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The use of CAM assay for characterization and study of cancer cell invasive properties
Vágnerová, Lenka ; Dvořák, Michal (advisor) ; Geryk, Josef (referee)
The chorioallantoic membrane (CAM) of chicken embryos belongs to the in vivo model systems frequently used for the study of angiogenesis and cell invasiveness. Using CAM assay we have tested selected chicken sarcoma cell lines characterized by different angiogenic properties and different ability to form metastasis. In addition to CAM assay, several other methods have been used to characterize the phenotype of these cell lines. We have selected a few proteins which could significantly influence the angiogenic and metastatic properties of investigated cell lines. We have established cell lines stably overexpressing these genes and compared their phenotypes with parental cell lines. We have shown that genes encoding ISL1, ARNT2, PROM1, HOXA11 proteins participate, in our experimental model, in activation of programes controlling angiogenesis and cell invasion.
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Model of dynamic contrast CT data for verification of registration algorithms
Kupková, Karolína ; Harabiš, Vratislav (referee) ; Walek, Petr (advisor)
This work is focused on the description of the dynamic contrast-enhanced CT examination and its contribution in the pneumooncology. It includes a program for creating a two-dimensional model of the scan from the thorax and for the perfuse examination simulation using the time-density curve. Real CT data are simulated more authentic using rigid geometric transformations and noise. The model will be used for the validation of registration algorithms that is used to suppress the spatial deformation generated by patient motions during the long time examination.
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CT laser mammography
BAČOVSKÁ, Michaela
According to the data from the Ministry of Health, malignant breast tumours rank in first place in terms of the most frequent malignant tumour disorders in women in the Czech Republic. Every year over 5500 new cases of breast carcinoma are diagnosed, with approximately 2000 women dying each year as a consequence of this disorder. In September 2002 blanket screening for breast cancer was officially launched in the Czech Republic. Its main task resides in identifying breast carcinoma in its early stages, and also in prolonging the survival of patiens due to more effective treatment of the initial stages of the disorder. After the introduction of screening there was logically also an increase in the recorded incidence, and at the same time there should be a reduction of mortality. The endeavour to scan breasts commenced with the development of X-ray examination methods and with the general development of new technologies in the last century diagnostic methods also came to the forefront. Progress was achieved primarily in the area of X-ray examination methods, whilst at the same time there was a parallel development of entirely new methods. Today computer tomography, magnetic resonance or emission computer tomography are entirely available. In terms of scanning breast glands the greatest progress was recorded by mammography, an in close linkage by ultrasound. In addition to these basic and proven methods, the latest development in mammodiagnostics enables us to use also new examination methods, which are used as supplementary methods. One of these is CT laser mammography (CTLM), which is used in several countries worldwide. In the Czech Republic the radiodiagnostice clinic of the Královské Vinohrady Teaching Hospital in Prague and the Masaryk Memorial Cancer Institute in Brno have access to a CTLM machine. As the name indicates, this concerns breast scanning using laser rays. CTLM displays the physiological vessel structure in the breast, as well as any angiogenesis if present.
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