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Comparison of bacteriological examination of urine using the cultivation and nephelometric method
LOJÍKOVÁ, Aneta
In population urinary tract infections (UTI) occur as the second most common diseases caused by various pathogens. They manifest themselves by the presence of bacteria in urine called bacteriuria. The objective of the thesis was to compare the bacteriological examination of urine in suspected UTI using cultivation-based methods and nephelometric methods. Two procedures were used for cultivation examination of urine. The first was a gradual dilution technique which is considered to be accurate. The second procedure was cultivation on filter paper which is considered to be less reliable. Two types of culture media - URI Select 4 and COS from the firm BIO-RAD were used.. URI Select 4 is a diagnostic medium designed to cultivate urine samples. Blood agar (COS) is a blood enriched medium on which the majority of medically important bakteria grow. The third measurement was carried out with the automatic system URO-QUICK from ALIFAX S.p.A. Cultivation on filter paper was used only as an extending supplementary method to be compared to the gradual dilution technique and the automatic system URO-QUICK. Total of 50 urine samples were tested.

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