National Repository of Grey Literature 166 records found  beginprevious165 - 166  jump to record: Search took 0.00 seconds. 
The evaluation of horse nutrition with chosen preparats in sommer and winter season
HANETŠLÉGROVÁ, Petra
ABSTRACT The aim of this work was to provide a detailed breakdown of horse nutrition, to mention the importance of dietary supplements. The main part deals with the basics of horse nutrition, the bare necessities of nutrients, the proportion of maintenance rations and the technique of feeding. Afterwards, I outline the properties and effects of varied herbal plants such as garlic, hemp, hyssop, white horehound, coltsfoot and oats. Flaxseed, biotin, zinc, yeast and probiotics are dietary supplements improving the quality of fur, horsehair and hoofs. The feed will be analysed by standard methods. As far as horse excrement is concerned, we are going to observe its shape, colour, consistency, pH value and smell. The assessment of horse excrement will serve as a judgement about the activity of digestive system. The ethology observations will provide information on daily activity of a horse, on particular activities, to which the horse gives its attention during a day, and the amount of time it dedicates to them. As far as horses with respiratory disorders are concerned, we will observe the signs of their health problems and the rate of these problems occurring both at rest and in action. The information will be completed by the measurement of body proportions useful to body weight calculation. The statistical and economic methods will be selected in terms of the results accomplished during the research.
Interactions of Zn2+ ions with proteins and nucleic acids
DVOŘÁKOVÁ, Kateřina
Interaction of Zn2+ ions with proteins and nucleic acids Zinc is an important metal in biological systems. It is a strong Lewis acid, forms a stable Zn2+ ion and can exist in several coordination geometries. Zinc is required for the activity of more than 300 enzymes. In proteins zinc can either participate directly in chemical catalysis or can be important for maintaining protein structure and stability. In this work a dataset of high quality (resolution better than 3 Ǻ) crystal structures deposited in the Protein Data Bank on internet have been examined to identify typical zinc binding sites and to establish their coordination geometries. Totally 135 zinc binding sites found in 74 structures have been analyzed. The most frequent coordinating atoms are sulphur in cystein, NE2 and ND1 nitrogens in histidine followed by oxygen atoms in carboxylates of aspartic and glutamic acids. 67% of zinc binding sites were found to be four-coordinated with tetrahedral coordination geometry. In contrast Mg2+ cations show a clear preference to bind to oxygen atoms and to form octahedral cavities. We have not found any structure with a direct zinc coordination to DNA except the 1ZQT structure which was however excluded due to its low resolution (> 3 {\AA}). Similarly only three zinc binding sites have been found in RNA structures which were however determined in the protein free environment. All other zinc binding sites have been located in proteins even in the presence of the nucleic acids.

National Repository of Grey Literature : 166 records found   beginprevious165 - 166  jump to record:
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