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The occurrence of Coccidiosis in the intestine of sucking pigs before and after weaning
KOTILOVÁ, Jiřina
In two years of observation, (spring 2006, autumn 2006, spring 2007 and autumn 2007) were being screened for parasites in total 495 faecal samples coming out of three farms from Ceske Budejovice (285 samples of sucking pigs not older then 28 days and 174 samples of piglets not older then 8 weeks). The method used to examine those samples was a flotation-concentrating method (Sheather{\crq}s carbohydrate fusion) and in the year of 2007 was also used a specific aniline-carbol-methyl violet staining method to detect the Cryptosporidium spp. followed by positive molecular characterized (direct sequencing of partial SSU rRNA partial genes and PCR-RFLP at the SSU rRNA). In screened samples were mainly detected parasites named Cryptosporidium spp., found in 4,1% of faecal samples in 2006 and in 2007 in 32,8% faecal samples, out of which 14,4% was found in pre-weaned piglets samples and 26,4% in post-weaned piglets. Based on genotyping provided on positive samples out of the year 2007, using method of sequensing analysis SSU rRNA, was the occurence of C. suis, Cryptosporidium pig genotype II aC. Muris described. High prevalence of Isosporou suis 13,9 % (64/459) was also detected with its appearance, in particular, in pre-weaned piglets 21,4 % (61/285). Further on, some of other identified group was Eimerie spp. 5,7 % (26/459) infecting, in the main, post-weaned piglets 10,9% (19/174) and Giardia intestinalis 2,4 % (10/459). Most of the samples mentioned occured in conditioned faeces and there is no seasonal relationship to the parasital occurance.
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Gut parasites of goats on a chosen farm
VANĚČKOVÁ, Michala
The master thesis is aimed to evaluate intestinal parasites occurrence in a goat breeding. A herd of goats chosen for this survey was kept by a private enterprise focused on milk production. The practical research was conducted during two years, 2009 and 2010. Samples of feaces were collected during that period and analyzed for parasites occurrence. The analyses proved presence of the following parasites: Trichuris ovis, parasites from the order Strongylida, Fasciola hepatica, Dicrocellium lanceatum and coccidia. Total prevalence was significant; 85% of the samples were found to be positive. Species from the order Strongylida were identified as the most often 41,46 %, which meant 199 cases from the total of 509. During the examined period, the highest prevalence of parasites was detected in summer 2009, the second highest then in spring 2010.
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THE OCCURRENCE OF COCCIDIOSIS IN THE GUTS OF SUCKING PIGS BEFORE AND AFTER WEANING.
ČIVIŠOVÁ, Jana
In the whole, 487 samples have been collected from two farms in the Kladno region during the time periods as following: Autumn 2004, Winter 2005, Spring 2005,Autumn 2005, Winter 2006 and Spring 2006. For the investigation of the parasite presence in the dung the flotation-concentration method (Sheather sugar solution) and specific aniline {--} carbolic - methylviolet colouring according to Miláček and Vítovec (1985) with followed microscopy investigation at 200 - 400× magnification have been used. The infections caused by Cryptosporidium spp., Eimeria spp., Isospora suis, Giardia intestinalis, Trichuris suis, Ascaris suum and Strongyloides ransomi have been found in the samples. For the most part, the infection caused by Cryptosporidium spp. (19.1 %), Eimeria spp. and Isospora suis (5.2 %) have occurred in the breeds. Prevalence of the Giardia intestinalis flagellates have reached to 2.9 % and 5.4 % for nematodes Majority of the positive samples had solid consistency of the dung. The smallest occurrence of the parasites have been diagnosed in the winter 2005. On the contrary the greatest occurrence have been diagnosed in autumn 2005.
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