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Spermatological characters in Bothriocephalidea (Cestoda)
ŠÍPKOVÁ, Lenka
Spermiogenesis and ultrastructure of the spermatozoon of two bothriocephalidean cestodes, Oncodiscus sauridae and Senga sp., have been studied using transmission electron microscopy. The presence of a classical pattern for spermatological characters (spermiogenesis of type I with dense-material in early stages and sperm of type II with a characteristic ring of cortical microtubules in the anterior part) in Bothriocephalidea is discussed.
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The use of negatve staining method for viral and bacterial diagnostics using the transmission electron microscope.
ŠOBEROVÁ, Tereza
This bachelor thesis is focused on the negative staining method used in making diagnosis of viruses and bacteria in the transmission electrom microscope (TEM). Theoretical part provides general description of TEM, negative staining method and basic characteristics of viruses used in this project. Detailed description of procedure designed for the preparation of specimens is the key part of this project. After their display in TEM, the testis analyses the quality of virus images and comparison of effects of the most common staining agents, pH, trehalose and different times of staining on displaying the ultrastructure of biological specimens and on the size of individual viral particles. The conclusion of project contains staining agents providing the best imaging of objects that have been selected based on the evaluated results and recommendations for potential enhancement of staining properties in other agents.
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Prospects of the scanning low energy electron microscopy in materials science
Mikmeková, Šárka ; Hovorka, Miloš ; Konvalina, Ivo ; Müllerová, Ilona ; Frank, Luděk
The use of the scanning low energy electron microscopy (SLEEM) has been slowly making its way into the field of materials science, hampered not by limitations in the technique but rather by the relative scarcity of these instruments in research institutes and laboratories. Various techniques exist which are capable of studying the material microstructure, with the scanning electron microscopy (SEM), (scanning) transmission microscopy ((S)TEM) and focused ion beam (FIB) microscopy being perhaps the most known. A specific way to visualizing the microstructure of materials at high spatial resolution, to achieve a high contrast between grains in polycrystals and very fast data acquisition is to use the cathode lens (CL) mode in SEM. The CL mode in the SEM enables us to detect slow but not only slow, high angle scattered electrons that carry mainly crystallographic contrast based on the electron channeling, mostly in the Mott scattering angular range.
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The first experience with a low voltage electron microscope
Nebesářová, Jana
The low voltage electron microscope LV EM 5 manufactured by Delong Instruments is described and methods of preparation of biological specimens for the observation in this microscope are briefly evaluated. LV EM 5 is a unique apparatus that could replace in medical and biological research laboratories high volatage transmission electron microscopes.
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