MSC cells
Kříž, Marek ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
Bachelor thesis concentrates on the cultivation of mesenchymal cells in vitro, damage to the articular cartilage and its replacement with the chondrogenic cells. The next section is devoted to each type of temperature control for heating and maintaining the temperature inside a closed system, the type of detector temperature and heating using the Peltier element. Assembly heater box, measuring the temperature profile inside the box with Labview program, control with Arduino platform, survival and differentiation of cells.
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Studium faktorů ovlivňujících mikropropagaci oskeruše (Sorbus domestica L.)
Mášlová, Tamara
This diploma thesis was elaborated on the theme "The study of factors influencing service tree (Sorbus domestica L.) micropropagation". The experiments were carried out in in vitro laboratory of the Mendeleum - Institute of Genetics and Plant Breeding of the Faculty of Horticulture in Lednice. The aim of this work was to acquire and improve the knowledge of in vitro cultivation of serviceberry and the genus Sorbus L. in general. The trees growing in experimental land of the Faculty of Horticulture MZLU in Lednice were selected as experimental material. The composition of cultivation medium was verified for plant growth and multiplication purposes. The influence of two different cytokinins BA (benzyl-adenine) and zeatin was evaluated. The shoot number and shoot length were measured. The highest multiplication rate evaluated by the origin of new formed shoots was accomplished on media supplemented with BA (0.1 mg.l-1). Nevertheless the shortest height of shoots was measured on this media. It was valid for both cytokinins that number of shoots was increasing with increasing cytokinin concentration whilst the shoot height was decreasing.
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Studium složení kultivačních médií pro množení odrůd meruněk metodou in vitro
Kadlecová, Eliška
In vitro reproduction represents a vegetative way of multiplication, in which small disinfected pieces of plant (explants) are cultivated in a nutritious medium in sterile conditions in order to gain whole new plants. This method, also called micropropagation, is often used for research purposes and more and more often for commercial applications too. This work studies nutritious media for apricots. For Prunus armeniaca cultivars best described media are for bud and embryo cultures. Many different media were tested and it was shown that every cultivar of apricot has its own requirements of nutrition and growth regulators. In this work you can find descriptions of many successfuly used media for apricots along with responses of various cultivars to them.
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Cultivation of grapevine in conditions in vitro
Faltus, Miloš ; Skala, Ondřej ; Bilavčík, Alois ; Zámečník, Jiří
Grapevine is a traditional crop in the Czech Republic, especially in the South Moravia region. It is a vegetatively propagated crop; hence vineyards are endangered by abiotic and biotic stress influence. The risk of plant damage due to frost and pest influence occurs in the field conditions according to season and locality. Cultivation of grapevine in conditions in vitro eliminates the risk. Higher utilization of the method is limited by sensitivity of some genotype to in vitro conditions. This methodology solves these problems and describes material introduction into in vitro conditions, explants multiplication and slow growth condition establishment. The Ministry of Agriculture of the Czech Republic is the user of this methodology and it will utilize it in the framework of “National Programme on Conservation and Utilization of Plant, Animal and Microbial Genetic Resources for Food and Agriculture”.
Fulltext:  PDF
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The issue of transfer selected hybrids of the orchids genera, grown under aseptic conditions in vitro, into non-sterile conditions
KELÍŠEK, Michal
In this paper experiments were made with three taxa of orchids. The first part was to find out whether the treatment of plants with potassium permanganate (KMnO4) has any impact on mortality during the transfer from in vitro culture to the unsterile environment of routine cultivation. All three taxa were divided into two parts and only one part was always treated while the other not. The deaths in individual groups were recorded during the experiment. In the second part the effect of fertilization on the biomass growth was investigated on two taxa. The method was again based on the division of the taxa into two parts and the fertilization of only one part. Necessary data were obtained by the weighing of plants at the beginning and end of the experiment. The results of the first experiment indicate that treatment of plants during the transfer has no impact on the subsequent mortality. In the second part, the dependence of fertilization on the biomass growth has been successfully proven in only one of the two taxa.
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