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Generation of transgenic mouse model to study biological role of KLK5 in epidermis
Kašpárek, Petr ; Sedláček, Radislav (advisor) ; Fafílek, Bohumil (referee)
A number of studies provide strong evidence that KLK5 is one of the most important serine proteases in the epidermis and is involved in processes such as desquamation, processing of antimicrobial peptides or induction of inflammatory reaction. The role of KLK5 has been deduced from in vitro experiments and thus its functions should be verified in vivo. This work aimed to develop a specific tissue targeting strategy to study the role of murine kallikreins in the epidermal compartment in vivo and to generate a transgenic model overexpressing mKlk5 in the mouse epidermis. Using the modified promoter of human involucrin, transgenic mice expressing the fluorescent marker, tdTomato, were generated in the first step. This transgenic reporter mouse showed specific targeting pattern of the reporter in the upper epidermal layers and, thus, the modified involucrin promoter could be employed for targeting further gene of interest in the differentiated epidermal compartment. In the second step transgenic mouse lines expressing murine kallikrein 5 were successfully generated. Among them two lines exhibited approximately 6-9 fold overexpression at the mRNA and protein levels, however it appeared that the immature protease was not activated under normal healthy conditions. Therefore two models of epidermal...
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Problematics of the History education at High schools
Kulhavá, Zdeňka ; Parkan, František (advisor) ; Mikeska, Tomáš (referee)
KEYWORDS: pupil, student's book, didactical analyses, students, student's book function analysis, modern history, tuition, didactical materials The thesis theme is 'The problematics of modern history education at secondary/high schools. The thesis objective is a complex survey of the situation related to the modern history and current history education at grammar schools and specialized secondary/high schools in the Czech Republic. This thesis provides a thorough response to the following questions: Do the teachers at Czech secondary/high schools teach modern history until 1989 or not? Do they reflect the historical period after 1989 and where do they finish? What student's books are used by teachers? What student's books are used by students? What are the conditions for the student's book composition set by the Czech education policy. With an intent to reveal the responses to these questions, the diploma thesis analyses legislative framework of the Czech Republic education policy (Act No. 561/2004 Coll. about pre-school, elementary, secondary/high, college and other education, hereinafter referred to as 'School Act'), basic curricular documents (Framework Educational Program for Elementary Education, Framework Education Program for Grammar Schools, school education programs of particular grammar...
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REACTION OF SOME OXIDOREDUCTASES WITH SELECTED DONORS OF NITRIC OXIDE
Marounková, Běla ; Ryšlavá, Helena (referee)
One of the key functions of nitric oxide (NO) in human is to dilate blood vessels. Glycerol trinitrate (GTN) and other well-known NO donors together with those bearing a >C=N-OH group were tested for possible conversion to NO (or nitrites, respectively) by diaphorase (DP) and lipoamide dehydrogenase (LAD). Both, DP and LAD, were unable to convert Nω- hydroxy-L-arginine (L-NOHA). Although superoxide anion (SOD) participates to a varying extent in the conversion of donors (formaldoxime (FAL), acetaldoxime (AO), nitroprusside (NP), S-nitrosoglutathione (SNOG), S-nitroso-N-acetylpenicillamine (SNAP) and hydroxylamine (HA)), its reactive product, hydrogen peroxide, had no effect on the conversion of these donors. The ratio of formed nitrite/nitrate is important for comparison of the reactions catalyzed by DP and LAD with NO donors.
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Private Labels
Kolmačková, Zuzana ; Cimler, Petr (advisor) ; Hommerová, Dita (referee)
This Bachelor Thesis titled Private labels deals with distribution strategy based on the introduction of private labels especially in retail chains. At the beginning it is focused on the general concept of private label offered by retailers, where is mentioned basic characteristics, history and structuring of distribution brands. Subsequently this thesis informs readers about the introduction of new special distribution brands, which focus primarily on the new consumption habits of customers. The practical part describes the specific relationship between a supply company and two retail companies, with an emphasis on comparing the logistics of the production and promotion of these products, and compares the conditions and standards for the operation of this business relationship.
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Quality of Service in Cellular Networks
Spiššák, Filip ; Mašek, Jan (referee) ; Krkoš, Radko (advisor)
This thesis deals with the quality of service (QoS) mechanism in Evolved Packet System (EPS). The basic architecture and characteristics of the EPS are introduced. The concept of QoS is defined. Furthermore, QoS related entities, their functions and corresponding procedures are described. The methodology for measuring and evaluating of end-to-end QoS as seen from a user’s point of view is designed. This methodology is used to measure QoS of experimental cellular network at UTKO FEEC BUT. Finally, configuration change for optimizing the QoS support of experimental cellular network is designed.
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Optimalization construction tube lifter of spinner machine
Jeník, Jan ; Pavlík, Jan (referee) ; Knoflíček, Radek (advisor)
The first part of this thesis describes the current technologies of mechanical spin-ning. This is a ring, rotor and air-jet spinning. The product of spinning machines is yarn, the basic material for production textile. The next section describes in detail the current state of the node manipulator lifter tube on spinning machine TORNADO J20, by concern RIETER. It describes the structure, kinematics, duty cycle and requirements for the manipulator. The following optimization options which are described more options for solutions of individual functions of the manipulator, on the end is selected by multi-criteria analysis. The best solution that is elaborate in the next section. The author has created 3-D model optimized lifter tube. Positioning mechanism maintains the current kinematics, it is very similar. But gripper was designed as a passive, thus reduce the load positioning mechanism. Optimization was performed primarily to reduce overall production costs and to reduce weight manipulator. The conclusion is a comparison of current and new solutions. There are also eco-nomic recovery to.
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Exocyst subunit AtSEC15b: its role in plant cell morphogenesis and characterization of its Rab interacting partner
Toupalová, Hana ; Žárský, Viktor (advisor) ; Baluška, František (referee) ; Hašek, Jiří (referee)
Organization of endomembrane compartments in all eukaryotic cells is dependent on continuous transport of membrane vesicles. Major part of the core regulators of intracellular membrane transport is represented by small GTPases from the Rab family. Rab GTPases cycle between the GTP-bound "active" and GDP-bound "inactive" forms. In their active form, they are able to interact with specific effectors and perform their functions. Exocyst is an octameric complex involved in regulation of secretion. It functions as an effector of Rab GTPases in yeast and mammals and tethers secretory vesicles to the plasma membrane prior to the actual membrane fusion. Using publicly available expression data, we have identified candidates from Rab GTPase family for the interaction with exocyst subunit AtSEC15b in plants and demonstrated that AtSEC15b specifically interacts with AtRABA4a GTPase. We also showed that, like in yeast and mammals, Arabidopsis Sec15b binds Rab GTPase also probably in GTP-dependent manner, implying that this interaction is well conserved throughout the eukaryotic kingdoms. We also successfully demonstrated the complementation of yeast thermo-sensitive mutant strain, sec15-1. Based on this observation we concluded that AtSEC15b is able to substitute the function of yeast SEC15 and restore the phenotype....
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Regulation of Cathepsin D Activity and Activation
Máša, Martin ; Mareš, Michael (advisor) ; Jonáková, Věra (referee) ; Holada, Karel (referee)
PhD Thesis ABSTRACT REGULATION OF CATHEPSIN D ACTIVITY AND ACTIVATION Martin Máša supervisor: Michael Mareš Department of Biochemistry Faculty of Science Charles University Institute of Organic Chemistry and Biochemistry Academy of Sciences of the Czech Republic PRAGUE 2009 Introduction Cathepsin D (CD) is an aspartic peptidase located in the lysosomes of all mammalian cells, its main role is catabolic degradation of proteins. More over CD is known to participate in a range of physiological processes such as apoptosis and tissue homeostasis, as well as in the regulation of angiogenesis and the production of peptidic antigens. The role of CD in pathophysiology is associated with several diseases such as Alzheimer's disease and cancer. Alzheimer's disease is a neurodegenerative disorder, which is generally considered to be the most common form of dementia. Progression of this disease is accompanied with the deposition of amyloid plaques (AP) in the brain, which leads to neurodegeneration. The AP is a fragment released from amyloid precursor protein (APP) cleaved by secretases1 . High levels of CD were found in cerebrospinal fluid of the Alzheimer's patients2 . It was demonstrated that CD is able to cleave APP and produce the pathogenic AP. A genetic polymorphism in the CD gene was reported, which changes...
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The Influence of eco-toxicological factors on spermatogenesis, morphology and function of mammalian sperm
Vašinová, Jitka ; Pěknicová, Jana (referee) ; Hortová, Kateřina (advisor)
An increasing infertility due to pathological changes on sperm has become a serious issue. A rising concentration of fluorides in the environment could be a potentially negative factor affecting the male fertility. Eco-toxicological effect of fluorides can be enhanced in the present of aluminium ions by forming fluoro-metallic complexes, analogues of phosphate groups that interfere with the activity of G-proteins and P-type ATPases, which are part of several signalling pathways during sperm maturation. The aim of this thesis was monitoring the effect of fluorides on mouse sperm capacitation in vitro, analysis of the effect of fluorides on male reproductive organ and on the level of mouse spermatogenesis. Fluorides were administered to mice through table water at the concentrations of 0, 1, 10, 100 ppm and at the same time we monitored their synergic action with aluminium at 10 ppm dose. The level of tyrosine phosphorylation in the sperm head was the main marker for successful capacitation in vitro. The results show that an oversized load of ions, to which mice were exposed, affected negatively spermatogenesis and ability of sperm to capacitate in vitro. Besides that, a multigenerational study in mice demonstrated that even low concentrations of aluminium fluorides can have a negative effect on...
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Order quantity depending on probability distribution
Naglová, Martina ; Fábry, Jan (advisor) ; Kuncová, Martina (referee)
The thesis examines calculation of the order quantity in case of the stochastic demand. The order quantity is calculated from the cost function and the profit function. In case of the stochastic demand, the exact demand is unknown, but the probability distribution is known. The aim of the thesis is the transformation of the formulas for different probability distributions of the demand. It is necessary to calculate the order quantity so that cost function is minimal. The cost fiction is considered to be bipolar structure where ordering costs are increasing with higher ordering quantity, storage costs are decreasing with higher ordering quantity. In the profit fiction it is examined if it is possible to make higher profits from contracts and how contracts influence the order quantity.
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