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Influence of antiparasitic baths of fish on haematological and biochemical indicators
MECKO, Jozef
The aim of the diploma thesis was to evaluate the influence of antiparasitic baths on selected biochemical and haematological parameters in the blood samples of fish. According to the results the application schema will be specified or amended such alterations will be substantiated. A test was performed on the fry of carp (naked and scaly form) to evaluate the influence of the peractetic acid with the help of biochemical and haematological parameters and with the histopathological examination. The common carp was exposed to the concetration 1 mg?l-1 and 2 mg?l-1 of PAA. Three days after application was taken the blood samples from fish and the biochemical and haematological parameters were determined. Than was taken a sample of skin with the musculature from fish to be sent for the histopathological examination in University of Veterinary and Pharmaceutical Sciences in Brno. Based on the results the naked form of the carp has been declared to be more sensitive to the application of PAA. All the observed changes in haematological and biochemical parameters were within physiological range, so it can be stated, that the application schema of PAA has not a signifiant negative influence on the health of the tested fish.
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Iodine content in milk and urine of dairy cows
VÍTKOVÁ, Lucie
This paper provides data on the iodine content in milk, urine and blood plasma of dairy cows on feed rations with various amounts and forms of iodine. The experiment included a group of 9 cows with different milk yield of livestock in the Klatovy district. The samples of milk, urine and plasma were collected from January to May 2011 at intervals of 3-4 weeks. Iodine content was determined spectrometrically after alkali burning of the sample. Vitamix S7+ mineral feed additives with anorganic iodine content and UNI SATURAN J with content of organic iodine were used as the sources of iodine. When feeding rations fortified with only anorganic iodine in quantity of 80 mg.kg-1 mineral feed additives, the content of iodine in milk was 33.07 +-31.13 mikrogramů.l-1, in urine 192.87 +-65.56 mikrogramů.l-1 and plasma 76.18 +-26.22 mikrogramů.l-1. When feeding diets with added organic forms of iodine in quantities of 1 mg.kg-1 mineral feed additives and lower contents of inorganic iodine its average content in milk reached 51.13 +-26.29 mikrogramů.l-1 in urine 221.20 +-137.65 mikrogramů.l-1 and 83.13 +-44.24 mikrogramů.l-1 in blood plasma. Low iodine content in milk and increased iodine content in urine indicate reduced use of iodine in the oraganism despite its optimum intake. These conditions are often associated with the effect of goitrogenic substances. Normal levels of iodine were reached in plasma.
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Effect of intrauterine autoplasma application on mare fertility
ŠTĚRBOVÁ, Hana
The objective of this study was to evaluate the effect of intrauterine plasma infusion on conceiving rate in selected group of mares during the breeding seasons 2008 and 2009. The fresh sperm insemination was followed by the infusion of self-plasma and subsequently the successivity of the insemination was compared with the control group of mares. Next, there were observed following factors which could influence the effectiveness of conceiving: age of the mares, number of insemination doses, size and location of preovulating follicle, month of the year. There was also evaluated the difference between multiparous (with one and more births) and nulliparous mares. The statistical analyses were proceeded by the use of contingency tables and the one-factorial analysis of variance (ANOVA). Next, the regression and correlation analyses were used. All the statistical procedures were performed with the use of statistical software STATGRAPHICS Plus 5.0. The results showed that there were the same results in observing of both number of insemination doses and fertile heats evaluation. There was found no significant effect of plasma infusion on the conceiving rate. In the model with the effects of plasma infusion and the age of the mare there were found statistically significant differences. It means that the effect of plasma infusion on conceiving rate was confirmed in the mares of the age up to 14 years. The result of the following analysis is the non-significant difference in conceiving rate between the groups of multiparous mares with or without plasma infusion.
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