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Preventivní a léčebné koupele u plůdku candáta obecného (Sander lucioperca)
PITHARDT, Tomáš
The aim of this thesis was to test and compare the effect of four selected and in present commonly used medical treatments on other species of fish in recirculating aquaculture system (RAS). This presented work was divided into two separate experiments dealing with curative baths for fry of pikeperch.The fry was raised in ponds, feeded by natural food, with the intention of adaptating for RAS after a bath. Baths were targeted against parasitic and bacterial diseases by using Bellasav, SAVO Original, formaldehyde and Chloramine T. The curative baths took 30 minutes after which the fish were transferred to clean water where the mortality within 24 hours and the efficacy of the preparation within 24 hours was monitored. In the first experiment were used fish with an average weight of 0.23+-0.05 g and a total lenght of 31.7+-2.5 mm at the age of 35 days. The tested doses were 0.5; 1; 1.5; 2; 5; 10 and 50 ml.m-3 for Bellasav, 1; 5; 10; 20 and 50 ml.m-3 for SAVO Original, 0.5; 1; 1.5; 2.5; 5; 10; 15; 30; 45 and 50 ml.100 l-1 for formaldehyde and 10; 20; 30; 40; 50; 100; 150 and 200 mg.l-1 for Chloramine T. The best efficacy was achieved at the concentration dose 1.5 ml.m-3 for Bellasav. The concentration dose 10 ml.m-3 of SAVO Original only reduced parasitic infection by one degree. An effective dose of formaldehyde was 1 ml.100 l-1 which reduced the parasitic infection by two degrees. The most effective preparation was Chloramine T at the concentration dose of 30 mg.l-1. In the second experiment were used fish with an average weight of 1.6+-0.4 g and a total lenght of 60.4+-3.56 mm at the age of 70 days. The tested doses were 10; 20 and 50 ml.m-3 for Bellasav, 10; 20 and 50 ml.m-3 for SAVO Original, 2.5; 5 and 10 ml.100 l-1 for formaldehyde and 40; 150 and 200 mg.l-1 for Chloramine T. The best efficacy was achieved at the concentration dose 20 ml.m-3 for Bellasav and 10 ml.m-3 for SAVO Original. The most effective dose for formaldehyde was 2.5 ml.100 l-1 which reduced the bacterial infection in fish skin by two degrees. The most effective preparation was Chloramine T again at the concentration dose 40 mg.l-1.
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Inovativní metody v chovu a reprodukci candáta obecného (Sander lucioperca)
BLECHA, Miroslav
The whole Ph.D. thesis includes in total 10 chapters on 146 pages. Chapter 2 9 are specific parts of thesis where following scientific and practical aspects of pikeperch reproduction biology and aquaculture are described. Quality and quantity of pikeperch spermatozoa after varying cold water treatments are presented in the chapter 2 and can help to optimize broodstock management of males with the aim to obtain high quality spermatozoa during a seasonal and an out of season spawning as well. Benefits of hormone treatment of both sexes in semi-artificial reproduction in pikeperch are described in the chapter 3 where the importance of hormonal treatment of both sexes in tank spawning is evaluated for effective production pikeperch larvae. The use of an alcalase treatment for the elimination of pikeperch egg stickiness is being shown in the chapter 4 of this thesis. Post-ovulatory oocyte ageing and its effect on eggs viability rates and occurrence of larval malformations and ploidy anomalies are listed in chapter 5. These results describe the effects of the egg over ripening fertilization process in pikeperch. The first report of heat shock triploidisation in pikeperch is described in chapter 6 with production of 100% pikeperch triploid population. The effect of water surface treatment on survival, swim bladder inflation and growth of larvae is given in the chapter 7 with the aim to optimize the intensive culture of pikeperch larvae. Last two chapters (8 and 9) are describing the adaptation of intensively cultured juveniles to pond culture and the adaptation of pond-cultured juveniles to RAS as a new and effective methods for ongrowing production of pikeperch. In total, four published scientific papers, one handbook, one accepted scientific paper for publication, and two prepared scientific manuscripts are included and discussed in this Ph.D. thesis.
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Isolation of early stages of germ cells in pikeperch (\kur{Sander lucioperca})
GÜNGÖR, Ege
A practical technique for enzymatic dissociation and isolation of pikeperch (Sander lucioperca) (Percidae, Teleostei) early stage germ cells (eGC), including spermatogonia and spermatocytes, is reported in this study. Their potential to differentiate into functional gametes, and transmit genetic information to the next generations makes them suitable for cryopreservation and surrogate reproduction studies by germline chimera. Two different age groups (14 month old and 18 month old) of pikeperch were used to adjust the correct stage of eGC isolation. Finally the 18 month old samples were selected due to their high average gonadal volume (0.513 g). 10 ml PBS + 0.3% trypsin (304 mOsm/kg, pH 8) were used for enzymatic dissociation of testicular cells and they were sorted by centrifugation in Percoll density gradient. eGCs were identified on the basis of their ploidy level by CYSTAIN DNA 1 steps kit (PARTEC) and morphological characteristics trough by light microscopy. Cell counting was performed on histological sections and Percoll gradient layers whit the method of random square counting. The method of isolation enriched the number of eGC from 41.3% to 84.7%, obtained from the 33% of Percoll concentration.
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Adaptation of Intensively Bred Juvenile Fish of Pikeperch (\kur{Sander lucioperca}) to a Pond Condition
CHOTĚBORSKÝ, Michal
The aim of this study was to evaluate the adaptability of juvenile fish of pikeperch (Sander lucioperca) artificially bred in the recirculation system, which were planted to experimental ponds. Within this study of adaptability, the survival rate of planted fish and their growth in determined period was observed. Later, after several months of breeding the pikeperch in experimental ponds, the returnable adaptability was evaluated, when the fish from ponds were returned to the recirculation system, where they were adapted back to the controlled breeding conditions and artificial feed. Again, the survival rate and growth of adapted fish was evaluated.
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Optimization of harvest of juveniles of pikeperch (\kur{Sander lucioperca}) taken out of ponds and following ability of the fish to adapt to system RAS
HAMPL, Jan
Within my bachelor thesis I have been trying to resolve the possibilities of optimization of harvest of juveniles of pikeperch (Sander lucioperca) taken out of ponds, and continuously I have been tracking the ability of the fish to adapt to system RAS that is applied at the experimental fish culture facilities at FROV JU. Working at the fish harvest it has shown me to be important to synchronize the work of every one of the assistants during the individual activities. The fish have been harvested in a very short time and carefully taken to already prepared transport boxes. We checked the most important parameters for the quality of water, its temperature and oxygen saturation. The mortality of the fish during the harvest and transport did not get over 2 %. Just after the transport to experimental fish culture facilities at FROV JU, to the system RAS at Vodnany, the harvested fish has been placed to 9 tanks for the weaning period. Each out of three ponds always received three tanks of harvested and adapted fish. The experiment at RAS has been divided into three parts. First part has been about the weaning period of the fish to the RAS system and the artificial food. The results were very satisfying. I have been following these parameters: SGR, FCR, increase of biomass, survival and cannibalism at the tanks. The level of survival from the pond Rohlíček has been around 83,6 %, the pond Bejkovna 69,7 % and Horažďovice 4 the level of survival was 71,8 %. After finalizing the weaning period we did biometry for 37 of selected representatives. Second part has been the 1st post-weaning period. Here I focused at the parameters such as SGR, FCR, increase of biomass, survival and cannibalism at the tanks. The level of survival from the pond Rohlíček has been around 84,17 %, the pond Bejkovna 83,83 % and Horažďovice 4 the level of survival was 82,83 %. After finalizing the 1st post-weaning period we did biometry for 37 of selected representatives. Third part has been the 2nd post-weaning period. Here I focused at the same parameters as above such as SGR, FCR, increase of biomass, survival and cannibalism at the tanks. The level of survival from the pond Rohlíček has been around 87,5 %, the pond Bejkovna 88,17 % and Horažďovice 4 the level of survival was 87,17 %. After finalizing the 2nd post-weaning period we did biometry for 37 of selected representatives, the experiment was evaluated and pikeperch juveniles were sold to the French company Asialor.
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