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Píce jako primární zdroj selenu v potravním řetězci
Šišková, Daniela
Selenium (Se) is an essential mineral nutrient for animal and human growth. Defici-ency in this element is a worldwide nutrition problem. Lolium perenne and Trifolium pratense were enriched by selenium using foliar application. It is a way to enrich the feed of animals and human nutrition through animal products. Amounts of 0, 2, 4 and 20mg of Se m-2 were applied both as sodium selenate and as sodium selenite in water. Results showed that selenium concentration in forage was significantly enhanced dose dependently. For Lolium perenne at the highest concentration used, reaching concent-rations of selenium in selenate 3,1371mikrogramm/mg and selenite 5,5503mikrogramm/mg. For Trifolium pratense at the highest concentration used, reaching concentrations of selenium in sele-nate 11,6837mikrogramm/mg and selenite 4,939mikrogramm/mg. Foliar application of selenium was proven to be a good way to increase the selenium content of forage, but the assimilation process of selenium was simpler with selenate for Trifoilum pratense and with selenite for Lolium perenne.
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Identification of filamentous virus which infected red clover (\kur{Trifolium pratense})
BEČKOVÁ, Martina
Samples of red clover plants characteristic with dwarf growth were investigated with transmission electron microscopy. Most frequent virus particles observed there were filamentous ones of 300 to 800 nm long. Nucleic acid was isolated, transcribed and amplified using PCR with potexvirus- and potyvirus-specific primers 353G1, 353G2 (Petrzik, K., unpublished data), P9502, P0502 (Revers et al., 1999) and Poty2/P4 (Gibbs and Mackenzie, 1997). Successful amplification with potexvirus-specific primers, sequencing and comparison with GenBank sequences revealed White clover mosaic virus (WClMV) there. Coat protein gene of this virus was obtained with amplification and compared with complete sequence of WClMV from GenBank. O-strain of WClMV was identified.
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