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Diurnal changes of Rubisco activity under ambient and elevated concentrations of carbon dioxide
Uhrová, Lucie ; Vítová, Eva (referee) ; Hrstka, Miroslav (advisor)
Diurnal changes of Rubisco activity under the impact of ambient (380 µmol mol-1) and elevated (700 µmol mol-1) concentrations of carbon dioxide were studied in beech (Fagus sylvatica). Samples from leaves were taken in the two-hour intervals from 4:00 to 21:30 on the 22. 7. 2009 and activity of Rubisco was determined spectrophotometrically. No statistically significant differences were found between Rubisco activity of leaves growing in ambient and elevated CO2 concentrations. No increase of Rubisco total activity after daylight shows that nocturnal inhibitor CA1P is not present or plays only negligible role in beech. During the day Rubisco activity showed only statistically insignificant deviations. A slight decrease of Rubisco activation state in the afternoon indicates the afternoon depression of Rubisco initial activity.
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Influence of elevated carbon dioxide concentration at Rubisco activity and its content in sunny and shaded leaves of beech
Vičíková, Magda ; Vitoulová, Eva (referee) ; Hrstka, Miroslav (advisor)
This bachelor thesis focuses on influence of elevated carbon dioxide concentration at Rubisco activity and its content in sunny and shaded leaves of beech (Fagus sylvatica). The initial and total activities were measured spectrophotometrically, the content of Rubisco was determined by using SDS-PAGE method. It was observed statistically significant higher activity of Rubisco in leaves cultivated under elevated concentrations of carbon dioxide in June but not in September. In contrary, exposure of leaves (sunny/shaded) did not have significant influence on Rubisco activity in June, but in September was found lower activity of Rubisco in shaded leaves than in sunny leaves. The content of Rubisco was lower in leaves cultivated under elevated concentrations of carbon dioxide (acclimation), with the exception of shaded leaves in September. There was found significantly lower Rubisco content in shaded leaves than in sunny leaves both in June and in September.
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Diversity of ectomycorrhizal fungi in selected beech stands in Blanský les
HEJNA, Ondřej
The diversity and abundance of ectomycorrhizal fungi in three beech stands were compared during 2012 and 2013 years in two soil horizons. Individual ectomycorrhizal roots tips were morphologically and anatomically characterized. Representative morphotypes were identified by using molecular methods to confirm and verify ectomycorrhizal fungal species.
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Diversity of ectomycorrhizal fungi in beech forest \kur{(Fagus sylvatica)}
HEJNA, Ondřej
The objectives of this work described here were to summarize and evaluate current knowledge in ectomycorrhizal fungi with the intention at the colonization of root system beech Fagus sylvatica to ectomycorrhizal fungi. This work mainly was targeted at diversity of these ectomycorrhizal fungi in beech forest affected positive and negative by environmental and anthropogenic factors. Last part of this work describes qualitative and quantitative methods used in investigation of ectomycorrhizal fungi and their diversity.
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Seasonal variability in isotopic signature of leaf water and related water compartments. An ecophysiological perspective.
PLAVCOVÁ, Lenka
Stable isotope composition of bulk leaf water is a useful proxy for geochemical and biological processes and is, thus, appreciated in ecological research and global modelling. The mechanisms determining the abundance of different isotopes in leaf water are not fully understood. The research presented in this thesis aimed to describe variability in leaf water isotopes which occurs under natural conditions and to distinguish which factors generate such variability. For that purpose, field sampling, water extraction, stable isotope analysis and measurements of several additional environmental and physiological characteristics were carried out at one sampling site during three subsequent growing seasons. The results obtained point out that oxygen isotopes rather then hydrogen isotopes have greater potential to be used in higher scale applications. Apart from that, several interesting ecophysiological interpretations of isotopic data have been made.
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