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Detection of phytoplasmas using PCR amplification of selected fragments from the phytoplasma genome
MARKOVÁ, Jaroslava
Phytoplasmas are bacterial pathogens of plants. They can cause devastating losses of yield of diverse crops. It is necessary to find reliable detection technique for detection of phytoplasmas disease. The easiest and the cheapest technique is just PCR (polymerase chain reaction) which is used to selective amplification of selected parts of the molecule DNA. The aim of this bachelor thesis is to find of optimal primers to amplification of selected fragments of phytoplasmas genome in one-step PCR and use this technique in detection of phytoplasmas. A collection of phytoplasmas from professor Bertaccini with 37 samples was used for this study. It was tested 16 natural samples by optimized primers. Phytoplasma was detected in strawberry root. Research was supported by the Czech Science Foundation, Grant No. GP 522/09/P545.
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Development and optimalization of methodology for detection of GMO potatoes
ČERMÁKOVÁ, Jitka
Genetically modified (GM) or transgenic crops, now more often called ``Biotech crops{\crqq} they are commercially cultivated since 1996. And also since 1996, the first year of commercialization of biotech crops, GM potatoes were cultivated in USA, Mexico, Canada and later in South Africa, China and India. The global area of approved biotech crops in 2006 was 102 million hectares and 22 countries grew biotech crops, 11 developing countries and 11 industrial countries. The Czech Republic is on of the six EU countries where biotech crops are cultivated at present. The most compelling case for biotechnology, and more specifically biotech crops, is their capability to contribute to: increasing crop productivity and stability of productivity and production; conserving biodiversity, as a land-saving technology; the production of renewable resource based bio-fuels. This diploma paper was focused on developing of fast, precise and cheap method based on PCR to detect the presence of transgenes in potatoes {--} tubers and leaves, allows monitoring the presence of GM potatoes in market, environment, etc. and to quantify ``contamination{\crqq} of ware potatoes (tubers) with GM ones.
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