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Evaluation of effectiveness of entomopathogenic fungi Beauveria bassiana using a standard laboratory bioassay
PAULIČ, Radim
In laboratory bioassays, the efficacy of the entomopathogenic fungus Beauveria bassiana against the yellow mealworm (Tenebrio molitor) was tested under various temperature conditions. Six different strains of fungus B. bassiana was investigated. The evaluation was based on vitality bioassays including germination and growth index assessment and the bioassay of virulence based on target organism T. molitor was also assessed growth and yield of conidia different strains of fungus B. bassiana on natural substrates and artificial nutrient substrates.
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Possibilities of AFLP technique in study of \kur{Beauveria bassiana} genetic structure
BERANOVÁ, Kristina
Beauveria bassiana is widely used in biological control against the economically significant pests. Detection of morphological and genetic polymorphism among single species and strains in natural environment is important to study of the distribution and the effects in the ecosystem. The aim of this study was to determine the links of "local strains" with ecological aspects of biological control in protected zone of National park and assess the polymorphism of the population monitored in NP Šumava and exotic strains using molecular markers. There were analyzed 39 strains collected in the NP Šumava and the Krkonoše National Park in the Czech Republic and 11 strains from different countries and areas. The polymorphism of these strains was evaluated by different methods based on DNA analysis. In this study, the genetic variability is evaluated by AFLP markers. The results reveal that population from NP Šumava is very closed and the "local strains" can be very well characterized thanks these methods and compared with other strains from different parts of the Czech Republic and other countries. Thanks to these screening analyses, the strain Bba I101was determined for the preparation which will be used for bark beetle (Ips typographus) control in the National Park Šumava in the Czech Republic. This study was supported by grants GACR 521/08/H042, MSM 60076658-06, MZP SP/2d1/41/08.
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Utilization of PCR-RFLP approach for evaluation of ITS polymorphism in selected \kur{Beauveria bassiana} strains/isolates
KOCKOVÁ, Lucie
The aim of this study was the evaluation of the suitability PCR-RFLP technique and ITS region analysis for determination the genetic variability of the entomopathogenic fungi - Beauveria bassiana, Beauveria brongniartii and Isaria fumosorosea. By the means of ITS-RFLP technique, it was possible to identify the uniformity of the B. bassiana isolates from NP Šumava, the higher level of polymorphism of B.bassiana isolates from different geographical areas was detected and it was possible to distinguish different entomopathogenic fungi used in this study.
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Optimalizacion submerged cultivation of select types enthomopathogenic fungi
SUCHANOVÁ, Michala
This graduation theses was intent on study influence conditions submerged cultivation select types enthomopatogenic fungi in liquid nutritive medium with emphasis on optimalization key elements of the process that manner performance uniform biomass mythosporotic fungi {--} blastospores. Experimental part of work was conceived with regard on next sphere problems: 1.Nutritive soil compositionon effect on production blastospores 2.Comparing possibility different kinds and strains enthomopathogenic fungi produce blastospores in submerged cultivation. 3.Conditions submerged cultivation effect on production and yield blastospores 4.Verify possibility production of uniform biomass blastospores in range usable for large-screen application
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Synergism of entomopathogenic nematodes and entomopathogenic fungi
ŠILLEROVÁ, Tereza
The potential synergism between chosen species of entomopathogenic nematodes (Steinernema arenarium, Steinernema feltiae) and entomopathogenic fungi (Beauveria bassiana, Isaria fumosorosea, Lecanicillium lecanii) is investigated in this study. It is theoretically possible to expect increasing of their efficiency at the collective introduction into environment. Creating of uniform laboratory method which will be possible to use at the research of this interaction system is a part of this study.
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