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Growing Milk thistle (Silybum marianum L. Gaertn.) The content of biologically active substances and their use
HOFÍREK, Pavel
Milk thistle [(Silybum marianum) L. Gaertn] is among the longest known medical plants, described already in the 4th century before Christ. In the first part of my thesis, I studied the technology of cultivation this medical plant. Milk thistle contains biologically active substances. The main active substances are silybin, silydianin, isosilybin, silychristin, known as silymarin complex, and taxifolin. These substances have hepatoprotective effects on liver tissue. Characteristics, some methods of determination of active substances and pharmacological effect I deal with in the second part of my thesis. The content of active substances are influenced by growing technologies and applications elicitors. This issue and current use of plants in human medicine, dietary supplements and feed to attend in the end.
Medicinal plants, their fertilization and elicitors treatment with the aim of maximum production some active substance.
ŠRÁMEK, Jan
Echinacea purpurea belongs to adaptogenic plants containig pharmacologically active compounds. The most important parts of the plants for medical use are roots, but also aboveground parts are processed. The plants were grown in triennial precise small-parcel experiment using acetylsalicylic acid as an in vivo elicitor to increase the content of bioactive substances. The contents of cichoric acid, caffeic acid, chlorogenic acid and caftaric acid were assayed in roots, tops and flowers. Repeated application elicitors on leaves were conducted with three different concentrations in fortnightly intervals. Ethanol extracts from the plant samples were analysed by high performance liquid chromatography. The effect of the elicitor on the content of bioactive substances was statistically significant. The optimal concentration of the elicitor considering the content of bioactive substances in roots is the middle one. The effect of elicitor is year-dependent.

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