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Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Martínková, Ludmila ; Kaplan, Ondřej ; Veselá, Alicja Barbara
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. The enzymes differ from each other in substrate specificity, temperature optimum, enantioselectivity (arylacetonitrilases) etc. Preferential substrates are phenylacetonitrile, (R,S)-mandelonitrile, benzonitrile or cyanopyridines (depending on the enzyme).
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Music As A Sign
JAROLÍMOVÁ, Jana
The thesis is interested in the problem of musical sign. In which ways can we speak about musical sign and which elements of musical material can become signs? These are the basic questions which we are interested in. This thesis is divided into three parts. The first part attends to the common theory of sign. The historical survey with the main ideas of philosophers and thinkers will be helpful to find out basic terms and problems of semiotics. In the second part of thesis we look at the historical reflection of musical sign in order to find out in which way it was treated with music and musical sign in each historical period of European music. In the third part we focus on musical semiotics, but we will consider some problems of musical aesthetics and musical psychology, too. In the end we make recapitulation of answers to the aforesaid questions.
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Visual expression of emotions and the work upon human
BLÁHOVÁ, Lenka
The main theme of this Bachelor's thesis are emotions. The goal is to clarify this concept, and then apply it to the context of fine art. The work is divided into part of the theoretical and practical. In the part dealing with the theories has to be clarified the relationship of emotions and colors, but mainly the display of emotion through visual art. Further emphasis is placed on the impact of the work on the viewer emotional page. This phenomenon is analysed in chronological order from the beginning of mankind until the present. The practical part includes a triptych of paintings focusing on the topic of emotions, the description of the painting process, including the inspiration of resources and subsequent consideration of their effects.
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Expression and purification of \kur{Synechocystis} ferrochelatase from \kur{Escherichia coli}
RICHTOVÁ, Jitka
Ferrochelatase (FeCH) is an ubiquitous enzyme producing heme, an essential pigment for all forms of organisms. In photosynthetic organism, heme is synthesized together with the chlorophyll in one branched pathway and the FeCH enzyme appears to be important for regulation of both the chlorophyll and the heme biosynthesis. To understand regulatory role of this protein, an active recombinant FeCH from photosynthetic organism would be invaluable. The aim of this project is to express FeCH from cyanobacterium Synechocystis 6803 in Escherichia coli and to prepare a protocol for the purification of this protein as a highly active enzyme.
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