Home > Search Results: keywords:"reactive oxygen species"
Search:
Search Tips :: Advanced Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 63 records found  beginprevious61 - 63  jump to record: Search took 0.01 seconds. 
The study of photodynamic phenomenon on melanom cell lines
 KOLÁČKOVÁ, Zdeňka
Photodynamic therapy (PDT) is a medical method combining the use of photodynamic active substance and light in presence of oxygen. It extends the treatment possibilities of tumor and non-tumor disorders. It complements surgical treatment, radiotherapy, chemotherapy and immunotherapy. An increased accumulation of photosensitive substance in pathological focus is the base of PDT. Subsequent irradiation by light of suitable wavelenght evokes photodynamic reactions leading to formation of reactive oxygen species and to biological answer leading to tumor cells damage. The final effect depends on sensitizer type, its concentration in target tissue and on used source of radiation. Aim of the thesis is to prove photodynamic properties of newly developed photodynamic active substance phtalocyanin CIAIPcS2 and possibilities of its usage to induce photodynamic phenomenon in melanom cells. Owing to absorption of light in sensitizer the formation of excited states happens, and then the excited form of sensitizer reacts directly with substrate. During this reaction free radicals of substrate form or transfer of energy from sentitizer to oxygen and formation of highly reactive singlet oxygen happen. Free radicals, especially radicals of lipid components of cell membranes, are the major cause of tumor destruction. Fruitfulness of PDT depends not only on type of sensitizer and level of oxygen in tumor cell but also on used light source. Luminiscence diodes (LEDs) were used as the source of light. Formation of ROS after PDT was detected with the help of fluorescent molecular probe CM-H2DCFDA on spectrofluorimeter Synergy HT and on fluorescent microscope Olympus IX 81 with image analysis. According to our results the production of ROS depends on concentration of sensitizer CIAIPcS2 and the radiation dose. We proved that the sensitizer CIAIPcS2 is suitable photodynamic active substance and evokes photodynamic phenomenon in tumor cells.
Detailed record
Reactive oxygen species in photodynamic therapy, test of hydroxyl radicals production in the presence of phthalocyanine photosenzitizers.
 HONDLÍK, Ondřej
The theoretical part of bachelor thesis is focused on photodynamic therapy treatment, exp. on new light sources and second-generation senzitizers, and on the reactive oxygen species and the methods for their determination and quantification. In the experimental part, results of hydroxyl radicals photochemical production in the aqueous solutions of five phthalocyanines are presented.
Detailed record
Nutraceutical impact of fermented products on human
 KORANDOVÁ, Eliška
This bachelor thesis deals with nutraceutical impact of fermented products on human immunity, on the state of oxidative stress and on the quality of life. It presents probiotics, prebiotics and synbiotics and their expected influence on human health.
Detailed record

National Repository of Grey Literature : 63 records found   beginprevious61 - 63  jump to record:
Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English