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Mobility of photosynthetic proteins
KRAFL, Jaroslav
Mobility of pigment-protein complexes (phycobilizomes and photosystem II playing a key role in photosynthesis) was studied by FRAP method (Fluorescence Recovery After Photobleaching). FRAP represents a fluorescence based microscopy method enabling measurement of protein mobility in living systems. The protein complexes are bleached by a laser pulse. And mobility of unbleached proteins is measured as a fluorescence recovery in the bleached area. Currently we have only limited knowledge about the mobility of photosynthetic proteins. This work was aimed at optimization of the photosynthetic protein mobility measurement by FRAP. I have performed several methodological experiments which led to the successful assessment of phycobilisome and chlorophyll-containing proteins diffusion coefficients in selected red algae (Porfyridium cruentum, Cyanidium caldarium) and cyanobacteria (Synechocystis PCC6803, Acaryochloris marina). The methodology developed and validated in my thesis was then applied in further research projects.
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Cyanobacteria in the splash zone of the Veruda and Ugljan islands, Croatia
VONDRÁŠKOVÁ, Alžběta
Cyanobacterial communities of the splash zone of two Croatian islands, Veruda and Ugljan, were surveyed on 16 localities both in horizontal and vertical directions. In 336 samples 42 cyanobacterial species, 1 green alga, and 1 red alga were found. On both islands Hyella spp. and Gloeocapsopsis crepidinum dominated. Solentia spp., Kyrtuthrix dalmatica, and Mastigocoleus testarum were also found frequently. According to statistical analyses 3 horizontal parts of the splash zone were shown to host different cyanobacterial communities. In the horizontal direction there were no statistical differences among sampling points; however, for uncovering the whole cyanobacterial diversity more samples from various distances are necessary. The species diversity and distribution were not dependent on the orientation of the locality toward cardinal points.
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Cyanobacteria in the splash zone of the Veruda and Ugljan islands, Croatia
VONDRÁŠKOVÁ, Alžběta
Cyanobacterial communities of the splash zone of two Croatian islands, Veruda and Ugljan, were surveyed on 16 localities both in horizontal and vertical directions. In 336 samples 42 cyanobacterial species, 1 green alga, and 1 red alga were found. On both islands Hyella spp. and Gloeocapsopsis crepidinum dominated. Solentia spp., Kyrtuthrix dalmatica, and Mastigocoleus testarum were also found frequently. According to statistical analyses 3 horizontal parts of the splash zone were shown to host different cyanobacterial communities. In the horizontal direction there were no statistical differences among sampling points; however, for uncovering the whole cyanobacterial diversity more samples from various distances are necessary. The species diversity and distribution were not dependent on the orientation of the locality toward cardinal points.
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Eutrophic stress of the Orlik reservoir
ZELENKOVÁ, Kamila
Detection of potential sources of load eutrophic surface waters. Orlik dam is out of your water and energy purposes also used for recreational purposes. In recent years, recorded deterioration of water quality. It happens that it appears "Flower" water surface and increase algae. The content of this work was to monitor the changing state of water quality in reservoirs Orlik and its surroundings during the season. Parameters were monitored: conductivity, pH, oxygen, nitrates, and phosphates and during the season of 2013.
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Methods of reduction of excessive biomass of phytoplankton
CHADTOVÁ, Gabriela
The aim of this study was to prepare review of methods to combat over development of phytoplankton. The work also includes a critical evaluation of usability, efficacy and risks of these methods. The methods to combat over-development of phytoplankton can be divided into direct and indirect. While the direct methods acts directly against phytoplankton, indirect methods are based mostly on reducing the amount of nutrients available for phytoplankton growth. Both of these methods can be further divided to biological, physico-mechanical and chemical. The direct methods of physico-mechanical include ultrasound, flushing and dilution, direct chemical methods include the use of chemical algaecides, coagulants and flocculants. Direct biological methods are especially divided by the principle of parasites(viruses,bacteria and fungi), competitors (algae), allelopathy (extracts from higher plants),predation (protozoa and herbivorous fish). The indirect physico-mechanical methods include extraction and overlapping sediment. Chemical methods are meant by the binding of nutrients to insoluble compound in the sediment and the biological methods are meant use of communities of bacteria for mineralization of sediments. The next part of the work is devoted to the characteristics of algae and cyanobacteria, as well as factors affecting the formation and development of algal blooms. In this part of the work I focus mainly on cyanobacteria, their toxins and the effect they have on the individual components of aquatic ecosystems.
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