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Biotribology of artificial tears for the treatment of dry eye syndrome
Černohlávek, Mikuláš ; Daniel, Matej (referee) ; Vrbka, Martin (advisor)
This master thesis is a part of the project TAČR TREND, which aim is the development of new ingredient for treatment of dry eye syndrome. Developed ingredient is derivative of hyaluronic acid, which is in this thesis compared with native hyaluronic acids and commercial eye drops. Aim of this thesis is to describe rheological and tribological properties of measured samples in terms of viscosity, mucoadhesivity and coefficient of friction. Coefficient of friction was measured on created tribological model of the eye in pin-on-plate configuration which represents physiologically relevant sliding speeds of blinking. Comparison with native hyaluronic acids and commercial products of eye drops showed, that derivative reaches higher values of viscosity, has mucoahesive properties and has low values of coefficient of friction. Those key parameters show that modification of hyaluronic acid is promising way for drug development for dry eye disease.
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Lubrication of knee joint replacement
Sadecká, Kateřina ; Horák, Zdeněk (referee) ; Vrbka, Martin (advisor)
The work deals with the lubrication of total knee replacement using fluorescence microscopy method, which allows unique insight into the contact between femoral and tibial component. The aim was to determine the effect of composition of synovial fluid (i.e. albumin, -globulin, hyaluronic acid and phospholipids) on film thickness and protein behaviour in contact, and also to determine changes of contact area during rotation. Since this is the first experimental work dealing with a knee replacement lubrication primarily, only simple rotation and load cycles were applied by the knee simulator. The output of the experiments was fluorescence intensity, which corresponds to dimensionless film thickness, over time. Another important output are the images directly showing the fluorescently labelled proteins in the contact area. The results show, there are fundamental differences in lubrication in different positions of rotation, due to changes of position, shape and behaviour of the contact area. The composition of the lubricant is also essential, since the proteins themselves form a relatively strong lubricating film and their mixture leads to a substantial reduction of film thickness, due to significant formation of clusters. Complex fluid, although it does not form the strongest layer, is able to create a quite continuous film.
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Experimental study of friction in articular cartilage
Praus, Tomáš ; Vrbka, Martin (referee) ; Rebenda, David (advisor)
This thesis deals with the experimental study of friction in articular cartilage. After the introduction to biotribology, there are described a principle of measurment on the Mini-Traction Machine and a brief description of machine itself. After that, there is a propose for a preparation and storage of cartilage samples, construction of the adapter into the MTM and research of the friction coefficient. The results of experimental research showed the negative effect of storing the samples in the refrigerator. On the other hand, the measurement of the friction coefficient of the samples stored in the freezer showed the preservation of the mechanical properties of cartilage. Even load and speed of loading have a big effect on friction. When the speed increases, the coefficient of the friction increases too. There is an extreme increase of the friction at higher speeds. But with increasing load the friction coefficient decreases.
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The Effect of Synovial Fluid Constituents on Lubrication of Hip Joint Replacements
Nečas, David ; Daniel,, Matej (referee) ; Sawae, Yoshinori (referee) ; Hartl, Martin (advisor)
Dizertační práce se zabývá mechanismy mazání v náhradách kyčelního kloubu. Byla provedena systematická studie formování proteinového filmu při zahrnutí různých materiálů a provozních podmínek. Hlavní pozornost je přitom věnována roli jednotlivých proteinů obsažených v synoviální kapalině při současné přítomnosti dalších proteinů. Jelikož metody aplikované v předchozích studiích neumožňovaly separovat jednotlivé složky maziva, byla vyvinuta optická měřící metoda na principu fluorescenční mikroskopie. Z důvodu verifikace metody byly provedeny dvě nezávislé studie zaměřené na měření tloušťky mazacího filmu a dělení maziva na výstupu mazaného kontaktu. Z důvodu určitých limitací fluorescenční mikroskopie byla dále využita i metoda optické interferometrie, jejíž využití je ilustrováno ve studii zabývající se formováním mazacího filmu v náhradách kyčelního klubu při uvažování reálné konformity třecích povrchů. Závěrečná část práce představuje nový metodologický přístup založený na in situ pozorování kontaktní oblasti umožňující popsat roli jednotlivých proteinů ve vztahu k vývoji tloušťky mazacího filmu. Práce obsahuje originální výsledky, které přináší nové poznání v oblasti biotribologie náhrad kyčelního kloubu vedoucí k dalšímu vývoji implantátů při snaze zabránit jejich selhání v důsledku omezené životnosti.
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Study of Friction in Hip Joint Replacements
Balounová, Hana ; Dzimko, Marián (referee) ; Vrbka, Martin (advisor)
Diploma thesis deals with analysis of coefficient of friction in total hip prosthesis for several materials bearing with presence of bovine serum as substitute of synovial fluid occurred in natural joint. Behavior of coefficient of friction is observed on Mini Traction Machine. Results are plotted at graphs representing dependence of coefficient of friction on time. There are described effects of several kinematic conditions, the influence of used material and the effect of the method of contact lubrication. The experiments analyze how the formation of lubricant film with a layer of adsorbed protein affects coefficient of friction.
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Mapping of lubricating film thickness of human big joints replacements
Bosák, Dalibor ; Hartl, Martin (referee) ; Vrbka, Martin (advisor)
This master’s thesis describes mechanism of lubricating film forming and its thickness in contact of a metal hip joint ball and a glass disc with chromium layer with presence of bovine serum as substitute of synovial fluid occured in a natural joint. Lubricating film thickness is evaluated by a thin film colorimetric interferometry, results are ploted at graphs representing dependence of lubricating film thickness on experiment duration or on mutual speed of articulating surfaces. There is described the influence of concentration of bovine serum and different kinematic conditions on lubricating film forming. From observed interferograms is apparent the adsorption of proteins to glass disc and femoral head surfaces, denaturation and formation of protein aggregations affecting the residual lubricating film thickness.
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Tribology of hydrogels for articular cartilage replacement
Křížová, Michaela ; Ranuša, Matúš (referee) ; Vrbka, Martin (advisor)
The bachelor's thesis deals with the description of the tribological properties of focal joint implants in the place of a cartilage defect. The application of focal implants is aimed at a group of younger patients who have to undergo surgery to repair damaged cartilage, but a total replacement is not suitable for them due to its lifetime. The contact of the focal implant and the opposite cartilage was simulated using metal samples and hydrogel, which has very similar properties to natural cartilage. For a possible comparison of values, a tribological measurement of the cartilage samples against the hydrogel was carried out. This pair serves as a model for defining friction in a healthy joint. The main task of this thesis was to determine the coefficient of friction of Ti6Al4V, Ti6Al4V+DLC, CoCrMo and cartilage against the hydrogel. The experiments were carried out using a tribometer in a pin-on-plate configuration. The Ti6Al4V alloy without coating and with DLC coating came out with the lowest coefficient of friction for both variants, almost identically 0.0526. Higher friction was measured for CoCrMo, but it differed from the titanium alloy to one-hundredth. On the contrary, the highest coefficient of friction was unexpectedly manifested in the cartilage samples, where the lowest friction was assumed. The error was due to poor selection of cartilage samples. The thesis simply describes the tribological behaviour of focal implants and the result is a material with the lowest coefficient of friction. The measurements performed could help further research into the issue of focal implants.
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Friction investigation of coated materials for joint implants
Gelnar, Adam ; Streďanská, Alexandra (referee) ; Nečas, David (advisor)
This bachelor thesis deals with the comparison of the coefficient of friction of different types of DLC coatings applied on two materials used for joint replacements. The experiments also aim to compare the friction as a function of the material pair combination. The friction tests were performed on a Bruker UMT TriboLab tribometer in a pin-on-plate configuration. The surface topography before and after the tests were also analyzed. The measured results show that the pins have lower friction coefficient values against the UHMWPE plate than against the glass. The uncoated samples, although having lower friction values, showed some signs of wear after the tests, while the pins with DLC coatings did not. In view of these findings, it can therefore be assumed that surface modification of commonly used materials with DLC coatings can lead to an extension of the lifetime of joint replacements, and therefore to significant advances in implantology.
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Friction and lubrication of fascia
Chmelík, Jiří ; Daniel, Matej (referee) ; Vrbka, Martin (advisor)
The diploma thesis was part of the project "Regeneration and lubrication of fascia with hyaluronan" of the company Contipro a.s., whose main goal is the development of an injectable medicinal product. The number of patients suffering from so-called myofascial lower back pain is increasing. The cause of the pain is increased hyaluronic acid, which is produced between the layers of the fascia. If the viscosity is low, the fascia layers are not affected and the coefficient of friction is low. The purpose of the product is to improve the sliding movement of the fascia and relieve patients of pain. The main component of the medicine is hyaluronic acid. The project included rheological and tribological analysis of hyaluronic acid samples. A tribological model of fascia in a pin-on-plate configuration was created for the analysis of the coefficient of friction. Based on the fascia models used, the dependence between the coefficient of friction and viscosity of native hyaluronic acid solutions was found. For models and real fascias, the native solution (Bonharen) was found to have better friction properties than hyaluronic acid derivatives. These analyzes have contributed not only to the development of the drug, but also to the emergence of other studies that would like to address the biotribology of fascia.
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Effect of toothpaste abrasiveness on friction and wear of dental fillings
Formánková, Pavla ; Čípek, Pavel (referee) ; Vrbka, Martin (advisor)
This bachelor thesis dealt with research, that links dental hygiene issues with tribology. The aim of the thesis was to analyze the effect of abrasive particles of toothpastes on friction and wear of dental fillings. The friction coefficient was evaluated in a tooth brushing simulation on an UMT TriboLab tribometer that lasted 260 minutes. The tribological system consisted of a toothbrush, a sample of composite material and a solution of artificial saliva with a selected toothpaste. Each paste was defined by an RDA value from 0 to 250, expressing the degree of abrasiveness therefore the ability of the toothpastes to wear the material. Wear was analysed on a Bruker Contour GT-X optical profilometer. From the measured values it was found that the influence of abrasive particles is significant in terms of wear of dental fillings. For both the friction coefficient and the material loss when measured only in the presence of artificial saliva, the resulting values were generally lower. However, the material loss in the toothpastes measurments did not increase linearly with the RDA value as expected. Literature studies and measurements have shown that this may be due to other factors affecting wear. Future research on these factors could lead to the selection of more gentle toothpastes in dentistry and thus prevent the increase in wear of dental fillings
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