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Dynamics of mouse sperm capacitation and acrosome reaction
Dvořáková-Hortová, Kateřina ; Frolíková, Michaela ; Děd, Lukáš ; Šebková, Nataša
Capacitation followed by the acrosome reaction (AR), is a very complex event of molecular changes, including acrosome matrix rearrangement and actin polymerization, which mammalian sperm must undergo in the female reproductive tract in order to obtain the ability to penetrate and fertilize the egg. CD46 and β1-integrin belong to specific proteins, which are predicted to interact during molecular reorganization of capacitating sperm. The IZUMO1 as the primary fusion protein of the mammalian sperm is also involved in this dynamic network. We investigated the relationship between the Izumo, CD46 and β1 integrin relocation in the sperm head during the capacitation and AR in vitro. We have already successfully monitored by immunofluorescent labelling the dynamics of proteins CD46 and β1-integrin. The changes in the localization of these proteins associated with the AR and their mutual co-localization was observed. The original β1-integrin location in the freshly released epididymal sperm is in the acrosome and it relocates during the AR further through the sperm head compartments into the equatorial segment and over the whole sperm head. Its density over the equatorial segment is decreasing with the extended time of the AR. Also its presence in the perforatorium of the mouse sperm head is very prominent. The pattern for protein CD46 is extremely similar if not identical in both aspects such as compartment localization and time progress during capacitation and AR in vitro. The molecular interaction of CD46 and β1-integrin was investigated using the Proximity Ligation Assay and Super resolution microscopy STED. The data were statistically analysed. The newly obtained results from CD46 and β1-integrin relocation are in correlation with IZUMO1 dynamics and giving a substantial knowledge on the studied protein network rearrangement during capacitation and AR in mouse spermatozoa.
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International Digital Library of Manuscripts
Knoll, Adolf,
The Manuscriptorium Digital Library is Europe’s largest digital library of manuscripts providing access to data from more than 120 institutions across Europe incl. ca. 55 most important Czech collections. In March 2015, it contained 43,372 fully digitized documents and more than 337,000 bibliographic records. The documents are not only of European origin (Western, Slavonic, Hebrew, Armenian ...), but there are also data representing Arabic, Persian, Ottoman, Indian and other manuscript traditions. The digital library was launched in 2003 by the National Library of the Czech Republic, in partnership with the Czech AiP Beroun Company. In comparison and in contrast with other aggregation services, it provides seamless access to full digital documents on the fly, reusing thus repositories of partner digital libraries. Users do not need to travel or to navigate from one physical place or digital library to another one to access needed documents. Manuscriptorium provides uniform interface to any data from any digital library that signed the partnership agreement. The internal metadata solution is based on the robust TEI P5 approach and suits both library and researchers’ requirements for description and structuring. It aggregates not only fully digitized documents, but also the partially digitized ones and bibliographic records. It contains manuscripts as well as old printed books, historical maps, and historical posters. The documents are mostly represented by digital images and in certain extent also by full texts, TEI-structured and unstructured. The Manuscriptorium larger environment provides also online support to TEI authoring and online metadata validation and upload. ...
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Lifestyle of patients with peripheral arterial disease before and after surgery
REZKOVÁ, Helena
Theoretical background: Peripheral arterial disease is a serious disease of Loir limb arteries which originates from atherosclerosis. The theoretical part deals with the lifestyle. There are mentioned basic risk factors of chronic forms. Further described are the pathophysiology changes generated on the basis of atherosclerosis and related causes for the origin of the disease. Subsequently there are explained methods of classification, developed clinical symptoms, diagnosis and therapy. This section also looks at the role of a nurse in the care of patients with peripheral arterial disease. Objectives: The aim of this work was to evaluate how patients with peripheral arterial disease changed their lifestyle after invasive vascular surgery. There were provided the following research questions: How do patients with peripheral arterial disease approach to the prevention of risk factors? What is in patients with peripheral arterial disease the main impuls for the possible change of their lifestyle? What influences surgery patient's attitude to risk factors for peripheral arterial disease? How crucial is a lifestyle change in patients after surgery for peripheral arterial disease? Methods: The theoretical part is processed using expert sources. The empirical part of the theses was processed using qualitative research. The chosen technique for data collection was semi-structured interviews with patients, with prepared open questions. The research was done in several phases - the first interview was conducted at the patient admission department (before surgery), followed by the next phase after three months, and 6 months after surgery. The interviews were recorded on a prepared form, then transcribed into electronic form and analyzed by open coding method. Results: The research showed that surgery can sometimes be triggered by changes in the patient's lifestyle. The first interviews in the preoperative period focused on how respondents felt, what was their lifestyle before surgery for atherosclerotic changes of the lower extremities. One of the questions was focused on patient knowledge about risk factors for the development of PAD and possible prevention. The second phase of interviews took place 3 months after the surgery. It dealt with the question about the respndents´ lifestyle, the respondents in the postoperative period, whether there were any changes. Some positive changes occurred in eating and there were some changes in doing physical activity. The third stage was carried out six months after surgery, again in order to detect changes in lifestyle. Minor changes came in smoking habits, the issue of diet and physical aktivity. Questions were also focused on the psychological level. We found out who helped patients and what was most motivating in implementing changes. In the conclusion/summing up question the patients marked themselves ow they managed to make a change. Conclusion: The number of patients with peripheral arterial disease and other cardiovascular diseases is increasing. The basis is the start to modify risk factors, namely tobacco use,dietary habits, physical activity. The medical staff members participate on changes in patients´ lifestyle by appropriate education. The problem occurs when the patient despite the support of close people can not cope to carry a twist in their life. There should a medical staff member offer a professional help to deal with this important step. Practical use: The aim is to draw attention to the expansion of education about lifestyle changes for patients diagnosed with PAD also in the postoperative period. Patients who are released to home care should receive enough information that would convince them to change their attitude towards their health, and that surgery is only one of the ways to prevent further attacks and complications. Results can improve communication with patients and focus on increasing their awareness and possibly offer assistance.
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Card of Cryptosporidium infections by humans and animals
BLÁHOVEC, Ondřej
The representatives of the Cryptosporidium genus are one of the causes of gastrointestinal tract diseases in humans and animals. In animals the host may even be a respiratory apparatus. The disease is called cryptosproridiosis. In majority of cases this infection can bypass without any major problems, but in immunosuppressed individuals it can cause serious health problems. Cryptosporidium has a monoxenous development cycle, which basically means that the entire development takes place in a single host. Exogenous stage is represented by oocysts, which are in case of a gastrointestinal disease excreted in faeces. In case of a respiratory disease the oocysts make they way out via respiratory and nasal secretions. This leads to contamination of the environment or water. In general, it is expected that Cryptosporidium isolates, which are present in one class of vertebrates, are not infectious to a non-specific host from other classes. It is also expected that cryptosporidia have low host specificity. But this does not exclude that some kinds have gradually extended its specificity to more species. It is also apparent that cryptosporidium infections are common in animals that inhabit the external environment, so even a human can be endangered by this zoonosis, although the incidence in the Czech Republic is low. The reason for the low numbers may as well be that parasitological examination is not performed very often, so the estimated prevalence in the population is probably much higher. Therefore to reveal the originator of this disease it would be appropriate to perform a parasitological examination in persons who are in contact with animals, this way the cryptosporidium infection would be excluded or proven.
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Summary of foreign knowledge about the origin and development of EDZ in crystalline rocks - research
Vavro, Martin ; Souček, Kamil ; Staš, Lubomír ; Vavro, Leona
Presented search summarizes findings of foreign research oriented on the origin and evolution of the excavation damaged zone in crystalline rocks with a particular focus on the essential results of experimental projects which were performed in Canada, Sweden, Finland, and Switzerland. The study is divided, excluding the introduction, into seven main chapters of the text, which gradually deal with: (1) definition of key terms, (2) overview of the main underground research laboratories in the world where EDZ assessment was conducted, (3) methods suitable for EDZ description and characterization, (4) main factors influencing the origin of failure around the excavations and time-dependent evolution of EDZ. An overview of important outcomes of EDZ experiments, focusing on the European hard rock laboratories (Stripa, Äspö, Onkalo/Olkiluoto and Grimsel), and their summary are presented in the final two chapters.\nThe review summarizing the published key findings and results of in situ experiments shows, that for rock in lower stress state, i.e. in no spalling environment, the extent and character of rock mass damage is typically dependent on the excavation method. Using mechanical excavation, rock damage zone with thickness less than 3 centimeters can be originated. The microcracks within this zone contribute to the increase of hydraulic conductivity of the rock mass. At some test sites (Äspö, Grimsel), where the tunnel boring machine technology was used, the damage zone was already detected in depth of less than 5 mm.\nOn the contrary, openings excavated by drilling and blasting are characterized by much more extensive damage zones up to several tens of centimeters in width. The damage progressively diminishes with the distance from the opening.
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The waterpipe like a lifestyle: Origin and development of waterpipe smokers in Czech Republic
WEINFURT, David
This work is aimed at the formation and description of a subculture of people smoking water pipes in the Czech republic. It describes the characteristics of this subculture and using research based questionnaire method it describes who belongs to this subculture. The author then presents the reasons why is that so. A subject of water pipe smoking health risks is discussed in an individual chapter. Final section attempts to predict both future development of businesses where is it possible to enjoy a water pipe and the development of the whole water pipe smoking subculture in the Czech republic.
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Realization of farm with a focus on poultry
Hromjak, Milan ; Zita, Lukáš (advisor) ; Kovářová, Kateřina (referee)
This thesis deals with the origin of a family farm. By creating a business plan and issues connected with it, its priority is an organic poultry farm, which will produce poultry meat and eggs. Another service beyond the farm production will be creating conditions for a developing branch, which is agro-tourism and other services related to the operation of the farm, so that it leads to the economic development and profit.
The farm will arise by rebuilding of an old house with a large barn and adjacent 10ha of lands inherited from grandparents. There will be built a multi-purpose building called the poultry farm from which the poultry will have access to pasture. The property includes another 1 ha of forest from which the wood will be used as a building material and firewood.
The work is divided into two parts: theoretical and practical. The theoretical part especially focuses on explaining the concepts that are important to understand the business plan.
Then there is the legislation concerning the organic farming, a tangle of laws, public notices and regulations that must be followed in order to meet the conditions for the organic farming. An integral motivational service of the country and the European Union for farmers and not only for those who are environmentally-managing farmers, is support and grants for the years 2014 - 2020. A very important step not only for the starting farmers is a market analysis in the given branch, as well as a SWOT analysis that will show us the positive and negative aspects of the business. The conclusion of this section deals not only with the concept of agro-tourism, but also with its market position as well and possibilities of its use and further development.
In the practical part there is a description of a business plan and individual periods of the project including financing. Finances will flow from own and foreign capital, or the possibility to apply for support through non-refundable grants. The establishment of the farm will have an impact on the labor market, as there will be 5 newly created jobs and the community will fit into the wider public awareness thanks to the activities that the farm will develop for the purpose of product promotion and further development. Furthermore, there are itemized individual items that lead to the final evaluation of the effectiveness of the business plan. By processing the theoretical and practical part of the thesis the requirement of the thesis objective is met - to design a prosperous family farm focused on poultry keeping with a hypothesis if it is currently possible to be competitive while importing poultry meat and eggs from abroad. It can be concluded that the business plan is viable.
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Active employment policy in selected region
Čihák, Pavel ; Šrédl, Karel (advisor) ; Markéta, Markéta (referee)
The thesis considers unemployment and a possibility of increasing employment in the Central Bohemian Region using the active employment policy. The first part of the thesis is dedicated to theoretical basis gained from professional literature and internet resources about labor market, types of unemployment depending on the cause of its origin, risky groups of unemployed people, social and economic consequences of unemployment and about an analysis of actions of the active employment policy. Furthermore, there is described the Central Bohemian Region and its strong and weak aspects. We can look at the evaluation of employment and unemployment made by a study, status reports of the labor market and processing of statistical indicators in Central Bohemian Region. We can also peek at regional individual projects realized on the territory of Central Bohemian Region in 2015. The last part of this thesis is focused on some possibilities of more effective use of actions of the active employment policy in the region.
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Morphometric analyse of Vinca minor populations in the CZU university collection
Závorková, Hana ; Karlík, Petr (advisor) ; Černý, Tomáš (referee)
Lesser periwinkle (Vinca minor) is pretty common, perennial and woody plant, for centuries disseminated for her aesthetic and medicinal use. The aim of this thesis is to try to identify, if individual subjects disseminated throughout the Czech Republic may come from one or more sources or clones. It was collected a total of 135 individuals throughout the Czech Republic, and these individuals were grown for several years in uniform climatic conditions in campus of the Czech Agricultural University in Prague. For these plants were then performed a morphological measurements and statistical evaluation was performed by programs Statistica12 and Canoco5. From data obtained this way was revealed that the morphological features of individual plants are dependent on the type of site, and therefore it is clear that individuals grown on historically verifiable sites probably originate from one or several clones. Another aim of this work is to determine whether the periwinkle occurring in the Czech Republic differ in their morphological and physiological characteristics such as stem length, length and width of leaf, petiole length, number of nodes on the flowering stem and the ratio length / width of the leaf. The results implies the fact that individuals differ in morphological features depending on the location of their occurrence, such as parks, churches, cemeteries and forests. The results further shows to us that location no. 2 differs significantly from the other locations in the length of the stem, leaf length, number of nodes and in the ratio length / width of the leaf. This is confirmed by the results of the program as Statistica12 and the results of the program Canoco5.
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