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National Repository of Grey Literature 12 records found  previous11 - 12  jump to record: Search took 0.00 seconds. 
The role of tyrosine phosphorylation in hnRNA splicing
 Koudelková, Lenka ; Brábek, Jan (advisor) ; Kozáková, Eva (referee)
Coding sequences of eukaryotic genes are interrupted by long segments of noncoding intronic DNA, which must be spliced after a transcription into a heterogenous nuclear RNA. Due to an increasing pressure on complexity of proteome eukaryotic organisms evolved alternative splicing. It is enabled through weak consensus sequences of splice sites flanked with accessory regulatory RNA elements, that associate with splicing factors, to create protein products according to current requirements implicated by outer and inner conditions. The net of cooperatively or antagonistic acting factors determines whether splice sites are recognized or not. This molecular system is regulated by enzymatic modifications depending on activity of corresponding signaling pathways. Beside many other enzymes a family of protein tyrosine kinases is involved in the process. Via catalytic activity of their kinase domains, they add phosphate to tyrosines of proteins that participate in RNA metabolism. Phosphorylation affects their affinity for RNA and other interacting partners, localization, enzymatic activity or other properties. The changes result in establishing of new setting of regulatory net and usage of distinct splice sites. Products then may with a different efficiency inhibit or trigger various cell processes or...
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Study of alternatively spliced variants of estrogen receptor alpha in breast cancer cell lines
 Lhota, Filip ; Kleibl, Zdeněk (advisor) ; Souček, Pavel (referee)
Filip Lhota: Study of alternatively spliced variants of estrogen receptor alpha in breast cancer cell lines Abstract: Estrogen receptor α (ER-α) is a transcription factor responsible for mediation of the activities of its natural ligand 17-β-estradiol (E2), the hormone that together with progesterone belongs to the key regulators of mammary epithelial as well as breast cancer cells proliferation. Except to the major gene product consisting of all eight coding exons of ER-α, numerous qualitatively and quantitatively different spliced variants originated from primary transcript by activity of alternative splicing is expressed. Despite that some of these spliced variants have been functionally characterized, their precise role on final ER-α cellular activity remains to be elucidated. The functional characterization of individual alternative forms of ER-α and description of its participation on the overall ER-α activity is important for our understanding of their biogenesis and is also critical for the delineation of molecular bases for ER-α regulation during anti cancer chemotherapy. This work aimed to study the influence of alternatively spliced ER-α variants on the growth characteristics of clones constructed from stable mammary tissue cell lines in regulation to cultivation conditions and cellular...
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National Repository of Grey Literature : 12 records found   previous11 - 12  jump to record:
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