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Effect of sodium sulfide on the propreties of model globine-coupled heme-containing sensor proteins
Bartošová, Martina ; Martínková, Markéta (advisor) ; Man, Petr (referee)
Hydrogen sulfide mediates various physiological functions and along with carbon monoxide and nitric oxide it is an important gaseous signaling molecule. Cellular targets for H2S are proteins, enzymes, transcriptional factors or ion channels. In many cases, the effect of H2S on the regulatory protein is mediated by modifications of its cystein residues. In hemeproteins, the regulation of catalytic activity is induced by formation of the Fe(III)-SH complex or by reduction of the heme iron with subsequent formation of Fe(II)-O2 complex. The effect of Na2S on model sensor heme-containing proteins is presented in this thesis. Protein, isolated from bacterium Anaeromyxobacter sp. strain FW109-5, containing a globine coupled sensor domain and a histidine kinase domain is one of the studied proteins, the second one is protein isolated from bacterium Escherichie coli, containing a globine coupled sensor domain and a diguanylate cyclase domain. The effect of Na2S on both model proteins and their mutants was studied by UV-Vis spectral analysis. Spectra of YddV-HD Y43A were very unique, because thery confirmed formation of a homogenous complex Fe(III)-SH in this protein, whereas only mixtures of varous heme complexes were detected in other tested proteins. Additionally the effect of Na2S on functional domain...
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Ecological survey and assessment of streams within European Standards. Application in the Rolava River basin.
Beranová, Zuzana ; Matoušková, Milada (advisor) ; Kliment, Zdeněk (referee)
This thesis deals with implementation of Water Framework Directive (2000/60/EC) in the Czech Republic. The study area was the Rolava River basin in the Ore Mountains (northwest of Bohemia). The ecological status assessment was applied to both - the main stream and relevant tributaries. Available hydrobiological and hydrochemical data enhanced with the field hydromorphological survey using the Hydroecological monitoring (HEM, Langhammer, 2007) were applied in this thesis. Results of the field survey were compared with two ecomorphological methods: LAWA-FS (LAWA, 2000) and EcoRivHab (Matoušková, 2003, 2007). As a final result, it was found out the water body on upper course of the Rolava River achieved good ecological status. Water body on lower course of the Rolava River and of the tributary the Nejdecký Brook were classified as not achieving good ecological status. The Rolava catchment has very specific conditions and huge potential to achieve the good status in the future.
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Important roles of heme as a signal and a gas-sensing site: heme-sensing and gas-sensing proteins
Fojtíková, Veronika ; Martínková, Markéta (advisor) ; Hudeček, Jiří (referee)
Heme-containing sensor proteins are heme proteins, which are divided into two groups: heme-sensing and gas-sensing proteins. The function of heme-sensing proteins is affected by heme availability. Association (or dissociation) of heme moiety of heme-sensing protein regulates various physiological functions, including protein kinase activity, transcription and other important functions essential for cell survival. In gas-sensing proteins, heme acts as the sensing site for binding of gaseous molecules (including O2, NO and CO) and indirectly regulates physiological functions, including protein kinase activity, transcription and other important functions essential for cell survival. The recent studies on heme-containing senzor proteins published in scientific journals are summarized in this thesis. The experimental part of this thesis focused on the specific heme-containing sensor protein - a globin-coupled histidine kinase from Anaeromyxobacter sp. strain Fw 109-5 (AfGcHK). The aim of this thesis was to amplified and isolate plasmid carrying gen for AfGcHK. Consequently the protein was expressed in E.coli BL-21(DE3) and the protein was isolated. Based on the results, the isolation process was optimized. Moreover, the purified preparation of isolated AfGcHK was prepared in more than 99% of homogeneity....
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Hydromorphological survey of the Bilina River
Kyselka, Jan ; Matoušková, Milada (advisor) ; Langhammer, Jakub (referee)
The main aim of this thesis is assessment and presentation of results of the hydromorphological survey of the Bílina river, which belongs to rivers with the biggest anthropogenic impact in the Czech Republic. The method Hydroecological monitoring (HEM, Langhammer, 2007) was used for the survey. This method is compatible with the Water Framework Directive 2000/60/EC. 53 parts of the river with the lenght 31,99 km were assessed. The thesis also includes assessement of the rainoff regime and information about protection of water ecosystems and sources in the Bílina river basin. Keywords: hydromorphology, HEM, Bílina river
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Proposal of restoration measures in Slubice River basin based on ecohydromorphological survey
Šmerousová, Kateřina ; Matoušková, Milada (advisor) ; Just, Tomáš (referee)
This diploma thesis deals with ecohydrological state of the streams as an answer river habitat quality to exploitation, regulation and otherwise modification (flood-defence engineering, impoundment, river restoration, climate change or the spread of alien species), and with revitalization of water bodies for the purpose of elimination and mitigation of the negative consequences river ecosystem modification and improvement of ecological function river ecosystems in landscape. Focus is on the Slubice river basin in Landscape Protected Area Žďárské vrchy where field survey ecomorphological methods EcoRivHab a HEM was applied. On the basis of the obtained outputs antropogenic transformation of the river network, water quality, hydrological condition, definition of the reference state and assessment of the river patterns and changes of the length on the basis of the historical maps and ecomorphological survey outputs in the area of interest strongly antropogenic changed watercourse reaches was identified which require restoration and suitable restoration measures was proposed to the purpose of WFD goals attaining.
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Influence of dietary components and redox enzymes on intestinal microbiota proliferation in the tick \kur{Ixodes ricinus}
KUČERA, Matěj
In this work, we have analysed the temporal dynamics of gut-dwelling bacteria and Borrelia in the gut of the deer tick Ixodes ricinus. Using quantitative PCR, we have shown that levels of the tick intestinal microflora are profoundly decreased at later stages of feeding on whole blood but not on serum. Even though we noted that host complement system manages to interfere with Borrelia viability in vitro, we did not see any effect of host complement on Borrelia acquisition in adult ticks in vivo. However, we revealed that host hemoglobin is essential for Borrelia proliferation in the tick gut. All together, these data imply that, during feeding, levels of gut-dwelling bacteria and Borrelia are determined by the host. While hemoglobin seems to be detrimental for gut-dwelling bacteria, Borrelia require it in order to proliferate. During off-host stage, we showed that levels of gut-dwelling microflora are regulated by an intestinal transmembrane enzyme Dual oxidase. In conclusion, we aimed, and mostly succeeded, to perform pilot experiments describing the biology of a complex process of regulating gut microflora in the vector Ixodes ricinus and extend it by its impact on Borrelia acquisition
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Evolution of selected enzymes of the shikimate pathway and the haem biosynthetic pathway in Rhodophyta (class Florideophyceae)
VORÁČOVÁ, Kateřina
Diatoms derived their plastid from red algae through the secondary endosymbiosis. Most of the endosymbiont genes have been transferred from the engulfed alga to the secondary host nucleus, therefore evolution of these genes correspond to the evolution of plastids rather than to the evolution of the host organisms. Similarly, genes coding for ferrochelatase and DAHP synthase from diatoms are closely related to those from plants and green algae. Contrary to this, red algal genes do not cluster within this clade. I tried to amplify and sequence genes coding for ferrochelatase and DAHP synthase from representatives of the class Florideophyceae to investigate their phylogenetic position.
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